Journal of Flow Chemistry

, Volume 4, Issue 2, pp 66–71 | Cite as

Investigating the Kinetics of Antibody Adsorption onto Polyethylene Terephthalat (PET) Modified with Gold Nanoparticles in Flow Microchannel

  • Mohammed Kechadi
  • Mathilde Faure
  • Bruno Sotta
  • Jean Gamby
Full Paper


The real-time monitoring of the adsorption kinetic of antibody onto polyethylene terephthalate (PET) modified with gold nanoparticles (NPs) is performed into a dielectric flow microchannel. The principle is based on the microelectrode-dielectric interface excitation by a modulated voltage excitation through the dielectric layer with high frequency range. The set-up configuration using a homemade current-to-voltage converter has been developed for fast monitoring of biomolecule adsorption without direct electrical contact of microelectrodes with the microchannel flow. The change in the interfacial admittance, related to the output voltage measured through the microchip, during antibody incubation, gives information on the kinetics involved while antibodies are adsorbed at the interface. An example of adsorption on PET modified with gold NPS was taken. Experimental data were fit with the Langmuir equation and a good correlation was obtained with this latter, where the equilibrium constant, K, was found as 1.55 × 108 M−1 with a limit of detection for antibody concentration without depletion equal to 8.25 nM.


PET microchannel admittance gold nanoparticles antibody adsorption 


  1. 1.
    Homola, J. Anal. Bioanal. Chem. 2003, 377(3), 528–539.CrossRefGoogle Scholar
  2. 2.
    Höök, F.; Rodahl, M.; Brzezinski, P.; Kasemo, B. Langmuir 1998, 14, 729–734.CrossRefGoogle Scholar
  3. 3.
    Bizet, K.; Gabrielli, C.; Perrot, H. Appl Biochem. Biotechnol. 2000, 89, 139–150.CrossRefGoogle Scholar
  4. 4.
    Lazerges, M.; Perrot, H.; Niriniony, R.; Antoine, E.; Compere, C. Chem. Commun. 2005, 48, 6020–6022.CrossRefGoogle Scholar
  5. 5.
    Lazerges, M.; Perrot, H.; Antoine, E.; Defontaine, A.; Compere, C. Biosens. Bioelectron. 2006, 21, 1355–1358.CrossRefGoogle Scholar
  6. 6.
    Gamby, J.; Lazerges, M.; Pernelle, C.; Perrot, H.; Girault, H. H.; Tribollet, B. Lab Chip 2007, 7, 1607–1609.CrossRefGoogle Scholar
  7. 7.
    Gamby, J.; Lazerges, M.; Girault, H. H.; Deslouis, C.; Gabrielli, C.; Perrot, H.; Tribollet, B. Anal. Chem. 2008, 80, 8900–8907.CrossRefGoogle Scholar
  8. 8.
    Bandey, H. L.; Martin, S. J.; Cernosek, R. W.; Hillman, A. R. Anal. Chem. 1999, 71, 2205–2214.CrossRefGoogle Scholar
  9. 9.
    Zhou, C.; Friedt, J. M.; Angelova, A.; Choi, K. H.; Laureyn, W.; Frederix, F.; Francis, L. A.; Campitelli, A.; Engelborghs, Y.; Borghs, G. Langmuir, 2004, 20, 5870–5878.CrossRefGoogle Scholar
  10. 10.
    Gamby, J.; Abid, J.-P.; Girault, H. H. J. Am. Chem. Soc. 2005, 127, 13300–13304.CrossRefGoogle Scholar
  11. 11.
    Gamby, J.; Abid, J.-P.; Abid, M.; Ansermet, J.-P.; Girault, H. H. Anal. Chem. 2006, 78, 5289–5295.CrossRefGoogle Scholar
  12. 12.
    Gamby, J.; Rudolf, A.; Abid, M.; Girault, H. H.; Deslouis, C.; Tribollet, B. Lab Chip 2009, 9, 1806–1808.CrossRefGoogle Scholar
