Summary
Piperine, an alkaloid with diverse biological activity commonly occurring in fruits of Piper sp., has high commercial, economical, and medicinal value. In this communication densitometric estimation of piperine from fruits of Piper nigrum L., processed Piper nigrum L. (white pepper), Piper longum L., Piper retrofractum Vahl, Piper cubeba Hunter, and Piper betle L. is reported. Extracts of these fruits and a standard solution of piperine were applied to silica gel F254 HPTLC plates and the plates were developed in a twin-trough chamber with toluene-ethyl acetate-diethyl ether 6:3:1 as mobile phase. The plates were scanned at 337 nm and quantification of piperine was based on a predetermined calibration plot obtained by chromatography of 15 to 75 ng standard. The quantity of piperine was highest in fruits of Piper nigrum L. and least in Piper betle L. The trend was Piper nigrum L. > Piper longum L. > processed Piper nigrum L. > Piper retrofractum Vahl > Piper cubeba Hunter > Piper betle L. The HPTLC method was found to enable simple, convenient, rapid screening and quantification of the active marker piperine in six fruits of Piper sp. commonly used in the Indian traditional system of medicine.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
V.S. Parmar, S.C. Jain, K.S. Bisht, R. Jain, P. Taneja, A. Jha, O.D. Tyagi, A.K. Prasad, J. Wengel, C.E. Olsen, P.M. Boll, Phytochemistry 46 (1997) 591–673.
N. Singh, V.K. Kulshrestha, R.K. Srivastava, R.P. Kohli, J. Res. Indian. Med. 8 (1973b) 21–26.
B.G. Bhat, N. Chandrasekhara, Nahrung 3 (1987) 913–916.
A.M. Mujumdar, J.N. Dhuley, V.K. Deshmukh, P.H. Raman, S.L. Thorat, S.R. Naik, Indian J. Exp. Biol. 28 (1990) 486–87.
A.M. Mujumdar, J.N. Dhuley, V.K. Deshmukh, S.R. Naik, Indian Drugs 36 (1999) 123–126.
S.K. Desai, V.S. Gawali, A.S. Naik, L.L. D’souza, Int. J. Pharm. 4 (2008) 393–397.
A.M. Mujumdar, J.N. Dhuley, V.K. Deshmukh, P.H. Raman, S.R. Naik, Jap. J. Med. Sci. Biol. 43 (1990) 95–100.
J.N. Dhuley, P.H. Raman, A.M. Mujumdar, S.R. Naik, Indian J. Exp. Biol. 31 (1993) 443–445.
M.B. Daware, A.M. Mujumdar, S. Ghaskadbi, Planta Med. 66 (2000) 231–236.
S. Bajad, K.L. Bedi, A.K. Singla, R.K. Johri, Planta Med. 67 (2001) 284–287.
M. Aggarwal, B.L. Kaul, Indian Drugs 29 (1992) 447–449.
V.R. Karekar, A.M. Mujumdar, S.S. Joshi, J.N. Dhuley, S.L. Shinde, S.M. Ghaskadbi, Arzneim.-Forsch./Drug Res. 46 (1996) 972–975.
U.M.V. Sarma, P.V. Srinivas, V. Anuradha, J.M. Rao, J. Planar Chromatogr. 19 (2006) 238–240.
D. Kulkarni, S.P. Apte, F. Mary, R.T. Sane, Indian Drugs 38 (2001) 323–326.
C. Marutoiu, I. Gogoasa, I. Oprean, O.-F. Marutoiu, M.-I. Moise, C. Tigae, M. Rade, J. Planar Chromatogr. 19 (2006) 250–252.
N. Kanaki, M. Dave, H. Padh, M. Rajani, J. Nat. Med. 62 (2008) 281–283.
A.C. Sutar, D.P. Sohoni, M.M. Banavalikar, M.K. Biyani, Indian Drugs 40 (2003) 692–694.
K.K. Rout, O.P. Rout, S.K. Mishra, J. Planar Chromatogr. 20 (2007) 447–450
J.K. Verma, A.V. Joshi, J. Planar Chromatogr. 19 (2006) 398–400.
M.S. Saumy, N.D. Grampurohit, Indian Drugs 39 (2002) 101–105.
N.S. Jeganathan, K. Kannan, Indian J. Pharm. Educ. Res. 42 (2008) 59–64.
V. Jain, S. Saraf, S. Saraf, Asian J. Chem. 19 (2007) 5331–5335.
N.S. Jeganathan, K. Kannan, Asian J. Chem. 20 (2008) 1621–1626.
S. Manimaran, S.S. Raja, S. Gulshan, L. Parul, M.J. Nanjan, K. Cinnaswamy, B. Suresh, Indian Drugs 42 (2005) 802–804.
G.B. Bhat, N. Chandrasekhara, J. Chromatogr. 338 (1985) 259–263.
ICH Guidelines on Analytical Method Validation, in: Proc. Int. Convention on Quality for the Pharmaceutical Industry, Toronto, September 2002.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Rajopadhye, A.A., Upadhye, A.S. & Mujumdar, A.M. HPTLC Method for Analysis of Piperine in Fruits of Piper Species. JPC-J Planar Chromat 24, 57–59 (2011). https://doi.org/10.1556/JPC.24.2011.1.11
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1556/JPC.24.2011.1.11