Abstract
3D printing has been shown to be a robust and inexpensive manufacturing tool for a range of applications within biomedical science. Here we report the design and fabrication of a 3D printer-enabled microfluidic device used to generate cell-laden hydrogel microspheres of tunable sizes. An inverse mold was printed using a 3D printer, and replica molding was used to fabricate a PDMS microfluidic device. Intersecting channel geometry was used to generate perfluorodecalin oil-coated gelatin methacrylate (GelMA) microspheres of varying sizes (35–250 µm diameters). Process parameters such as viscosity profile and UV cross-linking times were determined for a range of GelMA concentrations (7–15% w/v). Empirical relationships between flow rates of GelMA and oil phases, microspheres size, and associated swelling properties were determined. For cell experiments, GelMA was mixed with human osteosarcoma Saos-2 cells, to generate cell-laden GelMA microspheres with high long-term viability. This simple, inexpensive method does not require the use of traditional cleanroom facilities and when combined with the appropriate flow setup is robust enough to yield tunable cell-laden hydrogel microspheres for potential tissue engineering applications.
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ACKNOWLEDGMENT
This work was partially supported by the Nappi Family Research Award and CMMI 1547095 (National Science Foundation).
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Suvarnapathaki, S., Ramos, R., Sawyer, S.W. et al. Generation of cell-laden hydrogel microspheres using 3D printing-enabled microfluidics. Journal of Materials Research 33, 2012–2018 (2018). https://doi.org/10.1557/jmr.2018.77
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DOI: https://doi.org/10.1557/jmr.2018.77