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Journal of Flow Chemistry

, Volume 4, Issue 2, pp 66–71 | Cite as

Investigating the Kinetics of Antibody Adsorption onto Polyethylene Terephthalat (PET) Modified with Gold Nanoparticles in Flow Microchannel

  • Mohammed Kechadi
  • Mathilde Faure
  • Bruno Sotta
  • Jean Gamby
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Abstract

The real-time monitoring of the adsorption kinetic of antibody onto polyethylene terephthalate (PET) modified with gold nanoparticles (NPs) is performed into a dielectric flow microchannel. The principle is based on the microelectrode-dielectric interface excitation by a modulated voltage excitation through the dielectric layer with high frequency range. The set-up configuration using a homemade current-to-voltage converter has been developed for fast monitoring of biomolecule adsorption without direct electrical contact of microelectrodes with the microchannel flow. The change in the interfacial admittance, related to the output voltage measured through the microchip, during antibody incubation, gives information on the kinetics involved while antibodies are adsorbed at the interface. An example of adsorption on PET modified with gold NPS was taken. Experimental data were fit with the Langmuir equation and a good correlation was obtained with this latter, where the equilibrium constant, K, was found as 1.55 × 108 M−1 with a limit of detection for antibody concentration without depletion equal to 8.25 nM.

Keywords

PET microchannel admittance gold nanoparticles antibody adsorption 

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Copyright information

© Akadémiai Kiadó 2013

Authors and Affiliations

  • Mohammed Kechadi
    • 1
    • 2
  • Mathilde Faure
    • 1
    • 2
  • Bruno Sotta
    • 3
  • Jean Gamby
    • 1
    • 2
  1. 1.Laboratoire Interfaces et Systèmes Electrochimiques (LISE, case courrier 133)CNRS, UPR 15ParisFrance
  2. 2.Laboratoire Interfaces et Systèmes Electrochimiques (LISE)UPMC Université Paris 06, UPR 15ParisFrance
  3. 3.UPMC, UR 5, Laboratoire de Physiologie cellulaire et Moléculaire des PlantesParisFrance

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