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A versatile method for generating single DNA molecule patterns: Through the combination of directed capillary assembly and (micro/nano) contact printing

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Abstract

One of the challenges in the development of molecular scale devices is the integration of nano-objects or molecules onto desired locations on a surface. This integration comprises their accurate positioning, their alignment, and the preservation of their functionality. Here, we proved how capillary assembly in combination with soft lithography can be used to perform DNA molecular combing to generate chips of isolated DNA strands for genetic analysis and diagnosis. The assembly of DNA molecules is achieved on a topologically micropatterned polydimethylsiloxane stamp inducing almost simultaneously the trapping and stretching of single molecules. The DNA molecules are then transferred onto aminopropyltriethoxysilane-coated surfaces. In fact, this technique offers the possibility to tightly control the experimental parameters to direct the assembly process. This technique does not induce a selection in size of the objects, therefore it can handle complex solutions of long (tens of kbp) but also shorter (a few thousands of bp) molecules directly in solution to allow the construction of future one-dimensional nanoscale building templates.

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ACKNOWLEDGMENTS

This work was supported by the EC-funded project NaPa (Contract No. NMP4-CT-2003-500120). We thank the “Région Midi-Pyrénées” council, and the “Institut des Technologies Avancées en sciences du Vivant (ITAV)” for their financial support. We also thank Jean-Marie François and his research group, especially Marie-Ange Teste from the “Engineering of Biological Systems and Process Laboratory”.

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Authors and Affiliations

  1. CNRS, LAAS, F-31077 Toulouse, France; and Université de Toulouse, UPS, INSA, INP, ISAE, LAAS, F-31077, Toulouse, France

    Aline Cerf & Xavier Dollat

  2. Physics Department, University of Minnesota, Minneapolis, Minnesota, 55416, France

    Jérôme Chalmeau

  3. Université de Toulouse, INSA, UPS, INP, LISBP, Toulouse, F-31077, France

    Angélique Coutable

  4. CNRS, LAAS, F-31077, Toulouse, France

    Christophe Vieu

  5. CNRS, LAAS, F-31077, Toulouse, France

    Jérôme Chalmeau

  6. Université de Toulouse, UPS, INSA, INP, ISAE, LAAS, F-31077, Toulouse, France

    Jérôme Chalmeau

  7. INRA, UMR792 Ingénierie des Systèmes Biologiques et des Procédés, CNRS, UMR5504, Toulouse, F-31400, France

    Angélique Coutable

  8. Université de Toulouse, UPS, INSA, INP, ISAE, LAAS, F-31077, Toulouse, France

    Christophe Vieu

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  1. Aline Cerf
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  2. Xavier Dollat
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Correspondence to Aline Cerf.

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Cerf, A., Dollat, X., Chalmeau, J. et al. A versatile method for generating single DNA molecule patterns: Through the combination of directed capillary assembly and (micro/nano) contact printing. Journal of Materials Research 26, 336–346 (2011). https://doi.org/10.1557/jmr.2010.12

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  • DOI: https://doi.org/10.1557/jmr.2010.12

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