Abstract
Prorocentrum donghaiense caused large-scale red tides off Chinese coast in recent years. Expressed sequence tag (EST) analysis was carried out for this dinoflagellate in order to identify the genes involved in its proliferation and death. A cDNA library was constructed for P. donghaiense at late exponential growth phase, and 308 groups of EST were generated, which include 36 contigs and 272 singletons. Among 22 groups showed homologies with known genes, 2 matched significantly with caspase and proliferating cell nuclear antigen. Caspase and proliferating cell nuclear antigen are 2 key proteins involved in programmed cell death. Their identification evidenced preliminarily the induction of PCD in aging P. donghaiense. The identified included also calmodulin and protein phosphatase, two proteins involved in diverse cell processes including PCD by binding to or modifying others.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Altschul, S. F., Madden, T. L., Schaffer, A. A., Zhang, J., Zhang, Z., Miller, W., Lipman, D. J. (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25, 3389–3402.
Bachvaroff, T. R., Concepcion, G. T., Rogers, C. R., Herman, E. M., Delwiche, C. F. (2004) Dinoflagellate expressed sequence tag data indicate massive transfer of chloroplast genes to the nuclear genome. Protis. 155, 65–78.
Berges, J. A., Falkowski, P. G. (1998) Physiological stress and cell death in marine phytoplankton: induction of proteases in response to nitrogen or light limitation. Limnol. Oceanogr. 43, 129–135.
Berman-Frank, I., Bidle, K., Haramaty, L., Falkowski, P. (2004) The demise of the marine cyanobac-terium, Trichodesmium spp., via an autocatalyzed cell death pathway. Limnol. Oceanogr. 49, 997–1005.
Bidle, K. D., Falkowski, P. G. (2004) Cell death in planktonic, photosynthetic microorganisms. Nature Review. 2, 643–655.
Bowman, G. D., Goedken, E. R., Kazmirski, S. L., O’Donnell, M., Kuriyan, J. (2005) DNA poly-merase clamp loaders and DNA recognization. FEBS Lett. 579, 863–867.
Brussaard, C. P. D., Noordeloos, A. A. M., Riegman R. (1997) Autolysis kinetics of the marine diatom Ditylurn brightwellii (Bacillariophyceae) under nitrogen and phosphorus limitation and starvation. J. Phycol. 33, 980–987.
Crépineau, F., Roscoe, T., Kaas, R., Kloareg, B., Boyen, C. (2000) Characterization of complementary DNAs from the expressed sequence tag analysis of life cycle stages of Laminaria digitata (Phaeophyceae). Plant Molecular Biolog. 43, 503–513.
Danial, N. N., Korsmeyer, S. J. (2004) Cell death: critical control points. Cel. 116, 205–219.
Field, C. B., Behrenfeld, M. J., Randerson, J. T., Falkowski, P. (1998) Primary production of the biosphere: Integrating terrestrial and oceanic components. Scienc. 281, 237–240.
Hackett, J. D., Yoon, H. S., Soares, M. B., Bonaldo, M. F., Casavant, T. L., Scheetz, T. E., Nosenko, T., Bhattacharya, D. (2004) Migration of the plastid genome to the nucleus in a peridinin dinoflagel-late. Current Biolog. 14, 213–218.
Huang, X., Madan, A. (1999) CAP3: a DNA sequence assembly program. Genome Res. 9, 868–877.
Lu, D. D., Qi, Y. Z., Jeanette, G., Zou, J. Z., Gao, Y. H. (2003) Re-description of Prorocentrum dong-haiense Lu and comparison with relevant Prorocentrum species. Chinese Journal of Applied Ecolog. 14, 1060–1064.
Montsant, A., Jabbari, K., Maheswari, U., Bowler, C. (2005) Comparative genomics of the pinnate diatom Phaeodactylum tricornutum. Plant Physiolog. 137, 500–513.
Ning, S. B., Guo, H., L., Wang, L., Song, Y. C. (2002) Salt stress induces programmed cell death in prokaryotic organism Anabaena. J. Appl. Microbiol. 93, 15–28.
