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Ultrastructural analyses of nanoscale apatite biomimetically grown on organic template

Journal of Materials Research volume 23, pages 478–485 (2008)Cite this article

Abstract

The ultrastructure of nanoscale apatite biomimetically formed on an organic template from a supersaturated mineralizing solution was studied to examine the morphological and crystalline arrangement of mineral apatites. Needle-shaped apatite crystal plates with a size distribution of ∼100 to ∼1000 nm and the long axis parallel to the c axis ([002]) were randomly distributed in the mineral films. Between these randomly distributed needle-shaped apatite crystals, amorphous phases and apatite crystals (∼20–40 nm) with the normal of the grains quasi-perpendicular to the c axis were observed. These observations suggest that the apatite film is an interwoven structure of amorphous phases and apatite crystals with various orientations. The mechanisms underlying the shape of the crystalline apatite plate and aggregated apatite nodules are discussed from an energy-barrier point of view. The plate or needle-shaped apatite is favored in single-crystalline form, whereas the granular nodules are favored in the polycrystalline apatite aggregate. The similarity in shape in both single-crystalline needle-shaped apatite and polycrystalline granular apatite over a wide range of sizes is explained by the principle of similitude, in which the growth and shape are determined by the forces acting upon the surface area and the volume.

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Acknowledgments

We acknowledge the support from National Institutes of Health Grants R01 DE 013380 and DE 015411 (to D.H. Kohn). S.I. Hong is grateful for support from the Korea Research Foundation (2004-D00318).

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Affiliations

  1. Department of Biologic and Materials Sciences, University of Michigan, Ann Arbor, Michigan, 48109-1078, USA

    S.I. Hong & D.H. Kohn

  2. Department of Nano-materials Engineering, Chungnam National University, Taejon, 305-764, Korea

    S.I. Hong & K.H. Lee

  3. Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan, 48109-2099, USA

    M.E. Outslay & D.H. Kohn

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  1. S.I. Hong
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  2. K.H. Lee
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  3. M.E. Outslay
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  4. D.H. Kohn
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Correspondence to S.I. Hong.

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Hong, S., Lee, K., Outslay, M. et al. Ultrastructural analyses of nanoscale apatite biomimetically grown on organic template. Journal of Materials Research 23, 478–485 (2008). https://doi.org/10.1557/JMR.2008.0051

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  • DOI: https://doi.org/10.1557/JMR.2008.0051