Abstract
Transposon mutagenesis was applied to generate mutants in Fusarium graminearum. The mimp1/impala system originally identified in F. oxysporum proved very promising for mutagenesis as the transposon and reinserted at high frequency in (the vicinity) of genes. A collection of mutants was screened for growth, for pathogenicity and for perithecia production. Several mutants blocked in one or more functions were obtained. The wild-type phenotype of one such mutant could be restored by complementation with a non-disrupted copy of the gene. In addition reinsertions occurred on each of the four chromosomes of F. graminearum, making this system a powerful tool in the functional analyses of the > 10,000 genes predicted in the F. graminearum genome.
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Dufresne, M., Van Der Lee, T., Ben M’Barek, S. et al. Tagging pathogenicity genes in Fusarium graminearum using the transposon system mimp/impala. CEREAL RESEARCH COMMUNICATIONS 36 (Suppl 6), 415–419 (2008). https://doi.org/10.1556/CRC.36.2008.Suppl.B.34
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DOI: https://doi.org/10.1556/CRC.36.2008.Suppl.B.34