Abstract
We investigated the presence and distribution of the D1 dopamine receptor in the CNS of Lymnaea stagnalis applying immunobloting and immunocytochemistry. We also investigated the effect of dopamine as well as the specific D1 receptor blocker, SCH23390, on the firing activity of the feeding modulator serotonergic neuron, CGC, which displayed D1 immunoreactivity. Immunoblot experiments showed one specifically labeled band with 62 kDa mw which is close to that of the mammalian D1 receptor. Neurons displaying D1-like immunoreactivity can be observed in each ganglion of the CNS but particularly in the pedal ganglia which are the center for locomotion. Dopamine regularly evokes burst activity in the serotonergic CGC at 1 mM and this effect could be antagonized by SCH23390. These observations suggest that a D1-like receptor molecule is present in the CNS of Lymnaea.
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Barron, A. B., Søvik, E., Cornish, J. L. (2010) The roles of dopamine and related compounds in reward-seeking behavior across animal phyla. Front. Behav. Neurosci. 4, 1–9.
Berry, M. S., Cottrell, G. A. (1975) Excitatory, inhibitory and bipasic synaptic potentials mediated by an identified dopamine-containing neuron. J. Physiol. Lond. 244, 589–612.
Chandhoke, V., Southall, M., Holt, R., Flinn, J. M. (2001) DA receptor subtype density as a function of age in Aplysia californica. Comp. Biochem. Physiol. 130, 461–466.
Chase, R. (2002) Behavior and its neural control in gastropod molluscs. Oxford University Press.
Chistyakova, M. V. (1990) Role of dopamine and serotonin in modulation of snail defensive behavior. Neurosci. Behav. Physiol. 20, 446–452.
Detere, P., Paupardin-Tritsch, D., Bockaert J. (1986) Serotonin and dopamine sensitive adenylate cyclase in molluscan nervous system. Biochemical and electrophysiological analysis of the pharmacological properties and the GTP-dependence. Brain Res. 387, 101–109.
Dobson, K. S., Dmetrichuk, J. M., Spencer, G. E. (2006) Different receptors mediate the electrophysiological and growth cone responses of an identified neuron to applied dopamine. Neurosci. 141, 1801–1810.
Elekes, K., Kemenes, G., Hiripi, L., Geffard, M., Benjamin, P. R. (1991) Dopamine immunoreactive neuron sin the central nervous system of the pond snail Lymnaea stagnalis. J. Comp. Neurol. 307, 214–224.
Elliott, C. J. H., Susswein, A. J. (2002) Comparative neuroethology of feeding control in molluscs. J. Exp. Biol. 205, 877–896.
Filla, A., Hiripi, L., Elekes, K. (2009) Role of aminergic (serotonin and dopamine) systems in the embryogenesis and different embryonic behaviors of the pond snail, Lymnaea stagnalis. Comp. Biochem. Physiol. 149, 73–82.
Flinn, J. M., Gochaman, P., Wanschura, P., Chandhoke, V. (2001) The effect of dopamine receptor blockade on motor behavior in Aplysia californica. Pharmacol. Biochem. Behav. 69, 425–430.
Goto, Y., Otani, S., Grace, A. A. (2007) The yin and yang of dopamine release: a new perspective. Neuropharmacol. 53, 583–587.
Grace, A. A., Floresco, S. B., Goto, Y., Lodge, D. J. (2007) Regulation of firing of dopaminergic neurons and control of goal directed behaviors. Trends Neurosci. 30, 220–227.
Green, K. A., Cottrell, G. A. (1997) Modulation of ligand gated dopamine channels in Helix neurones. Pfügers Arch.-Eur. J. Physiol. 434, 313–322.
Haque, Z., Lee, T. K. M., Inoue, T., Luk, C., Hasan, S.U., Lukowiak, K., Syed, N. I. (2006) An identified central pattern-generating neuron co-ordinates sensory-motor components of respiratory behavior in Lymnaea. Eur. J. Neurosci. 23, 94–104.
Hiroi, N., Martin, A. B., Grande, C., Alberti, I., Rivera, A., Moratalla, R. (2002) Molecular dissection of dopamine receptor signaling. J. Chemical. Neuroanat. 23, 237–242.
Hsu, S. M., Raine, L., Fanger, H. (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: comparison between ABC and unlabeled antibody (PAP) procedures. J. Histochem. Cytochem. 29, 577–580.
Kemenes, G. (1997) In vivo neuropharmacological and in vitro laser ablation techniques as tools in the analysis of neuronal circuits underlying behavior in a molluscan model system. Gen. Pharmacol. 29, 7–15.
Magoski, N. S., Bauce, L. G., Syed, N. I., Bulloch, A. G. M. (1995) Dopaminergic transmission between identified neurons from the mollusk, Lymnaea stagnalis. J. Neurophysiol. 74, 1287–1300.
Magoski, N. S., Bulloch, A. G. M. ( 1999) Dopamine activates two different receptors to produce variability in sign at identified synapse. J. Neurophysiol. 81, 1330–1340.
