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Super-Critical-CO2 De-ECM Process

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Abstract

Extracellular Matrix (ECM), a natural biomaterials, have recently garnered attention in tissue engineering for their high degree of cell proliferative capacity, biocompatibility, biodegradability, and tenability in the body. Decellularization process offers a unique approach for fabricating ECM-based natural scaffold for tissue engineering application by removing intracellular contents in a tissue that could cause any adverse host responses. The effects of Supercritical carbon dioxide (Sc-CO2) treatment on the histological and biochemical properties of the decellularized extracellular matrix (de-ECM) were evaluated and compared with de-ECM from conventional decellularization process to see if it offers significantly reduced treatment times, complete decellularization, and well preserved extracellular matrix structure. The study has shown that a novel method of using supercritical fluid extraction system indeed removed all unnecessary residues and only leaving ECM. The potential of Sc-CO2 de-ECM progressed as a promising approach in tissue repair and regeneration.

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Authors and Affiliations

  1. Center for Printable Materials Certification, The University of Texas at El Paso, El Paso, TX, USA

    Diana Cho & Namsoo P. Kim

  2. Materials and Biomedical Engineering Department, The University of Texas at El Paso, El Paso, TX, USA

    Seungwon Chung, Jaeseok Eo & Namsoo P. Kim

Authors
  1. Diana Cho
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  2. Seungwon Chung
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  3. Jaeseok Eo
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  4. Namsoo P. Kim
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Cho, D., Chung, S., Eo, J. et al. Super-Critical-CO2 De-ECM Process. MRS Advances 3, 2391–2397 (2018). https://doi.org/10.1557/adv.2018.494

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