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Densitometric HPTLC Method for Analysis of Triterpenoids in the Leaves of Jovibarba sobolifera (Sims.) Opiz (Hen and Chickens Houseleek)

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Summary

High performance thin-layer chromatography (HPTLC) combined with densitometry has been used for analysis of the triterpenoid content of acetone and ethyl acetate extracts of the leaves of Jovibarba sobolifera (Sims.) Opiz. After optimization of the extraction process, HPTLC separation was successfully standardized, using silica gel plates washed with methanol and dichloromethane, dichloromethane-ethyl acetate 18.5:1.5 as mobile phase, and 8% H2SO4 in ethanol (m/m) as spray reagent. Triterpenoids in the leaves of Jovibarba sobolifera were quantified by measurement of absorbance. Three spots were observed on chromatograms - the sum of α and β-amyrin, oleanolic acid, and an unidentified compound. All of the triterpenoid components were analyzed for the first time in this plant. The triterpenoid content was calculated statistically.

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References

  1. R. Hegnauer, Chemotaxonomie Der Pflanzen, Band 3, Birkhäuser Verlag, Basel und Stuttgart, 1964, 572–584.

    Book  Google Scholar 

  2. Z. Mirek, H. Piękoś-Mirkowa, M. Zając, Flowering plants and Pteridophytes of Poland, A Checklist, W. Szafer Institute of Botany, Polish Academy of Sciences, 2002.

    Google Scholar 

  3. Flora Europaea, Vol. 1, Psilotaceae to Plantaceae, Cambridge University Press, 1993, 422–429.

    Google Scholar 

  4. J.F. Stevens, H. Hart, R.T. Elema, A. Bolck, Phytochemistry 41 (1996) 503–512.

    Article  CAS  Google Scholar 

  5. V. Abram, M. Donko, J. Agric. Food Chem. 47 (1999) 485–489.

    Article  CAS  Google Scholar 

  6. M. Sentjurc, M. Nemec, H.D. Connor, V. Abram, J. Agric. Food Chem. 51 (2003) 2766–2771.

    Article  CAS  Google Scholar 

  7. R. Xu, G.C. Fazio, S.P.T. Matsuda, Phytochemistry 65 (2004) 261–291.

    Article  CAS  Google Scholar 

  8. R Development Core Team, R: A Language and Environment for Statistical Computing, R Foundation for Statistical Computing, Vienna, Austria, ISBN 3-900051-07-0, URL, 2005.

    Google Scholar 

  9. Sz. Nyiredy, Planar Chromatography - A Retrospective View for the Third Millennium, Springer, Budapest, 2001.

    Google Scholar 

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Authors and Affiliations

  1. Department of Pharmaceutical Botany, Faculty of Pharmacy, Medical University of Lublin, Chodźki 1, 20-093, Lublin, Poland

    Katarzyna Szewczyk

  2. Department of Medicinal Chemistry, Faculty of Pharmacy, Medical University of Lublin, Jaczewskiego 4, 20-090, Lublin, Poland

    Łukasz Komsta

  3. Department of Chemistry, Laboratory of Planar Chromatography, Faculty of Pharmacy, Medical University of Lublin, Staszica 6, 20-081, Lublin, Poland

    Agnieszka Skalska-Kamińska

Authors
  1. Katarzyna Szewczyk
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  2. Łukasz Komsta
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  3. Agnieszka Skalska-Kamińska
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Correspondence to Katarzyna Szewczyk.

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Szewczyk, K., Komsta, Ł. & Skalska-Kamińska, A. Densitometric HPTLC Method for Analysis of Triterpenoids in the Leaves of Jovibarba sobolifera (Sims.) Opiz (Hen and Chickens Houseleek). JPC-J Planar Chromat 22, 367–369 (2009). https://doi.org/10.1556/JPC.22.2009.5.10

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  • DOI: https://doi.org/10.1556/JPC.22.2009.5.10

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