Abstract
Using confocal microscopic analysis, FITC-labelled anti-α-tubulin antibody and the fluorescent taxol derivative Flutax-1 in fixed and living Tetrahymena pyriformis GL, longitudinal microtubules, oral and somatic cilia, deep fibers, and contractile vacuole pores were equally labeled. While the antibody stained transversal microtubules, these were not labeled by Flutax-1. At the same time, oral cilia were more intensely stained by Flutax-1, than by the antibody. There were no differences in the staining of fixed preparations and living cells. The observations suggest (i) the difference between the MAPs of longitudinal and transversal microtubules which allow or inhibit the binding of the indicator molecules, and (ii) the different functions of these two types of microtubules.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Andreu, J. M., Diaz, J. F., Gil, R., de Pereda, J. M., Garcia de Lacoba, M., Peyrot, V., Briand, C., Towns-Andrews, E., Bordas J. (1994) Solution structure of Taxotere-induced microtubules to 3-nm resolution. The change in protofilament number is linked to the binding of the taxol side chain. J. Biol. Chem. 269, 31785–31792.
Arregui, L., Munoz-Fontel, C., Serrano, S., Barasoain, I., Guinea, A. (2002) Direct visualization of the microtubular cytoskeleton of ciliated protozoa with a fluorescent taxoid. J. Eukaryot. Microbiol. 49, 312–318.
Aufderheide, K. J., Frankel, J., Williams, N. E., (1980) Formation and positioning of surface-related structures in Protozoa. Microbiol. Rev. 44, 252–302.
Diaz, J. F., Barasoain, I., Andreu, J. M., (2003) Fast kinetics of taxol binding to microtubules. Effects of solution variables and microtubule-associated proteins. J. Biol. Chem. 278, 8407–8419.
Evangelio, J. A., Abal, M., Barasoain, I., Souto, A. A., Lillo, M. P., Acuna, A. U., Amat-Guerri, F., Andreu, J. M., (1998) Fluorescent taxoids as probes of the microtubule cytoskeleton. Cell Motility Cytoskeleton 39, 73–90.
Gaertig, J. (2000) Molecular mechanism of microtubular organelle assembly in Tetrahymena. J. Eukaryot. Microbiol. 47, 185–190.
Kovács, P., Csaba, G. (2005) Effect of drugs affecting microtubular assembly on microtubules, phospholipid synthesis and physiological indices (signalling, growth, motility and phagocytosis) in Tetrahymena pyriformis. Cell Biochem. Funct. 23, 1–11.
Kovács, P., Csaba, G., Czaker, R. (2005) Effects of taxol treatment on the microtubular system and mitochondria of Tetrahymena. Acta Protozool. (in press).
Lynn, D. H. (1988) Cytoterminology of cortical components of ciliates: somatic and oral kinetids. BioSystems 21, 299–307.
Margolis, R. L., Wilson, L. (1978) Opposite end assembly and disassembly of microtubules at steady state in vitro. Cell 13, 1–8.
Nogales, E., Whitakker, M., Milligan, R. A., Downing, K. H., (1999) High-resolution model of the microtubule. Cell 96, 79–88.
Pazour, G. J., Agrin, N., Leszik, J., Witman, G. B., (2005) Proteomic analysis of a eukaryotic cilium. J. Cell Biol. 170, 103–113.
Acknowledgements
This work was supported by the Hungarian Scientific Research Fund (OTKA-T-037303), Hungary.
Author information
Authors and Affiliations
Rights and permissions
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
About this article
Cite this article
Kovács, P., Csaba, G. Comparison of the Binding of Anti-Tubulin Antibody and the Fluorescent Taxol Derivative Flutax-1 to the Microtubular System of Tetrahymena. BIOLOGIA FUTURA 57, 323–329 (2006). https://doi.org/10.1556/ABiol.57.2006.3.6
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1556/ABiol.57.2006.3.6