Abstract
Responses of parameters related with photosynthesis and the involvement of various factors in photosynthetic damage in two chickpea genotypes, Gokce (tolerant) and Kusmen (sensitive) under drought stress were assessed. Photosynthetic pigment content decreased under drought stress in two genotypes. Signifcant decreases in gs, Pn and E were determined in Kusmen. No signifcant change in these parameters was measured in Gokce under drought stress. Fv/Fm, ΦPS2 and ETR decreased in drought stressed plants of Kusmen as compared to control plants however Fv/Fm, ΦPS2 and ETR did not change in Gokce under drought stress. Increases in NPQ were determined under stress in both genotypes. Drought stress did not affect rubisco activity and rubisco concentration in Gokce while, the activity and the content declined in Kusmen. The drought tolerance of the Gokce genotype is a consequence of a balance among leaf water potential, stomatal conductance, photosynthesis, and transpiration. On the other hand, photosynthesis in Kusmen may be not only restricted by stomatal limitations but also by non-stomatal limitations under drought stress.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- ΦPS2:
-
Effective quantum yield of PS2 photochemistry
- Fm:
-
Maximal fuorescence yield in dark-adapted state
- Fm’:
-
Maximum Chl fuorescence yield in the light adapted state
- Fv/Fm:
-
Maximum quan¬tum yield of PS2 photochemistry
- Fo:
-
Minimal fuorescence yield in dark-adapted state
- Pn:
-
Net photosynthetic rate
- NPQ:
-
Non-photochemical quenching
- gs:
-
Stomatal conductance
- E:
-
Transpiration
References
Ahmed, S., Nawata, E., Hosokawa, M., Domae, Y., Sakuratani, T. (2002) Alterations in photosynthesis and some antioxidant enzymatic activities of mungbean subjected to waterlogging. Plant Sci. 163, 117–123.
Anonymous (2000) Turkish Republic Ministry of Agriculture and Rural Affairs. General Directorate of Agricultural Research, Center of Crop Plants Research Institute, Species Catalogue, Ankara, 31 p.
Arnon, D. (1949) Copper enzymes in isolated chloroplasts: polyphenol oxidases in Beta vulgaris. Plant Physiol. 24, 1–15.
Bayoumi, T. Y., Eid, M., Metwali, E. M. (2008) Application of physiological and biochemical indices as a screening technique for drought tolerance in wheat genotypes. Afr. J. Biotechnol. 7, 2341–2352.
Blum, A., Ebercon, A. (1981) Cell membrane stability as a measure of drought and heat tolerance in wheat. Crop Sci. 21, 43–47.
Bradford, M. M. (1976) Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254.
Dulai, S., Molnar, I., Pronay, J., Csernak, A., Tarnai, R., Molnar Lang, M. (2006) Effects of drought on photosynthetic parameters and heat stability of PSII in wheat and in Aegilops species originating from dry habitats. Acta Biol. Szeged. 50, 11–17.
Duzdemir, O. (2011) Stability analysis for phenological characteristics in chickpea. Afr. J. Agric. Res. 6, 1682–1685.
El Solh, M. (2011) Enhancing Food Security Under Water Scarcity: The Role of Science and Technology. The 2nd Arab Water Forum, Cairo, Egypt, 20–23 November, 2011.
Genty, B., Briantais, J. M., Baker, N. R. (1989) The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochim. Biophys. Acta 990, 87–92.
Güneş, A., Inal, A., Adak, M. S., Bagci, E. G., Cicek, N., Eraslan, F. (2008) Effect of drought stress implemented at pre- or postanthesis stage on some physiological parameters as screening criteria in chickpea cultivars. Russ. J. Plant Physiol. 55, 59–67.
Jaspars, E. M. J. (1965) Pigmentation of tobacco crown-gall tissues cultured in vitro in dependence of the composition of the medium. Physiol. Plant. 18, 933–940.
Khayatnezhad, M., Gholamin, R. (2012) The effect of drought stress on leaf chlorophyll content and stress resistance in maize cultivars (Zea mays). Afr. J. Microbiol. Res. 6, 2844–2848.
Kholová, J., Hash, C. T., Kakkera, A., Kočová, M., Vadez, V. (2010) Constitutive water conserving mechanisms are correlated with the terminal drought tolerance of pearl millet (Pennisetum glaucum (L.) R. Br.). J. Exp. Bot. 61, 369–377.
Kholová, J., Hash, C. T., Lava Kumar P., Yadav, R. S., Kočová, M., Vadez, V. (2010) Terminal drought-tolerant pearl millet (Pennisetum glaucum (L.) R. Br.) have high leaf ABA and limit transpiration at high vapour pressure deficit. J. Exp. Bot. 61, 1431–1440.
Krouma, A. (2010) Plant water relations and photosynthetic activity in three Tunisian chickpea (Cicer arietinum L.) genotypes subjected to drought. Turk. J. Agric. For. 34, 257–264.
Lal, A., Ku, M. S. B., Edwards, G. E. (1996) Analysis of inhibition of photosynthesis due to water stress in C3 species Hordeum vulgare and Vicia faba: Electron transport, CO2 fixation and carboxylation capacity. Photosynth. Res. 49, 57–69.