  13. 13.
    Faure, M.; Kechadi, M.; Sotta, B.; Gamby, J.; Tribollet, B. Electroanalysis 2013, 25, 1151–1158.CrossRefGoogle Scholar
  14. 14.
    Kechadi, M.; Gamby, J.; Chaal, L.; Girault, H. H.; Saidani, B.; Tribollet, B. Electrochim. Acta. 2013, 105, 7–14.CrossRefGoogle Scholar
  15. 15.
    Kechadi, M.; Gamby, J.; Chaal, L.; Saidani, B.; Tribollet, B. J. Flow Chem. 2013 3, 81–86.CrossRefGoogle Scholar
  16. 16.
    Gamby, J.; Abid, J.-P.; Tribollet, B.; Girault, H. H. Small 2008, 4, 802–809.CrossRefGoogle Scholar
  17. 17.
    Turkevich, J.; Stevenson, P. C.; Hillier J. Discuss. Faraday Soc. 1951, 11, 55–75.CrossRefGoogle Scholar
  18. 18.
    Moreno, E. C.; Kresak, M.; Kane, J. J.; Hay, D. I. Langmuir 1987, 3, 511–519.CrossRefGoogle Scholar
  19. 19.
    Lan, Q. D.; Bassi, A. S.; Zhu, J. X.; Margaritis, A. Chem. Engineer. J. 2001, 81, 179–186.CrossRefGoogle Scholar
  20. 20.
    Skidmore, G. L.; Horstmann, B. J.; Chase, H. A. J. Chromatogr. 1990, 498, 113–128.CrossRefGoogle Scholar
  21. 21.
    Lionello, A.; Josserand, J.; Jesen, H.; Girault, H. H. Lab Chip 2005, 5, 1096–1103.CrossRefGoogle Scholar
  22. 22.
    Delahay, P.; Trachtenberg, I. J. Am. Chem. Soc. 1957, 79, 2355–2362.CrossRefGoogle Scholar
  23. 23.
    Reinmuth, W. H. J. Phys. Chem. 1961, 65, 473.CrossRefGoogle Scholar
  24. 24.
    Lionello, A.; Josserand, J.; Jesen, H.; Girault, H. H. Lab Chip 2005, 5, 254–260.CrossRefGoogle Scholar
  25. 25.
    Zemann, A. J.; Schnell, E.; Volgger, D.; Bonn, G. K. Anal. Chem. 1998, 70, 563–567.CrossRefGoogle Scholar
  26. 26.
    Da Silva, J. A. F.; do Lago, C. L. Anal. Chem. 1998, 70, 4339–4343.CrossRefGoogle Scholar
  27. 27.
    Tuma, P.; Opekar, F.; Jelinek, I. Electroanalysis 2001, 13, 989–992.CrossRefGoogle Scholar
  28. 28.
    Wang, J. Electroanalysis 2005, 17, 1133–1140.CrossRefGoogle Scholar
  29. 29.
    Pumera, M. Talanta 2007, 74, 358–364.CrossRefGoogle Scholar
  30. 30.
    Opekar, F.; Tuma, P.; Stulik K. Sensors 2013, 13, 2786–2801.CrossRefGoogle Scholar
  31. 31.
    Liu, Y.; Lu, H.; Zhong, W.; Song, P.; Kong, J.; Yang, P.; Girault, H. H.; Liu, B. Anal. Chem. 2006, 78, 801–808.CrossRefGoogle Scholar
  32. 32.
    Liu, Y.; Zhong, W.; Meng, S.; Kong, J.; Lu, H.; Yang, P.; Girault, H. H.; Liu, B. Chem. — Eur. J. 2006, 12, 6585–6591.CrossRefGoogle Scholar
  33. 33.
    Liu, Y.; Xue, Y.; Ji, J.; Chen, X.; Kong, J.; Yang, P.; Girault, H. H.; Liu, B. Mol. Cell. Proteomics 2007, 6, 1428–1436.CrossRefGoogle Scholar

Copyright information

© Akadémiai Kiadó 2013

Authors and Affiliations

  • Mohammed Kechadi
    • 1
    • 2
  • Mathilde Faure
    • 1
    • 2
  • Bruno Sotta
    • 3
  • Jean Gamby
    • 1
    • 2
  1. 1.Laboratoire Interfaces et Systèmes Electrochimiques (LISE, case courrier 133)CNRS, UPR 15ParisFrance
  2. 2.Laboratoire Interfaces et Systèmes Electrochimiques (LISE)UPMC Université Paris 06, UPR 15ParisFrance
  3. 3.UPMC, UR 5, Laboratoire de Physiologie cellulaire et Moléculaire des PlantesParisFrance

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