The objectives of this research were to examine the inheritance of leaf rust resistance genes in the Iranian wheat cultivar ‘Marvdasht’, which is highly resistant to leaf and stripe rusts, and to identify Lr genes present in this cultivar using molecular markers. The genetic basis of resistance to the leaf rust pathogen (Puccinia triticina) in ‘Marvdasht’ was studied in F2:3 populations derived from crosses of Bolani (susceptible cultivar) × Marvdasht. Isolates 84-1 and 85-28 of P. triticina, which are the predominant isolates in Iran, were used to examine the segregation of resistance originating from ‘Marvdasht’. The results indicated that resistance in ‘Marvdasht’ to Puccinia triticina isolate 84-1 was governed by two dominant seedling resistance genes Lr1 and Lr17a. Allelism studies using an F2 population derived from a cross between ‘Falat’ (Seri 82) and Marvdasht indicated that resistance in Marvdasht was not due to the resistance gene Lr26 present in ‘Falat’. With the application of a previously developed molecular marker for Lr1, the STS marker RGA-567-5, the presence of Lr1 was verified in Marvdasht. Based on bulk segregant analysis, Lr17a was mapped to the distal end of chromosome 2AS and was closely linked to microsatellite marker Xbarc212 at a distance of 3.7 cM. In conclusion, the presence of Lr1 and Lr17a was confirmed in the cultivar Marvdasht.
Al-Maaroof, E.M., Singh, R.P., Huerta-Espino, J., Rattu, A. 2005. Resistance of some Iraqi bread wheat cultivars to Puccinia triticina. Phytopathol. Meditarr. 44:247–255.
Anonymous, 2005. Annual Report for Cereal Diseases in Iran. Seed and Plant Improvement Institute, Karaj, Iran.
Barcellos, A.L., Roelfs, A.P., Moraes-Fernandes, M.I.B. 1999. Inheritance of adult plant leaf rust resistance in the Brazilian wheat cultivar Toropi. Plant Dis. 84:90–93.
Bremenkamp-Barrett, B., Faris, J.D., Fellers, J. P. 2008. Molecular mapping of the leaf rust resistance gene Lr17a in wheat. Crop Sci. 48:1124–1128.
Cloutier, S., McCallum, B.D., Loutre, C., Banks, T.W., Wicker, T., Feuillet, C., Keller, B., Jordan, M.C. 2007. Leaf rust resistance gene Lr1, isolated from bread wheat (Triticum aestivum L.) is a member of the large psr567 gene family. Plant Mol. Biol. 65:93–106.
Dyck, P.L., Kerber, E.R. 1985. Resistance of the race-specific type. In: Roelfs, A.P., Bushnell, W.R. (eds), The Cereal Rusts. Academic Press, Orlando, USA, pp. 469–500.
Flor, H.H. 1971. Current status of the gene-for-gene concept. Annu. Rev. Phytopathol. 8:275–296.
Gupta, S., Saini, R.G., Gupta, A.K. 1995. Genetic analysis of resistance to leaf rust pathotypes in durum wheats PBW34 and DWL5023. Plant Breed. 114:176–178.
Khanna, R., Bansal, U.K., Saini, R.G. 2005. Genetic of durable resistance to leaf rust and stripe rust of an Indian wheat cultivar HD2009. J. Appl. Genet. 46:259–263.
Kolmer, J. A. 1996. Genetics of resistance to wheat leaf rust. Ann. Rev. Phytopathol. 34:435–455.
Kolmer, J. A., Liu, J.Q. 2002. Inheritance of leaf rust resistance in the wheat cultivars AC Majestic, AC Splendor and AC Karma. Canadian J. of Plant Pathol. 24:324–333.
Liu, J.Q., Kolmer, J.A. 1997. Inheritance of leaf rust resistance in wheat cultivars Grandin and CDC Teal. Plant Dis. 81:505–508.
Mago, R., Miah, H., Lawrence, G.J., Wellings, C.R., Spielmeyer, W., Barriana, H.S., McIntosh, R.A., Pryor, A.J., Ellis, J.G. 2005. High resolution mapping and mutation analysis separate the rust resistance genes Sr31, Lr26 and Yr9 on the short arm of rye chromosome. Theor. Appl. Genet. 112:41–50.
McIntosh, R.A., Wellings, C.R., Park, R.F. 1995. Wheat Rusts: An Atlas of Resistance Genes. CSIRO Publications, Victoria, Australia.
McIntosh, R.A., Devos, K.M., Dubcovsky, J., Rogers, W.J., Morris, C.F., Appels, R., Somers, D.J., Anderson, O.A. 2008. Catalogue of Gene Symbols for Wheat, 2008 Supplement. Available online at http://wheat.pw.usda.gov/ggpages/wgc/2008upd.pdf
Mesterházy, A., Bartos, P., Goyeau, H., Niks, R.E., Csösz, M., Andersen, O., Casulli, F., Ittu, M., Jones, E., Manisterski, J., Manninger, K., Pasquini, M., Rubiales, D., Schachermayr, G., Strzembicka, A., Szunics, L., Todorova, M., Unger, O., Vanco, B., Vida, G., Walther, U. 2000. European virulence survey for leaf rust in wheat. Agronomie 20:793–804.
Oelke, L.M., Kolmer, J.A. 2005. Genetics of leaf rust resistance in spring wheat cultivars Alsen and Norm. Phytopathol. 95:773–778.
Rafiei, F., Arzani, A., Afshari, F., Torabi, M. 2007. Characterization of leaf rust resistance genes in seedlings of wheat cultivars. Genetic and Breeding 36:19–27.
Singh, D., Park, R.F., Barriana, H.S., McIntosh, R.A. 2001. Cytogenetic studies in wheat XIX. Chromosome location and linkage studies of a gene for leaf rust resistance in the Australian cultivar “Harrier”. Plant Breed. 120:7–12.
Singh, R.P., William, H.M., Huerta-Espino, J., Rosenwarene, G. 2004. Wheat rust in Asia: Meeting the challenges with old and new technologies. In: Proceedings of the 4th International Crop Science Congress, Brisbane, Australia, Published in CDROM, Available on internet: www.cropscience.org.au
Singh, R.P., Rajaram, S. 1991. Resistance to Puccinia recondita f. sp. tritici in 50 Mexican bread wheat cultivars. Crop Sci. 31:1472–1479.
Somers, D.J., Issac, P., Edwards, K. 2004. A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor. Appl. Genet. 109:1105–1114.
Zhang, J.X., Singh, R.P., Kolmer, J.A., Huerta-Espino, J., Jin, Y., Anderson, J.A. 2008. Inheritance of leaf rust resistance in the CIMMYT wheat Weebill 1. Crop Sci. 48:1037–1047.
Communicated by J. Kolmer
About this article
Cite this article
Rafiei Boroujeni, F., Arzani, A., Afshari, F. et al. Identification and inheritance of leaf rust resistance genes in the wheat cultivar ‘Marvdasht’. CEREAL RESEARCH COMMUNICATIONS 39, 67–76 (2011). https://doi.org/10.1556/CRC.39.2011.1.7
- bulk segregation analysis
- infection type
- near isogenic line