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In vivo bone regeneration analysis of trilayer coated 316L stainless steel implant in rabbit model

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Abstract

To increase the corrosion prevention of stainless steel implant and fast recovery due to new bone-cell formation at the orthopedic implant site, in the present investigation, a trilayered (with bioceramic interlayer sandwiched between innermost passivated surface and outermost polymer coating) 316L stainless steel (SS) implant was designed and investigated. It was inferred that this new designed implant invokes faster and more bone-cell formation than uncoated commercially available 316L SS implants. Faster bone-cell formation at the coated implant site reduces the initial threat of implant corrosion. The electrochemical corrosion study proved that this model of coated implants is able to prevent corrosion up to 90% better than uncoated commercially available 316L SS. Subsequently, preclinical studies in the rabbit bone defect model (which included histology, radiology, fluorochrome labeling, push-out test, and scanning electron microscopy taken after 45 and 90 days) proved higher rate of new bone tissue formation and better push-out strength between tissue in contact and the coated implant. The toxicological study of vital organs like liver, kidney, and heart also exhibited no abnormality. The outcome of the experimentations indicates suitability of this trilayered 316L SS implant for bone repair and healing.

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ACKNOWLEDGMENTS

The authors would like to thank Technical Education Quality Improvement Program Phase II (TEQIP-II) for financial support. The authors would also like to thank the Director, CSIR-Central Glass and Ceramic Research Institute, Kolkata, India, and Vice Chancellor, West Bengal University of Animal and Fishery Sciences, Kolkata, India, for their generous and kind support to this work. Dr. Howa Begam (School of Bioscience and Engineering, Jadavpur University, Kolkata, India) and all the personnel involved for characterization of materials are sincerely acknowledged.

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Authors and Affiliations

  1. Department of Metallurgical and Materials Engineering, Jadavpur University, Kolkata, 700032, India

    Priyanka Majee, Shampa Dhar & P. K. Mitra

  2. Department of Veterinary Surgery and Radiology, West Bengal University of Animal and Fishery Sciences, Kolkata, 700037, India

    V. Lalzawmliana & Samit Kumar Nandi

  3. School of Bioscience and Engineering, Jadavpur University, Kolkata, 700032, India

    Piyali Basak

  4. Bioceramics and Coating Division, CSIR-Central Glass and Ceramic Research Institute, Kolkata, 700032, India

    Biswanath Kundu

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  1. Priyanka Majee
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Correspondence to Samit Kumar Nandi or Biswanath Kundu.

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Majee, P., Dhar, S., Mitra, P.K. et al. In vivo bone regeneration analysis of trilayer coated 316L stainless steel implant in rabbit model. Journal of Materials Research 33, 2106–2117 (2018). https://doi.org/10.1557/jmr.2018.119

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  • DOI: https://doi.org/10.1557/jmr.2018.119

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