Abstract
A highly biocompatible peptide, triplet repeats of asparagine-serine-serine (3NSS) regulates mineral deposition for the reconstruction of erosive enamel. Healthy human enamel was demineralized to create lesions, then exposed to the 3NSS peptide solution, and finally immersed in artificial saliva. The degrees of nanohardness recovery were 5.02% and 16.27% for the control group and enamel treated with the 3NSS peptide, respectively. Peptides assembling at enamel interred attracted greater quantities of ions from the solution to form nanocrystalline hydroxyapatite minerals during the reconstruction of vacant gap. This resulted in a decrease in the surface roughness, and the acidic eroded pores were filled completely. Additionally, the newly deposited hydroxyapatites remineralized with the aid of the 3NSS peptide exhibited a smaller average crystalline size, which effectively inhibited plastic deformations. Treatment with the 3NSS peptide provided great improvements in nanohardness and elastic modulus.
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T. Aoba: Solubility properties of human tooth mineral and pathogenesis of dental caries. Oral Dis. 10, 249 (2004).
J. van Houte: Role of microorganisms in caries etiology. J. Dent. Res. 73, 672 (1994).
S.V. Dorozhkin and M. Epple: Biological and medical significance of calcium phosphates. Angew. Chem. Int. Ed. 41, 3130 (2002).
E.C. Reynolds: Remineralization of enamel subsurface lesions by casein phosphopeptide-stabilized calcium phosphate solutions. J. Dent. Res. 76, 1587 (1997).
Y. Iijima, F. Cai, P. Shen, G. Walker, C. Reynolds, and E.C. Reynolds: Acid resistance of enamel subsurface lesions remineralized by a sugar-free chewing gum containing casein phosphopeptide-amorphous calcium phosphate. Caries Res. 38, 551 (2004).
K.J. Cross, N.L. Huq, J.E. Palamara, J.W. Perich, and E.C. Reynolds: Physicochemical characterization of casein phosphopeptide-amorphous calcium phosphate nanocomplexes. J. Biol. Chem. 280, 15362 (2005).
M.V. Morgan, G.G. Adams, D.L. Bailey, C.E. Tsao, S.L. Fischman, and E.C. Reynolds: The anticariogenic effect of sugar-free gum containing CPP-ACP nanocomplexes on approximal caries determined using digital bitewing radiography. Caries Res. 42, 171 (2008).
M. Hannig and C. Hannig: Nanomaterials in preventive dentistry. Nat. Nanotechnol. 5, 565 (2010).
Y.R. Cai and R.K. Tang: Calcium phosphate nanoparticles in biomineralization and biomaterials. J. Mater. Chem. 18, 3775 (2008).
M.S. Tung and F.C. Eichmiller: Amorphous calcium phosphates for tooth mineralization. Comp. Cont. Educ. Dent. 25, 9 (2004).
J. Kirkham, A. Firth, D. Vernals, N. Boden, C. Robinson, R.C. Shore, S.J. Brookes, and A. Aggeli: Self-assembling peptide scaffolds promote enamel remineralization. J. Dent. Res. 86, 426 (2007).
S. Segman-Magidovich, H. Grisaru, T. Gitli, Y. Levi-Kalisman, and H. Rapaport: Matrices of acidic beta-sheet peptides as templates for calcium phosphate mineralization. Adv. Mater. 20, 2156 (2008).
A. George, L. Bannon, B. Sabsay, J.W. Dillon, J. Malone, A. Veis, N.A. Jenkins, J.G. Debra, and G.C. Neal: The carboxyl-terminal domain of phosphophoryn contains unique extended triplet amino acid repeat sequences forming ordered carboxyl-phosphate interaction ridges that may be essential in the biomineralization process. J. Biol. Chem. 271, 32869 (1996).
C.C. Hsu, H.Y. Chung, J.M. Yang, W. Shi, and B. Wu: Influence of 8DSS peptide on nano-mechanical behavior of human enamel. J. Dent. Res. 90, 88 (2011).
