Abstract
There are two main methods for estimating seed density and species composition of soil seed banks: manual seed extraction and seedling emergence. These methods were used to determine and compare seed density and species composition in the soil of a natural grassland in Patagonia. Additionally, known amounts of seeds of different sizes from Patagonian grassland species were mixed with soil to evaluate the efficiency of the seed extraction method, and determine their recovery percentage. Seed density found in the grassland soil with the extraction method was four times higher than that found with the seedling emergence method. Through the use of these two methods, there was very little overlap found in species composition. Small seeds (< 1 mm) were only found with the seedling emergence method, whereas the seeds of species with specific germination requirements were found with the seed extraction method. Seed recovery of grassland species varied from 2.5% for smaller seeds (Erophila verna) to 100% for larger seeds (Rumex acetosella) with the seed extraction method. This method was more effective in detecting seeds of large-seeded species. Discrepancies in seed detection between both methods may be related to seed dormancy, specific germination requirements, seed size and mass. These two methods are necessary to describe seed density and seed bank composition.
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References
Ball, D.A. and S.D. Miller. 1989. A comparison of techniques for estimation of arable soil seedbanks and their relationship to weed flora. Weed Res. 29:365–373.
Baskin, C.C. and J.M. Baskin. 1998. Seed Ecology, Biogeography and Evolution of Dormancy and Germination. Academic Press, San Diego.
Bernhardt, K.G., M. Koch, M. Kropf, E. Ulbel and J. Webhofer. 2008. Comparison of two methods characterizing the seed bank of amphibious plants in submerged sediments. Aquatic Bot. 88:171–177.
Borza, J.K., P.R. Westerman and M. Liebman. 2007. Comparing estimates of seed viability in three foxtail (Setaria) species using the imbibed seed crush test with and without additional tetra-zolium testing. Weed Technology 21:518–522.
Brown, D. 1992. Estimating the composition of a forest seed bank: a comparison of the seed extraction and seedling emergence methods. Can. J. Bot. 70:1063–1612.
Correa, M.N. 1969–1999. Flora patagónica. Varios Volúmenes, Colección Cíentfica INTA, Buenos Aires, Argentina.
de Villiers, A. J., M.W. Van Rooyen and G.K. Theran. 1994. Comparison of two methods for estimating the size of the viable seed bank of two plant communities in the Strandveld of the west coast, South Africa. South Afr. J. Bot. 60:81–84.
Ferrandis, P., J.M. Herranz and J.J. Martínez-Sánchez. 1999. Fire impact on a maquis soil seed bank in Cabañeros National Park (Central Spain). Israel J. Plant Sci. 47:17–26.
Ghermandi, L. 1992. Caracterización del banco de semillas de una estepa en el noroeste de la Patagonia. Ecol. Austral. 2:39–46.
Ghermandi, L. and S. Gonzalez. 2009. Diversity and functional groups dynamics affected by drought and fire in Patagonian grasslands. Ecoscience 16:408–417.
Gonzalez, S. and L. Ghermandi. 2008. Postfire seed bank dynamics on semiarid grasslands. Plant Ecol. 199:175–185.
Gonzalez, S., J. Franzese and L. Ghermandi. 2010. Role of fire on Patagonian grasslands: changes in aboveground vegetation and soil seed bank. In: M. Haider and T. Müller (eds.), Advances in Environmental Research. Vol. II. Nova Science Publishers, Hauppauge, NY. pp. 243–264.
Gross, K.L. 1984. Effects of seed size and growth form on seedling establishment of six monocarpic perennial plants. J. Ecol. 72:369–387.
Gross, K.L. 1990. A comparison of methods for estimating soil seed banks. J. Ecol. 78:1079–1093.
Gross, K.L. and K.A. Renner. 1989. A new method for estimating seed numbers in the soil. Weed Sci. 37:836–839.
Ishikawa-Goto, M. and S. Tsuyusaki. 2004. Methods of estimating seed banks with reference to long-term seed burial. J. Plant Res. 117:245–248.
Leck, M.A., K.P. Parker and R.L. Simpson. 1989.The Ecology of Soil Seed Banks. Academic Press, San Diego, CA.
Malone, C.R. 1967. A rapid method for enumeration of viable seeds in soil. Weeds 15:381–382.
Manders, P.T. 1990. Soil seed banks and post-fire seed deposition across a forest-fynbos ecotone in the Cape Province. J. Veg. Sci. 1:491–498.
Mesgaran, M.B., H.R. Mashhadi, E. Zand and H.M. Alizadeh. 2007. Comparison of three methodologies for efficient seed extraction in studies of soil weed seedbanks. Weed Res. 47:472–478.
Plue, J., K. Thompson, K. Verheyen and M. Hermy. 2012. Seed banking in ancient forest species: why total sampled area really matters. Seed Sci. Res. 22:123–133.
Price, J.N., B.D. Wright, C.L. Gross and W.R.D.B. Whalley. 2010. Comparison of seedling emergence and seed extraction for estimating the composition of soil seed banks. Methods Ecol. Evol. 2:151–157.
Roberts, H.A. 1981. Seedbanks in soil. Adv. Appl. Biol. 6:1–55.
Thompson, K. and J.P Grime. 1979. Seasonal variation in the seed banks of herbaceous species in ten contrasting habitats. J. Ecol. 67: 893–921.
Tsuyuzaki, S. 1994. Rapid seed extraction from soils by a flotation method.. Weed Res. 34: 433–436.
Warr, S.J., K. Thompson and M. Kent. 1993. Seed banks as a neglected area of biogeographic research: a review of literature and sampling techniques. Progr. Phys. Geogr. 17:329–347.
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Gonzalez, S.L., Ghermandi, L. Comparison of methods to estimate soil seed banks: the role of seed size and mass. COMMUNITY ECOLOGY 13, 238–242 (2012). https://doi.org/10.1556/ComEc.13.2012.2.14
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DOI: https://doi.org/10.1556/ComEc.13.2012.2.14