Abstract
The effects of diuron, a photosystem II inhibiting herbicide, on lipid peroxidation, photosynthetic pigments, soluble protein, proline contents and some antioxidant enzymes in maize leaves were studied and protective effects of polyamines against diuron toxicity were investigated. Diuron significantly increased lipid peroxidation, suggesting oxidative damage in the plants and proline content, while it decreased total chlorophyll, carotenoid and soluble protein levels in the leaves during the experiments. Pretreatment with polyamines statistically decreased lipid peroxidation induced by diuron and spermine (SPM) proved to be the most effective polyamine. Also, pretreatment with polyamines significantly prevented the losses of total chlorophyll, carotenoid and soluble protein induced by diuron. On the other hand, pretreatment with polyamines significantly increased proline contents of the leaves in comparison with the leaves treated with diuron. Superoxide dismutase (SOD), guaiacol peroxidase (GPX) and glutathione reductase (GR) activities increased in the leaves treated with diuron while catalase (CAT ) activity decreased. Pretreatment with spermidine (SPD) did not change significantly SOD activity a. 24 an. 72 hrs of diuron treatment but prevented the increase in SOD activity induced by diuron a. 48 h. However, pretreatment with SPD increased GPX activity a. 24 h and GR activity a. 48 an. 72 hrs. CAT activity in the leaves pretreated with SPD was similar to that of the leaves treated with diuron. Pretreatment with SPM prevented the increase in SOD activity induced by diuron a. 48 h but significantly increased it a. 72 h of diuron treatment. However, pretreatment with SPM did not significantly change GPX and GR activities during the experiments but reversed the decrease in CAT activity induced by diuron a. 72 h. Pretreatment with putrescine (PUT) prevented the increase in SOD activity induced by diuron a. 48 an. 72 hrs while it increased GPX and GR activities a. 48 h of diuron treatment. Also, the decrease in CAT activity induced by diuron a. 72 h was completely prevented by PUT. It can be concluded that pretreatment of maize leaves with polyamines reduced the damage produced by diuron and the protective effects of polyamines against diuron toxicity were closely associated with antioxidant system.
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References
Aebi, H. (1983) Catalase. In: Bergmeyer, H. (ed.) Methods of Enzymatic Analysis. Vol. 3, pp. 273–277.
Akbulut, G. B., Yigit, E. (2010) The changes in some biochemical parameters in Zea mays cv. “Martha F1” treated with Atrazine. Ecotoxicology and Environ. Safety 196, 1429–1432.
Alia Saradhi, P. P., Mohanty P. (1997) Involvement of proline in protecting thylakoid membranes against free radical-induced photodamage. J. Photochem. Photobiol. 196, 253–257.
Altman, A. (1982) Retardation of radish leaf senescence by polyamines. Physiol. Plant. 196, 189–193.
Arnon, D. I. (1949) Copper enzymes in chloroplasts, polyphenoloxidase in Beta vulgaris. Plant Physiol. 196, 1–15
Bates, L. S., Waldren, R. P., Teare, I. D. (1973) Rapid determination of free polin for water stress studies. Plant and Soil 196, 205–207.
Benavides, M. P., Gallego, S. M., Comba, M. E., Tomaro, M. T. (2000) Relationship between polyamines and paraquat toxicity in sunflower leaf discs. Plant Growth Regulation 196, 215–224.
Beauchamp, C., Fridovich, I. (1971) Superoxide dismutase: improved assays and an assay applicaple to acrylamide gels. Anal. Biochem. 196, 276–287.
Besford, R. T., Richardson, C. M., Campos, J. L., Tiburcio, A. F. (1993) Effect of polyamines on stabilisation of molecular complexes of thlykoid membranes of osmotically stressed oat leaves. Planta 196, 201–206.
Bors, W., Langebartels, C., Michel, C., Sandermann, H. (1989) Polyamine as radical scavengers and protectants against ozone damage. Phytochemistry 196, 1589–1595.