H.Y. Chung, C.C. Li, and C.C. Hsu: Characterization of the effects of 3DSS peptide on remineralized enamel in artificial saliva. J. Mech. Behav. Biomed. Mater. 6, 74 (2012).
E.L. Norcy, S.Y. Kwak, F.B. Wiedemann-Bidlack, E. Beniash, Y. Yamakoshi, J.P. Simmer, and H.C. Margolis: Leucine-rich amelogenin peptides regulate mineralization in vitro. J. Dent. Res. 90, 1091 (2011).
R.I. Holland: Corrosion testing by potentiodynamic polarization in various electrolytes. Dent. Mater. 8, 241 (1992).
W.C. Oliver and G.M. Pharr: An improved technique for determining hardness and elastic-modulus using load and displacement sensing indentation experiments. J. Mater. Res. 7, 1564 (1992).
J. Zhou and L.L. Hsiung: Biomolecular origin of the rate-dependent deformation of prismatic enamel. Appl. Phys. Lett. 89, 51904 (2006).
T. Moriguchi, K. Yano, S. Nakagawa, and F. Kaji: Elucidation of adsorption mechanism of bone-staining agent alizarin red S on hydroxyapatite by FT-IR microspectroscopy. J. Colloid Interface Sci. 260, 19 (2003).
J. Reyes-Gasga, R. García-García, M.J. Arellano-Jiménez, E. Sanchez-Pastenes, G.E. Tiznado-Orozco, I.M. Gil-Chavarria, and G. Gómez-Gasga: Structural and thermal behaviour of human tooth and three synthetic hydroxyapatites from 20 to 600 °C. J. Phys. D. Appl. Phys. 41, 225407 (2008).
G.K. Toworfe, R.J. Composto, I.M. Shapiro, and P. Ducheyne: Nucleation and growth of calcium phosphate on amine-, carboxyl- and hydroxyl-silane self-assembled monolayers. Biomaterials 27, 631 (2006).
H. Fong, M. Sarikaya, S.N. White, and M.L. Snead: Nano-mechanical properties profiles across dentin-enamel junction of human incisor teeth. Mater. Sci. Eng., C 7, 119 (2000).
J.L. Cuy, A.B. Mann, K.J. Livi, M.F. Teaford, and T.P. Weihs: Nanoindentation mapping of the mechanical properties of human molar tooth enamel. Arch. Oral Biol. 47, 281 (2002).
G. Balooch, G.W. Marshall, S.J. Marshall, O.L Warren, S.A.S. Asif, and M. Balooch: Evaluation of a new modulus mapping technique to investigate microstructural features of human teeth. J. Biomech. 37, 1223 (2004).
S. Roy and B. Basu: Mechanical and tribological characterization of human tooth. Mater. Charact. 59, 747 (2008).
C.C. Hsu, H.Y. Chung, J.M. Yang, W. Shi, and B. Wu: Influences of ionic concentration on nanomechanical behaviors for remineralized enamel. J. Mech. Behav. Biomed. Mater. 4, 1982 (2011).
J.A. Cury, G.S. Simões, A.A. Del Bel Cury, N.C. Gonçalves, and C.P.M. Tabchoury: Effect of a calcium carbonate-based dentifrice on in situ enamel remineralization. Caries Res. 39, 255 (2005).
L.E. Bertassoni, S. Habelitz, S.J. Marshall, and G.W. Marshall: Mechanical recovery of dentin following remineralization in vitro: An indentation study. J. Biomech. 44, 176 (2011).
N. Srinivasan, M. Kavitha, and S.C. Loganathan: Comparison of the remineralization potential of CPP–ACP and CPP–ACP with 900ppm fluoride on eroded human enamel: An in situ study. Arch. Oral Biol. 55, 541 (2010).
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Chung, HY., Li, CC. Asparagine-serine-serine peptide regulates enamel remineralization in vitro. Journal of Materials Research 28, 2890–2896 (2013). https://doi.org/10.1557/jmr.2013.292
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DOI: https://doi.org/10.1557/jmr.2013.292