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Densitometric HPTLC Method for Analysis of Triterpenoids in the Leaves of Jovibarba sobolifera (Sims.) Opiz (Hen and Chickens Houseleek)

JPC – Journal of Planar Chromatography – Modern TLC volume 22, pages 367–369 (2009)Cite this article

Summary

High performance thin-layer chromatography (HPTLC) combined with densitometry has been used for analysis of the triterpenoid content of acetone and ethyl acetate extracts of the leaves of Jovibarba sobolifera (Sims.) Opiz. After optimization of the extraction process, HPTLC separation was successfully standardized, using silica gel plates washed with methanol and dichloromethane, dichloromethane-ethyl acetate 18.5:1.5 as mobile phase, and 8% H2SO4 in ethanol (m/m) as spray reagent. Triterpenoids in the leaves of Jovibarba sobolifera were quantified by measurement of absorbance. Three spots were observed on chromatograms - the sum of α and β-amyrin, oleanolic acid, and an unidentified compound. All of the triterpenoid components were analyzed for the first time in this plant. The triterpenoid content was calculated statistically.

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Authors and Affiliations

  1. Department of Pharmaceutical Botany, Faculty of Pharmacy, Medical University of Lublin, Chodźki 1, 20-093, Lublin, Poland

    Katarzyna Szewczyk

  2. Department of Medicinal Chemistry, Faculty of Pharmacy, Medical University of Lublin, Jaczewskiego 4, 20-090, Lublin, Poland

    Łukasz Komsta

  3. Department of Chemistry, Laboratory of Planar Chromatography, Faculty of Pharmacy, Medical University of Lublin, Staszica 6, 20-081, Lublin, Poland

    Agnieszka Skalska-Kamińska

Authors
  1. Katarzyna Szewczyk
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  2. Łukasz Komsta
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  3. Agnieszka Skalska-Kamińska
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Correspondence to Katarzyna Szewczyk.

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Szewczyk, K., Komsta, Ł. & Skalska-Kamińska, A. Densitometric HPTLC Method for Analysis of Triterpenoids in the Leaves of Jovibarba sobolifera (Sims.) Opiz (Hen and Chickens Houseleek). JPC-J Planar Chromat 22, 367–369 (2009). https://doi.org/10.1556/JPC.22.2009.5.10

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  • DOI: https://doi.org/10.1556/JPC.22.2009.5.10

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