Abstract
There exists a need for an innovative reconstructive approach for breast reconstruction, tackling current drawbacks and limitations present in the clinic. In this respect, adipose tissue engineering could offer a promising alternative. We have previously shown that methacrylamide-functionalized gelatin scaffolds are suitable to support the adhesion of adipose tissue-derived stem cells as well as their subsequent differentiation into the adipogenic lineage. The current paper aims to compare different techniques to produce such scaffolds including direct versus indirect 3D printing. Extrusion-based (direct) 3D printing was compared to indirect 3D printing exploiting a polylactic acid (PLA) sacrificial mould, thereby focussing on the physico-chemical characteristics of the obtained scaffolds. The results indicate that similar properties can be achieved irrespective of the technique applied. It can therefore be concluded that indirect 3D printing could offer some benefits over direct additive manufacturing (AM) as a more complex design can be created while materials that were previously unsuited for direct printing because of limitations associated with their characteristics (e.g. low viscosity), could potentially be applied as starting materials for indirect 3D printing to generate porous constructs with full control over their design.
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F. Bray, Jacques Ferlay, Isabelle Soerjomataram, R. L. Siegel, L. A. Torre, and A. Jemal, “Global Cancer Statistics 2018: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries,” CA. Cancer J. Clin., 2018.
A. Hassan El-Sabbagh, “Modern trends in lipomodelling,” GMS Interdiscip. Plast. Reconstr. Surg. DGPW, vol. 6, no. April, 1998.
J. Roostaeian, L. Pavone, A. Da Lio, J. Lipa, J. Festekjian, and C. Crisera, “Immediate placement of implants in breast reconstruction: Patient selection and outcomes,” Plast. Reconstr. Surg., vol. 127, no. 4, pp. 1407–1416, 2011.
M. Vaezi, G. Zhong, H. Kalami, and S. Yang, “Extrusion-based 3D printing technologies for 3D scaffold engineering,” Mater. Technol. Appl., pp. 235–254, 2018.
D. A. Young and K. L. Chustman, “Injectable biomaterials for adipose tissue engineering,” Biomed. Mater., vol. 7, no. 2, pp. 1–17, 2012.
E. Ruoslahti, “Rgd and Other Recognition Sequences for Integrins,” Annu. Rev. Cell Dev. Biol, vol. 12, no. 1, pp. 697–715, 1996.
T. Chen, H. D. Embree, L. Q. Wu, and G. F. Payne, “In vitro protein-polysaccharide conjugation: Tyrosinase-catalyzed conjugation of gelatin and chitosan,” Biopolymers, vol. 64, no. 6, pp. 292–302, 2002.
T. Billiet, E. Gevaert, T. De Schryver, M. Cornelissen, and P. Dubruel, “The 3D printing of gelatin methacrylamide cell-laden tissue-engineered constructs with high cell viability,” Biomaterials, vol. 35, no. 1, pp. 49–62, 2014.
L. Tytgat et at, “Additive manufacturing of photo-crosslinked gelatin scaffolds for adipose tissue engineering,” Acta Biomater., 2019.
L. Flynn and K. A. Woodhouse, “Adipose tissue engineering with cells in engineered matrices,” Organogenesis, vol. 4, no. 4, pp. 228–235, 2008.
S. Peltola, F. Melchels, D. Grijpma, and M. Kellomaki, “A review of rapid prototyping techniques for tissue engineering purposes.,” AnnMed, vol. 40, no. 4, pp. 268–80, 2008.
C. Z. Liu, E. Sachlos, D. A. Wahl, Z. W. Han, and J. T. Czernuszka, “On the manufacturability of scaffold mould using a 3D printing technology,” Rapid Prototyp. J., 2007.
M. Rumpler, A. Woesz, J. W. C. Dunlop, J. T. Van Dongen, and P. Fratzl, “The effect of geometry on three-dimensional tissue growth,” J. R. Soc. Interface, 2008.
J. Van Hoorick et at, “(Photo-)crosslinkable gelatin derivatives for biofabrication applications,” Acta Biomater., 2019.
C. De Maria, A. De Acutis, and G. Vozzi, “Indirect rapid prototyping for tissue engineering,” in Essentials of 3D Biofabrication and Translation, 2015.
J. Van Hoorick et at, “Indirect additive manufacturing as an elegant tool for the production of self-supporting low density gelatin scaffolds,” J. Mater. Sci. Mater. Med., 2015.