Liu, C., Liu, Y., Guo, K., Fan, D., Li, G., Zheng, Y., Yu, L., Yang, R. (2011) Effect of drought on pigments, osmotic adjustment and antioxidant enzymes in six woody plant species in karst habitats of southwestern China. Environ. Exp. Bot. 71, 174–183.
Ma, Z., Cooper, C., Kim, H. J., Janick-Buckner, D. (2009) A study of rubisco through western blotting and tissue printing techniques. CBE Life Sci. Educ. 8, 140–146.
Macar, K. T., Ekmekci, Y. (2008) PSII photochemistry and antioxidant responses of a chickpea variety exposed to drought. Z. Naturforsch. C 63, 583–594.
Macar, K. T., Ekmekci, Y. (2009) Alterations in photochemical and physiological activities of chickpea (Cicer arietinum L.) cultivars under drought stress. J. Agron. Crop Sci. 195, 335–346.
Mafakheri, A., Siosemardeh, A., Bahramnejad, B., Struik, P. C., Sohrabi, E. (2010) Effect of drought stress on yield, proline and chlorophyll contents in three chickpea cultivars. Aust. J. Crop Sci. 4, 580–585.
Marques da Silva, J., Arrabica, M. C. (1995) Effect of water stress on Rubisco activity of Setaria sphacelota. In: Mathis, P. (ed.) Photosynthesis: From Light to Biosphere. Kluwer Academic Publishers, London, pp. 545–548.
Mishra, K. B., Iannacone, R., Petrozza, A., Mishra, A., Armentano, N., La Vecchia, G., Trtílek, M., Cellini, F., Nedbal, L. (2012) Engineered drought tolerance in tomato plants is reflected in chlorophyll fluorescence emission. Plant Sci. 182, 79–86.
Nar, H., Saglam, A., Terzi, R., Varkonyi, Z., Kadioglu, A. (2009) Leaf rolling and photosystem II. Efficiency in Ctenanthe setosa exposed to drought stress. Photosynthetica 47, 429–436.
Pouresmael, M., Khavari-Nejad, R. A., Mozafari, J., Najafi, F., Moradi, F. (2013) Efficiency of screening criteria for drought tolerance in chickpea. Arch. Agron. Soil Sci. 59, 1675–1693.
Rahbarian, R., Khavari-Nejad, R., Ganjeali, A., Bagheri, A., Najafi, F. (2011) Drought stress effects on photosynthesis, chlorophyll fluorescence and water relations in tolerant and susceptible chickpea (Cicer arietinum L.) genotypes. Acta Biol. Cracov. Bot. 53, 47–55.
Roháček, K., Soukupová, J., Barták, M. (2008) Chlorophyll fluorescence: A wonderful tool to study plant physiology and plant stress. In: Schoefs, B. (ed.) Plant Cell Compartments–Selected Topics. Research Signpost, India, pp. 41–104.
Sairam, P. K., Deshmukh, P. S., Shukla, D. S. (1997) Tolerance of drought and temperature stress in relation to increased antioxidant enzyme activity in wheat. J. Agron. Crop Sci. 178, 171–178.
Sawada, S. S., Sato, M., Kasai, A., Yaochi, D., Kameya, Y., Matsumoto, L., Kasai, D. (2003) Analysis of the feed-forward effects of sink activity on the photosynthetic source-sink balance in single-rooted sweet potato leaves. I. Activation of RuBPcase through the development of sinks. Plant Cell Physiol. 44, 190–197.
Siddique, M. R. B., Hamid, A., Islam, M. S. (2000) Drought stress effects on water relations of wheat. Bot. Bull. Acad. Sin. 41, 35–39.
Singh, R., Sharma, P., Varshney, R. K., Sharma, S. K., Singh, N. K. (2008) Chickpea improvement: Role of wild species and genetic markers. Biotechnol. Genet. Eng. 25, 267–314.
Slavick, B. (1974) Methods of Studying Plant Water Relations. Springer-Verlag, Berlin, p. 449.
Xu, Z. Z., Zhou, G. S., Wang, Y. L., Han, G. X., Li, Y. J. (2008) Changes in chlorophyll fluorescence in maize plants with imposed rapid dehydration at different leaf ages. J. Plant Growth Regul. 27, 83–92.
Yordanov, I., Tsonev, T., Velikova, V., Georgieva, K., Ivanov, P., Tsenov, N., Petrova, T. (2001) Changes in CO2 assimilation, transpiration and stomatal resistance of different wheat cultivars experiencing drought under field conditions. Bulg. J. Plant Physiol. 27, 20–33.
Zaman-Allah, M., Jenkinson, D. M., Vadez, V. (2011) Chickpea genotypes contrasting for seed yield under terminal drought stress in the field differ for traits related to the control of water use. Funct. Plant. Biol. 38, 270–281.
Zlatev, Z. S., Yordanov, I. T. (2004) Effects of soil drought on photosynthesis and chlorophyll fluorescence in bean plants. Bulg. J. Plant Physiol. 30, 3–18.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
About this article
Cite this article
Saglam, A., Terzi, R. & Demiralay, M. Effect of Polyethylene Glycol Induced Drought Stress on Photosynthesis in Two Chickpea Genotypes with Different Drought Tolerance. BIOLOGIA FUTURA 65, 178–188 (2014). https://doi.org/10.1556/ABiol.65.2014.2.6
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1556/ABiol.65.2014.2.6