Abstract
A new unstable organ-specific Adh1 mutant was isolated from autotetraploid S 25 Wf9 maize line after germinating kernels under water for two days. Adh1-Fm4x-1 expresses normal and/or reduced levels of ADH1 in anaerobic scutellum. No activity or approximately 33–75% of wild type level of ADH1 in pollen grains was observed. The organ-specific phenotype of the Adh1-Fm4x-1 could be maintained by selfing for ten subsequent generations. After crossing with the wild-type allele the Adh1 mutation transmitted to the next generation both by female and male gametophytes. The appearance of one- and two-banded patterns in anaerobic scutellum and pollen grains of heterozygous F 1 plants showed the lack of F·F homodimers.
DNA sequence analyses of the wild type allele, the Adh1-Fm4x-1 mutant allele and four F 1 revertants revealed that the Adh1-Fm4x-1 mutation contains a Dissociation (Ds1) transposable element in the 5′ untranslated leader of the maize Adh1 gene, which regulates organ-specific expression at a quantitative level.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Chen, C.-H., Freeling, M., Mercklebach, A. 1986. Enzymatic and morphological consequences of Ds excisions from maize Adh1. Maydica (McClintock issue) 31:93–108.
Chen, C.-H., Olshi, K.K., Kloeckener-Gruissem, B., Freeling, M. 1987. Organ-specific expression of maize Adh1 is altered after a Mu transposon insertion. Genetics 116:469–477.
Davis, R.J. 1964. Disc electrophoresis 2. Method of application to human serum proteins. A.N.Y. Acid. Sci. 121:404–427.
Dawe, R.K., Lachmansingh, A.R., Freeling, M. 1993. Transposon-mediated mutations in the untranslated leader of maize Adh1 that increase and decrease pollen-specific gene expression. Plant Cell 5:311–319.
Freeling, M. 1976. Intragenic recombination in maize: Pollen analysis methods and the effect of parental Adh1 isoalleles. Genetics 83:701–717.
Freeling, M., Cheng, S.-K.D., Alleman, M. 1982. Mutant alleles that are altered in quantitative, organ-specific behavior. Dev. Genet. 3:179–196.
Hajósné, Novák M., H. Nagy, A. 1995. Az alkohol dehidrogenáz-1 (Adh1) gén kifejeződése tetraploid kukorica (Zea mays L.) pollenjében. [Expression of alcohol-dehydrogenase-1 (Adh1) gene in the pollen grains of autotetraploid maize (Zea mays L.)]. Növénytermelés 44:229–235.
Kloeckner-Guissem, B., Vogel, J.M., Freeling, M. 1992. The TATA box promoter region of maize Adh1 affects its organ-specific expression. The EMBO Journal 11:157–166.
Schwartz, D. 1969. An example of gene fixation resulting from selective advantage in suboptimal conditions. Am. Nat. 103:479–481.
Schwartz, D., Osterman, J. 1976. A pollen selection system for alcohol-dehydrogenase negative mutants in plants. Genetics 83:63–65.
Sutton, W.D., Gerlach, W.L., Schwartz, D., Peacock, W.J. 1984. Molecular nature of Ds controlling element mutations at the Adh1 locus of maize. Science 223:1265–1268.
Williams, C.E., Kloeckener-Guissem, B., Freeling, M. 1991. Naturally occurring variants of maize Adh1 differ in organ-specific expression both in quantity and developmental timing. Maydica 36:115–128.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
About this article
Cite this article
Hajós-Novák, M., Dallmann, G. & Kristóf, Z. Transposon-derived organ-specific mutation in the ADH1 RNA 5′ untranslated leader of autotetraploid maize (Zea mays L.). CEREAL RESEARCH COMMUNICATIONS 37, 151–158 (2009). https://doi.org/10.1556/CRC.37.2009.2.1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1556/CRC.37.2009.2.1