Abstract
Physiological responses of Arabidopsis thaliana to the interaction of iron deficiency and nitrogen form were studied using plants grown in hydroponics. Thirty-three-day-old seedlings were submitted to four treatments for 7 days : NO3 + 5 μM Fe; NO3 + 0.1 μM Fe; NH4+5 μM Fe and NH4 + 0.1 μM Fe. Leaf growth and chlorophyll content were highest in NO3-fed, Fe sufficient plants, but were strongly diminished by Fe deficiency under nitric nutrition, and by ammoniacal nutrition independently of Fe regime. However, the leaves of NH4-fed plants presented a higher Fe content than those of Fe sufficient, NO3-fed plants. Thus, leaf chlorosis of NH4-fed in plant did not depend on Fe availability, and seemed to be due to another factor. Root acidification capacity and Fe-chelate reductase (FCR) activity were also dependent on N form. The medium was acidified under ammoniacal regime and alkalinized under nitric regime regardless of Fe level. FCR activity stimulation in response to Fe deficiency was observed only in NO3-fed plants. In addition, both N form and Fe level induced antioxidant responses in rosette leaves. Ammoniacal regime increased both peroxidase expression and anthocyanin accumulation, whereas Fe deficiency enhanced superoxide dismutase expression.
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Authors are indebted to Professor C. Grignon for stimulating discussions on this work.
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Karray-Bouraoui, N., Attia, H., Maghzaoui, M. et al. Physiological Responses of Arabidopsis thaliana to the Interaction of Iron Deficiency and Nitrogen Form. BIOLOGIA FUTURA 61, 204–213 (2010). https://doi.org/10.1556/ABiol.61.2010.2.8
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DOI: https://doi.org/10.1556/ABiol.61.2010.2.8