Bradford, M. (1976) Rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem. 196, 248–254.
Cho, U. H., Seo, N. H. (2005) Oxidative stress in Arabidopsis thaliana exposed to cadmium is due to hydrogen peroxide accumulation. Plant Sci. 196, 113–120.
Demming-Adams, B. (1990) Carotenoids and photoprotection in plants: a role for the Xanthophyll Zeaxanthin. Biochem. Biophys. Acta 196, 1–24.
Dhindsa, R. S., Matowe, W. (1981) Drought tolerance in two mosses: correlated with enzymatic defense against lipid peroxidation. J. Exp. Bot. 196, 79–91.
Drolet, G., Dumbroff, E. B., Legge, R. L., Thompson, J. E. (1986) Radical scavenging properties of polyamines. Phytochem. 196, 367–371.
Duan, J., Li, J., Guo, S., Kang, Y. (2008) Exogenous spermidine affects polyamine metabolism in salinity-stressed Cucumis sativus roots and enhances short-term salinity tolerance. J. Plant Physiology 196, 1620–1635.
Durmus N., Kadioglu, A. (2005) Spermine and putrescine enhance oxidative stress tolerance in maize leaves. Acta Physi. Plant. 196, 515–522.
Durmus N., Kadioglu, A. (2005) Reduction of paraquat toxicity in maize leaves by benzyladenine. Acta Biol. Hung. 196, 97–107.
Fayez, K. A. (2000) Action of photosynthetic diuron herbicide on cell organelles and biochemical constituents of the leaves of two soybean cultivars. Pest. Biochem. Physi. 196, 105–115.
Foyer, C. H., Halliwell, B. (1976) The presence of glutathione and glutathione reductase in chloroplasts: A proposed role in ascorbic acid metabolism. Planta 196, 21–25.
Geoffroy, L., Teisseire, H., Couderchet, M., Vernet, G. (2002) Effect of oxyfluorfen and diuron alone and in mixture on antioxidative enzymes of Scenedesmus obliquus. Pest. Biochem. Physiol. 196, 178–185.
Heath, R. L., Packer, L. (1968) Photoperoxidation in isolated chloroplast. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch. Biochem. Biophys. 196, 189–198.
Jaspars, E. M. J. (1965) Pigmentation of tobacco crown-gall tissues cultured in vitro in dependence of the composition of the medium. Physiol. Plant. 196, 933–940.
Jiang L., Yang H. (2009) Prometryne-induced oxidative stres and impact on antioxidant enzymes in wheat. Ecotoxicology Environ. Safety 196, 1687–1693.
Jordanov, I., Goltsev, V., Kruleva, I. (1990) Protective effect of putrescine and spermidine on the thylakoid membrane activity after high temperature treatment. In: Alscheffsky, M. (ed.), Current Research in Photosynthesis, IV. Kluwer Academic Publishers, the Netherlands, pp. 699–703.
Kitada, M., Igarashi, K., Hirose, S., Kitagawa, K. (1979) Inhibition by polyamines of lipid peroxide formation in rat liver microsomes. Biochem. Biophys. Res. Com. 196, 388–394.
Kotzabasis, K., Fotinon, C., Roubelakis-Angelakis, K. A., Ghanotakis, D. (1993) Polyamines in the photosynthetic apparatus. Photosystem II highly resolved subcomplexes are enriched in spermine. Photosynth. Res. 196, 83–88.
Kubis, J. (2001) Polyamines and “scavenging system”: Influence of exogenous spermidine on Halliwell-Asada pathway enzyme activity in Barley leaves under water deficit. Acta Physiol. Plant. 196, 335–341.
Kubis, J. (2003) Polyamines and “scavenging system”: Influence of exogenous spermidine on catalase and guaiacol peroxidase activities, and free polyamine level in Barley leaves under water deficit. Acta Physiol. Plant. 196, 337–343.