A. I. Van Den Bulcke, B. Bogdanov, N. De Rooze, E. H. Schacht, M. Cornelissen, and H. Berghmans, “Structural and rheological properties of methacrylamide modified gelatin hydrogels,” Biomacromolecules, vol. 1, no. 1, pp. 31–38, 2000.
D. Yoo, “New paradigms in hierarchical porous scaffold design for tissue engineering,” Mater. Sci. Eng. C, vol. 33, no. 3, pp. 1759–1772, 2013.
S. Ansari et at, “Hydrogel elasticity and microarchitecture regulate dental-derived mesenchymal stem cell-host immune system cross-talk,” Acta Biomater., vol. 60, pp. 181–189, 2017.
H. Shih, T. Greene, M. Korc, C. Lin, W. Lafayette, and B. Simon, “Modular and adaptable tumor niche prepared from visible light-initiated thiol-norbornene photopolymerization,” vol. 17, no. 12, pp. 3872–3882, 2017.
N. C. Negrini, P. Tarsini, M. C. Tanzi, and S. Fare, “Chemically crosslinked gelatin hydrogels as scaffolding materials for adipose tissue engineering,” J. Appl. Polym. Sci., vol. 47104, pp. 1–12, 2019.
P. Y. Huri, B. A. Ozilgen, D. L. Hutton, and W. L. Grayson, “Scaffold pore size modulates in vitro osteogenesis of human adipose-derived stem/stromal cells,” Biomed. Mater., vol. 9, no. 4, 2014.
L. Tytgat et at, “Evaluation of 3D printed gelatin-based scaffolds with varying pore size for MSC-based adipose tissue engineering,” Macromot Biosci., 2020.
S. Hong et at, “3D Printing of Highly Stretchable and Tough Hydrogels into Complex, Cellularized Structures,” Adv. Mater., 2015.
J. Odent, T. J. Wallin, W. Pan, K. Kruemplestaedter, R. F. Shepherd, and E. P. Giannelis, “Highly Elastic, Transparent, and Conductive 3D-Printed Ionic Composite Hydrogels,” Adv. Funct. Mater., 2017.
K. Holzl, S. Lin, L. Tytgat, S. Van Vilerberghe, L. Gu, and A. Ovsianikov, “Bioink properties before, during and after 3D bioprinting,” Biofabrication, vol. 8, pp. 1–19, 2016.
A. Do, B. Khorsand, S. M. Geary, and A. K. Salem, “3D Printing of Scaffolds for Tissue Regeneration Applications,” Adv Heal. Mater, vol. 4, no. 12, pp. 1742–1762, 2015.
J. W. Nichol and A. Khademhosseini, “Modular tissue engineering: Engineering biological tissues from the bottom up,” Soft Matters, vol. 5, no. 7, pp. 1312–1319, 2010.
A. L. Rutz, K. E. Hyland, A. E. Jakus, W. R. Burghardt, and R. N. Shah, “A multimaterial bioink method for 3D printing tunable, cell-compatible hydrogels,” Adv. Mater., vol. 27, no. 9, pp. 1607–1614, 2015.
F. Urciuolo, G. Imparato, A. Totaro, and P. A. Netti, “Building a tissue in vitro from the bottom up: Implications in regenerative medicine.,” Methodist Debakey Cardiovasc. J., vol. 9, no. 4, pp. 213–217, 2013.
R. Tiruvannamalai-Annamalai, D. R. Armant, and H. W. T. Matthew, “A glycosaminoglycan based, modular tissue scaffold system for rapid assembly of perfusable, high cell density, engineered tissues,” PLoS One, vol. 9, no. 1, 2014.
A. Goyanes, M. Kobayashi, R. Martinez-Pacheco, S. Gaisford, and A. W. Basit, “Fused-filament 3D printing of drug products: Microstructure analysis and drug release characteristics of PVA-based caplets,” Int. J. Pharm., 2016.
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Van Damme, L., Briant, E., Blondeel, P. et al. Indirect versus direct 3D printing of hydrogel scaffolds for adipose tissue regeneration Lana Van Damme, Emilie Briant, Phillip Blondeel, Sandra Van Vlierberghe. MRS Advances 5, 855–864 (2020). https://doi.org/10.1557/adv.2020.117
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DOI: https://doi.org/10.1557/adv.2020.117