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Densitometric HPTLC Method for Analysis of Triterpenoids in the Leaves of Jovibarba sobolifera (Sims.) Opiz (Hen and Chickens Houseleek)


High performance thin-layer chromatography (HPTLC) combined with densitometry has been used for analysis of the triterpenoid content of acetone and ethyl acetate extracts of the leaves of Jovibarba sobolifera (Sims.) Opiz. After optimization of the extraction process, HPTLC separation was successfully standardized, using silica gel plates washed with methanol and dichloromethane, dichloromethane-ethyl acetate 18.5:1.5 as mobile phase, and 8% H2SO4 in ethanol (m/m) as spray reagent. Triterpenoids in the leaves of Jovibarba sobolifera were quantified by measurement of absorbance. Three spots were observed on chromatograms - the sum of α and β-amyrin, oleanolic acid, and an unidentified compound. All of the triterpenoid components were analyzed for the first time in this plant. The triterpenoid content was calculated statistically.

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Correspondence to Katarzyna Szewczyk.

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Szewczyk, K., Komsta, Ł. & Skalska-Kamińska, A. Densitometric HPTLC Method for Analysis of Triterpenoids in the Leaves of Jovibarba sobolifera (Sims.) Opiz (Hen and Chickens Houseleek). JPC-J Planar Chromat 22, 367–369 (2009).

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