Abstract
The one-gene mutation in the tert-butyl hydroperoxide-resistant mutant hyd1-190 of the fission yeast Schizosaccharomyces pombe led to a 4-fold increase in resistance to t-BuOOH and decreased specific concentrations of superoxide and total thiols in comparison with the parental strain hyd+. It suggested an unbalanced redox state of the cells, which induced continuously increased specific activities of glutathione peroxidase, glutathione reductase and glutathione S-transferase and decreased activities of the antioxidant enzymes superoxide dismutases and glucose-6-phosphate dehydrogenase to regulate the redox balance of the mutation-induced permanent, low-level but tolerable internal stress. These results may contribute to the understanding of internal, oxidative stress-related human diseases.
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Abbreviations
- CAT:
-
catalase
- C. albicans:
-
Candida albicans
- EPR:
-
electron paramagnetic resonance spectroscopy
- G6PD:
-
glucose-6-phosphate dehydrogenase
- GSH:
-
glutathione
- GSG:
-
oxidized glutathione
- GR:
-
glutathione reductase
- GPx:
-
glutathione peroxidase
- GST:
-
glutathione S-transferase
- H2O2:
-
hydrogen peroxide
- O2•–:
-
superoxide anion
- •OH:
-
hydroxyl radical
- PBN:
-
N-tert-butyl-α-phenylnitrone
- ROS:
-
reactive oxygen species
- S. cerevisiae:
-
Saccharomyces cerevisiae
- S. pombe:
-
Schizosaccharomyces pombe
- SODs:
-
superoxide dismutases
- t-BuOOH:
-
tert-butyl hydroperoxide
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Gazdag, Z., Kálmán, N., Blaskó, Á. et al. Regulation of the Unbalanced Redox State in a Schizosaccharomyces Pombe tert-Butyl Hydroperoxide-Resistant Mutant. BIOLOGIA FUTURA 65, 218–226 (2014). https://doi.org/10.1556/ABiol.65.2014.2.9
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DOI: https://doi.org/10.1556/ABiol.65.2014.2.9