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A simple in situ synthesis of iron oxide magnetic nanoparticles embedded in thermosensitive polymer for DNA capture

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Abstract

In this study, we report a simple one-pot synthesis of iron oxide nanoparticles (IONPs) modified with thermoresponsive polymers potentially applicable for nucleic acid capture. Ferrous (Fe2+) and ferric (Fe3+) ions were coprecipitated to a dispersion of previously prepared poly(N-isopropylacrylamide-co-2-aminoethyl methacrylate) P(NIPAAm-co-AEM) for in situ synthesis of magnetite (Fe3O4) and concurrent surface modification of Fe3O4 with the polymer to obtain magnetic nanocomposites. Fourier-transform infrared (FTIR) spectroscopy analysis reveals the surface modification of Fe3O4 with P(NIPAAm-co-AEM) and P(NIPAAm) as functional and control polymers, respectively. Fe3O4@P(NIPAAm-co-AEM) and Fe3O4@P(NIPAAm) nanocomposites’ surfaces contain 7.5 and 2.3 wt% of immobilized polymers, respectively. Vibrating sample magnetometry (VSM) result indicates a high saturation of magnetization value, 75 emu/g, for Fe3O4@P(NIPAAm-co-AEM) nanocomposites. The hydrodynamic diameter of Fe3O4@P(NIPAAm-co-AEM) in water changes depending on pH and temperature. A study for deoxyribonucleic acid (DNA) capture ability of Fe3O4@P(NIPAAm-co-AEM) nanocomposites shows a maximum 18.5 mg/g of DNA can be adsorbed on Fe3O4@P(NIPAAm-co-AEM).

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Acknowledgments

The authors acknowledge the Ministry of Science and Technology, Dhaka, Bangladesh (Project No. PHY's 455) for financial support. We also gratefully acknowledge Mr. Daisuke Matsubara, Yamagata University, Japan for SEM and TEM analyses, and the Central Science Laboratory, Rajshahi University for providing instrumental support. Sadia Hossain is thankful to the Ministry of Science and Technology (MOSICT) Bangladesh for National Science and Technology (NST) fellowship during her M.Sc. study.

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Authors and Affiliations

  1. Research Laboratory of Polymer Colloids & Nanomaterials, Department of Chemistry, Faculty of Science, Rajshahi University, Rajshahi, 6205, Bangladesh

    Sadia Hossain, Mahbubor Rahman, Yeasmin Nahar, Abdur Rahman & Motahar Hossain

  2. Advance Materials Research Laboratory, Department of Materials Science and Engineering, Clemson University, Clemson, SC, 29625-0971, USA

    Mahbubor Rahman

  3. Department of Chemistry, Begum Rokeya University, Rangpur, Bangladesh

    Mostafa Kaiyum Sharafat

  4. Department of Chemistry and Chemical Engineering, Graduate School of Science and Engineering, Yamagata University, Yonezawa, Japan

    Bungo Ochiai

  5. Laboratory of Automatic Control, Chemical and Pharmaceutical Engineering (LAGEP)-Lyon, University of Lyon 1, Villeurbanne Cedex, 69622, France

    Abdelhamid Elaissari & Hasan Ahmad

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  1. Sadia Hossain
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Correspondence to Mahbubor Rahman.

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Hossain, S., Rahman, M., Nahar, Y. et al. A simple in situ synthesis of iron oxide magnetic nanoparticles embedded in thermosensitive polymer for DNA capture. Journal of Materials Research 35, 2441–2450 (2020). https://doi.org/10.1557/jmr.2020.192

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