McKenzie, J. D., Caunce, M., Hetherington, U. S., Winlow, W. (1998) Serotonergic innervation of the foot of the pond snail Lymnaea stagnalis. J. Neurophysiol. 27, 459–470.
Missale, C., Fiorentini, C., Collo, G., Spano, P. F. (2010) The neurobiology of dopamine receptors: evolution from dual concept to heterodimer complexes. J. Receptors Signal Transduct. 30, 347–354.
Missale, C., Nash, S. R., Robinson, S. W., Jaber, M., Caron, M. G. (1998) Dopamine receptors: from structure to function. Physiol. Rev. 78, 189–125.
Monsma, F. J. Jr., Mhan, L. C., McVittie, L. D., Gerfen, C. R., Sibley, D. R. (1990) Molecular cloning and expression of a D1 DA receptor linked to adenylyl cyclase activation. Proc. Natl. Acad. Sci. USA 87, 6723–6727.
Murphy, A. D. (2001) The neuronal basis of feeding in the snail, Helisoma, with comparison to selected gastropods. Prog. Neurobiol. 63, 383–408.
Nargeot, R., Baxter, D. A., Patterson, G. W., Byrne, J. H. (1999) Dopaminergic synapses mediate neuronal changes in an analogue of operant conditioning. J. Neurophysiol. 81, 1983–1987.
Neve, K. A., Seamans, J. K., Trantham-Davidson, H. (2004) Dopamine receptor signaling. J. Receptors. Signal Transduct. 24, 165–2005.
Prudnikov, I. M., Tsyvkin, V. N., Kastrikina, T. F. (1992) GTP-dependent dopamine reception in the central nervous system tissues in the pond snail. Neurofiziologiia 24, 451–461.
Prudnikov, I. M., Osipenko, O. N., Kastrikina, T. F. Tsyvkin, V. N. (1992) Dopamine effect on ionic conduction and adenyl cyclase activity in the central nervous system of the pond snail. Neurofiziologiia 24, 437–451.
Reyes, F. D., Mozzachiodi, R., Baxter, D. A., Byrne, J. H. (2011) Reinforcement in an in vitro analog of appetitive classical conditioning of feeding behavior in Aplysia: Blockade by a dopamine antagonist. Learn. Mem. 12, 216–220.
Stoof, J. C., De Vlieger, T. A., Lodder, J. C. (1984) Opposing roles for D-1 and D-2 dopamine receptors in regulating the excitability of growth hormone-producing cells in the snail Lymnaea stagnalis. Eur. J. Pharmacol. 106, 431–435.
Straub, H., Kuhlmann, D. (1984) Identification and quantitative measurements of biogenic amines in the central nervous tissue of some gastropods. Comp. Biochem. Physiol. 78, 319–323.
Sugamori, K. S., Van Tol, H. H. M., Niznik, H. B. (1994) Invertebrate dopamine receptors. In: Niznik, H. M. (ed.) Dopamine receptors and transporters. Marcel Dekker, New York.
Walker, R. J., Brooks, H. L., Holden-Dye, L. (1996) Evolution and overview of classical transmitter molecules and their receptors. Parasitol. 113, S3–S33.
Walker, R. J., Woodruff, G. N., Glaizner, B., Sedden, C. B., Kerkut, G. A. (1968) The pharmacology of Helix dopamine receptor in specific neurons in the snail, Helix aspersa. Comp. Biochem. Physiol. 24, 455–469.
Wall, Z. V., Parker, J. G., Fadok, J. P., Darvas, M., Zweifel, L., Palmiter, R. D. (2011) A behavioral genetics approach to understanding D1 receptor involvement in phasic DA signaling. Mol. Cell. Neurosci. 46, 21–31.
Weiss, S., Drummond, G. I. (1981) Dopamine and serotonin-sensitive adenylate cyclase in the gill of Aplysia californica. Mol. Pharmacol. 20, 592–597.
Wendelaar Bonga, S. E. (1970) Ultrastructure and histochemistry of neurosecretory cells and neurohaemal areas in the pond snail Lymnaea stagnalis. Z. Zellforsch. 108, 190–224.
Zvezdochkina, N. V., Muranova, L. N., Andrianov, V. V., Arkhipova, S. S., Gainutdinov, K. H. L., Golubev, A. I., Pleshchinskii, I. N. (2006) Locomotor responses and neuron excitability in conditions of haloperidol blockade of dopamine in invertebrates and vertebrates. Neurosci. Behav. Physiol. 36, 21–27.
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Presented during the 12th ISIN Symposium on Invertebrate Neurobiology, August 31–September 4, 2011, Tihany, Hungary.
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Hernádi, L., Vehovszky, Á. & Serfőző, Z. Immunological and Pharmacological Identification of the Dopamine D1 Receptor in the CNS of the Pond Snail, Lymnaea stagnalis. BIOLOGIA FUTURA 63 (Suppl 2), 151–159 (2012). https://doi.org/10.1556/ABiol.63.2012.Suppl.2.20
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DOI: https://doi.org/10.1556/ABiol.63.2012.Suppl.2.20