Abstract
Understanding how biomineralization occurs in the extracellular matrix (ECM) of bone cells is crucial to the development of a successfully engineered bone tissue scaffold, and to date there has not been a well-established method for the quantitative examination of bone mineralization in situ. We investigated the mechanical properties of MC3T3-E1 osteoblast-like cells and the crystalline properties of their biomineralized ECM in vitro using shear modulation force microscopy (SMFM), confocal laser scanning microscopy (CLSM), synchrotron X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FTIR). The elastic modulus of the mineralizing cells increased at time points corresponding to mineral production, whereas that of the non-mineralizing cells did not vary significantly over time. CLSM showed a restructuring of the F-actin fiber network of mineralizing cells with time, which indicates remodeling activities in the cytoskeleton and was not seen in the non-mineralizing cells. Both XRD and FTIR showed that the mineralizing subclone produced hydroxyapatite in situ and that the non-mineralizing subclone was in fact weakly biomineralizing.
Similar content being viewed by others
References
http://www.cdc.gov/ncipc/factsheets/falls.htm [Center for Disease Control and Prevention. 2005. National Center for Injury Prevention and Control.]
L. F. Bonewald, S. E. Harris, J. Rosser et al., Calcified Tissue International 72 (5), 537 (2003).
H. A. Declercq, R. M. H. Verbeeck, Lifjm De Ridder et al., Biomaterials 26 (24), 4964 (2005).
S. Ge, Y. Pu, W. Zhang et al., Physical Review Letters 85 (11), 2340 (2000).
R. Marcus, D. Feldman, and J. Kelsey, Osteoporosis. (Academic Press Inc., 1996).
E. Takai, K.D. Costa, A. Shaheen et al., Annals of Biomedical Engineering 33 (7), 963 (2005).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Meng, Y., Ba, X., Kwak, SY. et al. Effect of Substrate Surface Modification on Biomineralization of Osteoblasts. MRS Online Proceedings Library 950, 1009 (2006). https://doi.org/10.1557/PROC-0950-D10-09
Received:
Accepted:
Published:
DOI: https://doi.org/10.1557/PROC-0950-D10-09