Summary/Abstract
The aim of the experiments was to develop translocation lines by inducing homoeologous chromosome pairing in a 4H(4D) wheat-barley substitution line previously developed in Martonvásár. It was hoped to incorporate various segments of the barley 4H chromosome from the 4H(4D) substitution into wheat. Observations were made on the frequency with which wheat-barley translocations appeared in the F2 progeny grains from a cross between the line CO4-1, which carries the Ph suppressor gene from Aegilops speltoides and thus induces a high level of homoeologous chromosome pairing, and the 4H(4D) wheat-barley substitution line, and on which chromosome segments were involved in the translocations. The translocations were identified by means of genomic in situ hybridisation. Of the 117 plants examined, three (2.4 %) were found to contain translocations. A total of four translocations were observed, as one plant contained two different translocations. The translocations consisted of one centric fusion, two dicentric translocations and one acrocentric chromosome. Plants carrying translocations were raised in the phytotron and the selection of homozygous translocation lines was commenced from the F3 progeny grains.
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30 December 2007
The following supplement should have been added to the paper by Sepsi et al.: “Induction of Chromosome Rearrangements in a 4H(4D) Wheat-Barley Substitution Using a Wheat Line Containing a Ph Suppressor Gene” published in Cereal Research Communications 34(4):1215–1222.
References
Chen, P.D. — Tsujimoto, H. — Gill, B.S.: 1994. Transfer of Ph1 genes promoting homoeologous pairing from Triticum speltoides to common wheat. Theor. Appl. Genet., 88:97–101.
Devos, K. M. — Gale, M. D.: 2000. Genome relationships: the grass model in current research. Plant Cell, 12: 637–646.
Dvorak, J.: 1972. Genetic variability in Aegilops speltoides affecting homoeologous pairing in wheat. Can. J. Cytol., 14: 371–380.
Fedak, G.: 1997. Increased homoeologous chromosome pairing in Hordeum vulgare × Triticum aestivum hybrids. Nature, 266: 529–530.
Feldman, M.: 1988. Cytogenetic and molecular approaches to alien gene transfer in wheat. In: Miller, T.E., Koebner, R.M.D. (eds.), Proc. 7th International Wheat Genetics Symposium, Cambridge, pp 23–32.
Friebe, B. — Jiang, J. — Raupp, W. J. — McIntosh, R.A. — Gill, B.S.: 1996. Characterization of wheat-alien translocations conferring resistance to diseases and pests: current status. Euphytica, 91: 59–87.
Islam, A. K. M. R. — Shepherd, K.W. — Sparrow, D. H. B.: 1978. Production and characterization of wheat-barley addition lines. In: S. Ramunujam (ed.), Proc. 5th Int. Wheat Genet. Symp., New Delhi, India, pp. 356–371.
Islam, A. K. M. R. — Shepherd, K. W. — Sparrow, D. H. B.: 1981. Isolation and characterization of euplasmic wheat-barley chromosome addition lines. Heredity, 46: 160–174.
Islam, A. K. M. R. — Shepherd, K. W.: 1992. Production of wheat-barley recombinant chromosomes through induced homoeologous pairing. 1. Isolation of recombinants involving barley arms 3HL and 6HL. Theor. Appl. Genet., 83: 489–494.
Jauhar, P. P. — Chibbar, R. N.: 1999. Chromosome-mediated and direct gene transfers in wheat. Genome, 42: 570–583.
Kruse, A.: 1973. Hordeum × Triticum hybrids. Hereditas, 73: 157–161.
Kreis, M. — Williamson, M. S. — Shewry, P. R. — Sharp, P. — Gale, M.: 1988. Identification of a second locus encoding β-amylase on chromosome 2 of barley. Genet. Res., Camb., 51: 13–16.
Le, H. T. — Armstrong, K. C. — Miki, B.: 1989. Detection of rye DNA in wheat-rye hybrids and wheat translocation stocks using total genomic DNA as a probe. Plant Mol. Biol. Rep., 7: 150–158.
Linc, G. — Friebe, B. — Kynast, R. G. — Molnár-Láng, M. — Kőszegi, B. — Sutka, J. — Gill, B. S.: 1999. Molecular cytogenetic analysis of Aegilops cylindrica. Host. Genome, 42: 497–503.
Linc, G. — Molnár-Láng, M.: 2003. Producing new wheat/barley disomic additions in winter wheat background using different methods and analyse them by molecular cytogenetic techniques Növénytermelés, 52:3–13. (Summary in English).
Molnar, I.-Linc, G.-Dulai, S.-D.Nagy, E.-Molnár-Láng, M.: 2005. Ability of chromosome 4H to compensate for 4D in response to drought stress in a newly developed wheat-barley 4H(4D) disomic substitution line. Submitted to Plant Breeding
Molnár-Láng, M. — Sutka, J. — Barnabás, B. — Sági, L. — Belea, A.: 1985. Production of barley (Hordeum vulgare) ×wheat (Triticum aestivum L.). Növénytermelés, 34: 257–261. (Summary in English).