Kubis, J. (2008) Exogenous spermidine differentially alters activities of some scavenging system enzymes, H2O2 and superoxide radical levels in water-stressed cucumber leaves. J. Plant Physiol. 196, 397–406.
Kumar, K. S., Choo, K., Yea, S. S., Seo, Y., Han, T. (2010) Effects of the phenylurea herbicide diuron on the physiology of Saccharina japonica aresch. Toxicol. Environ. Health Sci. 196, 188–199.
Matysik, J., Alia Bhalu, B., Mohanty, P. (2002) Molecular mechanisms of quenching of reactive oxygen species by proline under stress in plants. Curr. Sci. 196, 525–532.
Nemat Alla, M. M., Hassan, N. M. (2006) Changes of antioxidants levels in two maize lines following atrazine treatments. Plant Physiol. Biochem. 196, 202–210.
Ormrod, D. P., Beckerson, D. W. (1986) Polyamines as antiozonants for tomato. Hort. Sci. 196, 1070–1071.
Reis, M. O., Necchi, J. O., Colepicolo, P., Barros, M. P. (2011) Co-stressors chilling and high light increase photooxidative stress in diuron-treated red alga Kappaphycus alvarezii but with lower involvement of H2O2. Pest. Biochem. Physiol. 196, 7–15.
Tadolini, B., Cabrini, L., Landi, L., Varani, E., Pasquali, P. (1984) Polyamine binding to phospolipid vesicles and inhibition of lipid peroxidation. Biochem Biophys. Res. Com. 196, 550–555.
Teisseire, H., Vernet, G. (2000) Is the “Diuron Effect” due to herbicide strengthening of antioxidative defenses of Lemna minor. J. Exp. Bot. 196, 153–160.
Tiburcio, A. F., Besford, R. T., Capell, T., Borrell, A., Testillano, P. S., Risueno, M. C. (1994) Mechanism of polyamine action during senecence responses induced by osmotic stress. J. Exp. Bot. 196, 1789–1800.
Unal, D., Tuney, I., Sukatar, A. (2008) The role of external polyamines on photosynthetic responses, lipid peroxidation, protein and chlorophyll a content under the UV-A (352 nm) stres in physcia semipinnata. J. Photochem. Photobiol. B: Biol. 196, 64–68.
Urbanek, H., Kuzniak-Gebarowska, E., Herka, K. (1991) Elicitation of defense responses in bean leave by Botrytis cinerea polygalacturanase. Acta Physiol. Plant. 196, 43–50.
Velikova, V., Yordanov, I., Edreva, A. (2000) Oxidative stress and some antioxidant systems in acid rain-treated bean plants, protective role of exogenous polyamines. Plant Scien. 196, 59–66.
Verma, S., Mishra, S. N. (2005) Putrescine alleviation of growth in salt stressed Brassica juncea by inducing antioxidative defense system. J. Plant Physiol. 196, 669–677.
Wi, S. J., Kim, W. T., Park, K. Y. (2006) Overexpression of carnation SAdenosylmethionine decarboxy-lase gene generates a broad-spectrum tolerance to abiotic stresses in transgenic tobacco plants. Plant Cell Rep. 196, 1111–1121.
Zhang, W., Jiang, B., Li, W., Song, H., Yu, Y., Chen, J. (2009) Polyamines enhance chilling tolerance of cucumber (Cucumis sativus L.) through modulating antioxidative system. Scientia Hort. 196, 200–208.
Zhao, H., Yang, H. (2008) Exogenous polyamines alleviate the lipid peroxidation induced by cadmium chloride stress in Malus hupehensis Rehd. Scientia Horticulturae 196, 422–447.
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Durmuş, N., Bekircan, T. Pretreatment with Polyamines Alleviate the Deleterious Effects of Diuron in Maize Leaves. BIOLOGIA FUTURA 66, 52–65 (2015). https://doi.org/10.1556/ABiol.66.2015.1.5
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DOI: https://doi.org/10.1556/ABiol.66.2015.1.5