Summary
The effectiveness of Cu 2+ accumulation was investigated in three wheat cultivars with different copper sensitivity (Triticum aestivum cv. GK Tiszatáj, GK Kata and GK Öthalom). Supraoptimal Cu 2+ concentrations result in toxicity symptoms in the sensitive genotype and increase the production of the stress hormone, ethylene both in the leaves and root tissues of wheat seedlings. The sensitive cultivar, cv. Öthalom produced less ethylene than the tolerant genotypes (cvs Tiszatáj and Kata) in the roots whether the ethylene measurements were done over the 6-h period after Cu 2+ exposure. Levels of 1-aminocyclopropane-1-carboxylic acid (ACC), the immediate precursor of ethylene, did not change characteristically during this period in either the tolerant or sensitive seedlings. The biosynthesis of ethylene has a common intermediate, S-adenosylmethionine (SAM) with the synthesis of the polyamine spermidine and 2-deoxymugineic acid, a Fe 3+ -solubilizing and transporting wheat phytosiderophore. These chelating substances also mediate the transport of different bivalent cations, such as Cu 2+. The biosynthetic pathways of ethylene and polyamines, spermidine and spermine may compete for SAM with the phytosiderophore synthesis. Simultaneous inhibition of SAM decarboxylase by 0.5 M methylglyoxal bis (guanylhydrazone) (MGBG), and 1-aminocyclopropane-1-carboxylic acid synthase by 10 μM (2-aminoethoxy-vinyl)glycine (AVG), significantly increased the Cu 2+ accumulation in root tissues of the wheat cultivars independently of their sensitivities. MGBG alone resulted in an enhanced copper content but AVG proved to be ineffective. This suggests that the amount of SAM allocated for polyamine formation may limit the phytosiderophore synthesis or spermidine (spermine) in itself may control the uptake of Cu 2+.
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References
Bregoli, A.M., Scaramagli, S., Costa, G., Sabatini, E., Ziosi, V., Biondi, S., Torrigiani, P. (2002) Peach (Prunus persica) fruit ripening: Aminoethoxyvinylglycine (AVG) and exogenous polyamines affect ethylene emission and flesh firmness. Physiol. Plant. 114: 472–481.
Ciscato, M., Valcke, R., Van Loven K., Clijsters, H., Navari-Izzo, F. (1997) Effects of in vivo copper treatment on the photosynthetic apparatus of two Triticum durum cultivars with different sensitivity. Physiol. Plant. 100: 901–908.
Demidchik, V., Sokolik, A., Yurin, V. (1997) The effect of Cu 2+ on ion transport systems of the plant cell plasmalemma. Plant Physiol. 114: 1313–1325.
De Vos, C.H.R., Schat, H. (1991) Free radicals and heavy metal tolerance. — In: Rozema, J., Verkleij, J.A.C. (eds): Ecological responses to environmental stresses. Pp. 22–33. Kluwer Academic Publishers, Dordrecht.
Even-Chen, Z., Mattoo, A.K., Goren, R. (1982) Inhibition of ethylene biosynthesis by aminoethoxyvinylglycine and by polyamines shunts label from 3,4-[14 C-methionine] into spermidine in aged orange peel discs. Plant Physiol. 69: 385–388.
Ievinsh, G., Valcina, A., Ozola, D. (1995) Induction of ascorbate peroxidase activity in stressed pine (Pinus sylvestris L.) needles: a putative role for ethylene. Plant Sci. 112: 167–173.
Jiang, W., Liu, D., Liu, X. (2001) Effects of copper on root growth, cell division, and nucleolus of Zea mays. Biol. Plant. 44: 105–109.
Li, N., Parsons, B., Liu, D., Mattoo, A.K. (1992) Accumulation of wound-inducible ACC synthase transcript in tomato fruit is inhibited by salicylic acid and polyamines. Plant Mol. Biol. 18: 477–487.
Ma, J.F., Taketa, Sh., Chang, Y-Ch., Takeda, K., Matsumoto, H. (1999) Biosynthesis of phytosiderophores is several Triticeae species with different genomes. J. Exp. Bot. 50: 723–726.
Mader, J. C. (2004) Differential in vitro development of inflorescences in long and short day Lemna spp.: Involvement of ethylene and polyamines. J. Plant Physiol. 161: 653–663.
Marrs, K.A. (1996) The functions and regulation of glutathione S-transferases in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol. 47: 127–158.
Mori, S, Nishizawa, N. (1987) Methionine as a dominant precursor of phytosiderophores in Gramineae plants. Plant Cell Physiol. 28: 1081–1092.
Quan, Y., Minocha, R., Minocha, S.C. (2002) Genetic manipulation of polyamine metabolism in poplar II: effects on ethylene biosynthesis. Plant Physiol. Biochem. 40: 929–937.
Shenker, M., Fan, T.W.-M., Crowley, D.E. (2001) Phytosiderophores influence on cadmium mobilization and uptake by wheat and barley plants. J. Environ. Qual. 30: 2091–2098.
Tari, I., Mihalik, E. (1998) Comparison of the effects of white light and the growth retardant paclobutrazol on the ethylene production in bean hypocotyls. Plant Growth Regul. 24: 67–72.
Tari, I, Szalai, G., Lőrincz, Zs., Bálint, A. (2002) Changes in thiol content in roots of wheat cultivars exposed to copper stress. Biol. Plant. 45: 255–260.
Turano, F.J., Kramer, G.F., Wang, C.Y. (1997) The effect of methionine, ethylene and polyamine catabolic intermediates on polyamine accumulation in detached soybean leaves. Physiol. Plant. 101: 510–518.
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Tari, I., Csiszár, J., Gémes, K. et al. Modulation of Cu 2+ accumulation by (aminoethoxyvinyl)glycine and methylglyoxal bis (guanylhydrazone), the inhibitors of stress ethylene and polyamine synthesis in wheat genotypes. CEREAL RESEARCH COMMUNICATIONS 34, 989–996 (2006). https://doi.org/10.1556/CRC.34.2006.2-3.229
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DOI: https://doi.org/10.1556/CRC.34.2006.2-3.229