Molnár-Láng, M. — Sutka, J.: 1994. The effect of temperature on seed set and embryo development in reciprocal crosses of wheat and barley. Euphytica, 78: 53–58.
Molnár-Láng, M. — Linc, G. — Friebe, B. R. — Sutka, J.: 2000. Detection of wheat-barley translocations by genomic in situ hybridization in derivatives of hybrids multiplied in vitro. Euphytica, 112: 117–123.
Reader, S. M. — Abbo, S. — Purdie, K. A. — King, I. P. — Miller, T. E.: 1994. Direct labelling of plant chromosomes by rapid in situ hybridization. Trends Genet., 10: 265–266.
Riley, R.-Chapman V.: 1958. Genetic control of the cytologically diploid behaviour of hexaploid wheat. Nature, 182:713–715.
Riley, R. — Kimber, G. — Chapman, V.: 1961. Origin of genetic control of diploid-like behavior of polyploid wheat. J. Hered., 52: 22–25.
Riley, R. — Kempana, C.: 1963. The homoeologous nature of the non-homologous meiotic pairing in Triticum aestivum deficient for chromosome V (5B). Hered., 18: 287–306.
Riley, R. — Chapman, V. — Johnson, R.: 1968. The incorporation of alien disease resistance in wheat by genetic interference with the regulation of meiotic chromosome synapsis. Genet. Res., Camb., 12: 199–213
Schwarzacher, T. — Leitch, A. R. — Bennett, M. D. — Heslop-Harrison, J. S.: 1989. In-situ localization of parental genomes in a wide hybrid. Ann. Bot., London, 64: 315–324.
Schwarzacher, T. — Anamthawat-Jónsson, K. — Harrison, G. E. — Islam, A. K. M. R. — Jia, J. Z. — King, I. P. — Leitch, A. R. — Miller, T. E. — Reader, S. M. — Rogers, W. J. — Shi, M. — Heslop-Harrison, J. S.: 1992. Genomic in situ hybridization to identify alien chromosomes and chromosome segments in wheat. Theor. Appl. Genet., 84: 778–786.
Sears, E. R.: 1952. Homoeologous chromosomes in Triticum aestivum. Genet. 37: 624.
Sears, E. R. — Okamoto, M.: 1958. Intergenomic chromosome relationships in hexaploid wheat. Proc. 10th Int. Cong. Genet., 2: 258–259.
Sears, E. R.: 1973. Agropyron-Wheat transfers induced by homoeologous pairing. Proc. 4th Int. Wheat Genet. Symp., Columbia, 191–200.
Sears, E. R.: 1977. An induced mutant with homoeologous pairing in common wheat. Can. J. Genet. Cytol., 19: 585–593.
Sears, E. R. — Loegering, W.Q.: 1982. Elimination of an undesirable trait from an alien translocation line by induced homoeologous pairing. In: Singh RB, Singh RM and Singh BD, (eds), Advances in Cytogenetics and Crop Improvement, Kalyani publishers, New Delhi, pp. 81–86.
Schubert, L. — Shi, F. — Fuchs, J. — Endo, T. R.: 1998. An efficient screening for terminal deletions and translocations of barley chromosomes added to common wheat. Plant J., 14: 489–495.
Sherman, J. D. — Smith, L. Y. — Blake, T. K. — Talbert, L. E.: 2001. Identification of barley genome segments introgressed into wheat using PCR markers. Genome, 44: 38–44.
Taketa, S. — Awayama, T. — Ichii, M. — Sunakawa, M. — Kawahara, T. — Murai, K.: 2005. Molecular cytogenetic identification of nullisomy 5B induced homoeologous recombination between wheat chromosome 5D and barley chromosome 5H. Genome, 48: 115–124.
Zhu, B. — Choi, D. W. — Fenton, R. — Close, T.J.: 2000. Expression of the barley dehydrin multigene family and the development of freezing tolerance. Mol. Gen. Genet., 264: 145–53.
Zitzewitz, J. — Szucs, P. — Dubcovsky, J. — Yan, L. — Francia, E. — Pecchioni, N. — Casas, A. — Chen, T. H. — Hayes, P. M. — Skinner, J. S.: 2005. Molecular and structural characterization of barley vernalization genes. Plant. Mol. Biol., 59(3):449–67.
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Sepsi, A., Németh, K., Molnár, I. et al. Induction of chromosome rearrangements in a 4H(4D) wheat-barley substitution using a wheat line containing a Ph suppressor gene. CEREAL RESEARCH COMMUNICATIONS 34, 1215–1222 (2006). https://doi.org/10.1556/CRC.34.2006.4.261
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DOI: https://doi.org/10.1556/CRC.34.2006.4.261