Paunesku, T., Mittal, S., Protic, M., Oryhon, J., Korolev, S. V., Joachimiak, A., Woloschak, G. E. (2001) Proliferating cell nuclear antigen (PCNA): ringmaster of the genome. International Journal of Radiation Biolog. 77, 1007–1021.
Qi, Y. Z., Wang, Y. (2003) What the Prorocentrum species should be? A review on identification of a Prorocentrum species from the East China Sea. Chinese Journal of Applied Ecology 14, 1188–1190.
Scala, S., Carels, N., Falciatore, A., Chiusano, M. L., Bowler, C. (2002) Genome properties of the diatom Phaeodactylum tricornutum. Plant Physiol. 129, 993–1002.
Schroeder, D. C., Oke, J., Malin, G., Wilson, W. H. (2002) Coccolithovirus (Phycodnaviridae): characterization of a new large dsDNA algal virus that infects Emiliania huxleyi. Arch. Virol. 147, 1685–1698.
Segovia, M., Haramaty, L., Berges, J. A., Falkowski, P. G. (2003) Cell death in the unicellular chloro-phyte Dunaliella tertiolecta. A hypothesis on the evolution of apoptosis in higher plants and meta-zoans. Plant Physiolog. 132, 99–105.
Shrager, J., Hauser, C., Chang, C. W., Harris, E. H., Davies, J., McDermott, J., Tamse, R., Zhang, Z., Grossman, A. R. (2003) Chlamydomonas reinhardtii genome project: A guide to the generation and use of the cDNA information. Plant Physiol. 131, 401–408.
Tanikawa, N., Akimoto, H., Ogoh, K., Chun, W., Ohmiya, Y. (2004) Expressed sequence tag analysis of the dinoflagellate Lingulodinium polyedrum during dark phase. Photochem. Photobiol. 80, 31–35.
Vardi, A., Berman-Frank, I., Rozenberg, T., Hadas, O., Kaplan, A., Levine, A. (1999) Programmed cell death of the dinoflagellate Perdinium gatunense is mediated by CO2 limitation and oxidative stress. Curr. Biol. 9, 1061–1064.
Wahlund, T. M., Hadaegh, A. R., Clark, R., Nguyen, B., Fanelli, M., Read, B. A. (2004) Analysis of expressed sequence tags from calcifying cells of marine coccolithophorid (Emiliania huxleyi). Marine Biotechnolog. 6, 278–290.
Wang, J. H., Huang, X. Q. (2003) Ecological characteristics of Prorocentrum dentatum and the cause of harmful algal bloom formation in China Sea. Chinese Journal of Applied Ecolog. 14, 1065–1069.
Zhang, B. Y., Wang, G. C., Qi, Y. Z., Zou, J. Z., Zeng, C. K. (2005) Identification of two species of dinoflagellate using fluorescence in situ hybridization. High Technology Letter. 15, 101–105.
Zhang, B. Y., Wang, G. C., Zhang, Y., Han, X. T., Lu, S. J., Qi, Y. Z., Zou, J. Z., Zeng, C. K. (2004) Cloning and sequence analysis of 5.8s rDNA and ITS region from Prorocentrum donghaiense and P. micans APBM. Oceanologia et Limnologia Sinic. 35, 264–272.
Acknowledgements
This work work was supported by grants from The Key Laboratory of Mariculture of Education Ministry of China affiliated to Ocean University of China (Grant No. 200405), National Natural Science Foundation of China (Grant No. 30471318) and The Ministry of Science and Technology of China (Grant No. 2005CCA02400) to G. Yang, and a grant from The Ministry of Science and Technology of China (Grant No. 2001CB409700) to M. Zhu.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
About this article
Cite this article
Zhang, X., Yang, G., Liu, Y. et al. Induction of Programmed Cell Death in Aging Prorocentrum donghaiense Cells as was Evidenced Preliminarily by the Identification of Associated Transcripts. BIOLOGIA FUTURA 57, 473–483 (2006). https://doi.org/10.1556/ABiol.57.2006.4.9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1556/ABiol.57.2006.4.9