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Functional glucosamine-iron oxide nanocarriers

  • Biomedical Materials, Regenerative Medicine and Drug Delivery
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Abstract

Herein, we report a synthetic route capable of producing superparamagnetic, stable and biocompatible glucosamine (GLU) nanocarriers, composed by colloidal iron oxide nanoparticles (ION, ~6 nm) surface-functionalized with GLU dispersed in physiological media (pH 7.2). The route consists first of the preparation of ION by aqueous alkaline co-precipitation of 1:2 Fe(II)/Fe(III) followed by surface treatment with citric acid, activation of acidic groups via carbodiimide intermediary and further amidation using GLU as the amine reactant. Results from cell viability tests performed with human dental pulp tissue cells suggest that ION–GLU nanocolloids are biocompatible and non-toxic for two different concentrations and several hours of incubation. Moreover, optical microscopy shows that ION–GLU adsorbs at the cells walls and also transposes them, reaching cytoplasm and nucleus as well. All findings point out the promising use of ION–GLU as biocompatible nanocarriers for GLU delivery such as in articulation diseases.

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Acknowledgments

The financial supports from the Brazilian agencies MCT-CNPq (Process 424152/2016-9), FINEP, FAP-DF (Process: 193.001.358/2016), FINATEC and CAPES are gratefully acknowledged. We are grateful to Dr. M. J. Araújo Sales, Instituto de Quimica - Universidade de Brasília, for her support with TGA measurements. Also, Dr. O. R. Pires Júnior, Instituto de Ciências Biológicas - Universidade de Brasília, is acknowledged for his support with sample lyophilization.

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Authors and Affiliations

  1. Instituto de Física, Universidade de Brasilia, 70910-900, Brasilia, DF, Brazil

    Luis M. R. Rivera, Julhyana G. Machado, Mohan Chandra Mathpal & Maria A. G. Soler

  2. Instituto de Ciências Biológicas, Universidade de Brasilia, 70910-900, Brasilia, DF, Brazil

    Luis M. R. Rivera, Julhyana G. Machado, Natalia L. Chaves, Sônia N. Báo & Ricardo B. Azevedo

  3. Soft Matter Nanotechnology Laboratory, CIC biomaGUNE, 20009, Guipuzkoa, Spain

    Danijela Gregurec & Sergio E. Moya

  4. Instituto de Química, Universidade de Brasilia, 70910-900, Brasilia, DF, Brazil

    Leonardo G. Paterno

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  1. Luis M. R. Rivera
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Rivera, L.M.R., Machado, J.G., Chandra Mathpal, M. et al. Functional glucosamine-iron oxide nanocarriers. Journal of Materials Research 35, 1726–1737 (2020). https://doi.org/10.1557/jmr.2020.121

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