Abstract
Membrane lipids play an important role in the function of blood platelets but the mechanisms by which the lipid composition of the platelet membrane is adjusted remain unclear. It has been shown that stress and poly-unsaturated fatty acids modified the lipid composition of blood plasma and platelet lipids, but very little is known about the effect of stress and fatty acids on membrane platelet lipid composition. The purpose of the present investigation was to study the influence of the essential fatty acids: linoleic, linolenic and arachidonic acids on the composition of the platelet membrane lipids of rats assigned to heat and restraint stress. It was shown that injections of polyunsaturated fatty acids decrease or suppress the stress-induced increase in platelet aggregation, suppress the stress-induced modification of the composition of the platelet membrane lipids and modify the fatty acid composition of the platelet membrane phospholipids.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- PE:
-
phosphatidyl-ethanolamine
- PC:
-
phosphatidyl-choline
- PS:
-
phosphatidyl-serine
- PI:
-
phosphatidyl-inositol
- Sph:
-
sphingomyelin
- AP:
-
phosphatidic acid
- Cer:
-
cerebrosides
- Sulf:
-
sulfatides
- GG:
-
gangliosides
References
Bacon, S. L., Watkins, L. L., Babyak, M., Sherwood, A., Hayano, J., Hinderliter, A. L., Waugh, R., Blumenthal, J. A. (2004) Effects of daily stress on autonomic cardiac control in patients with coronary artery disease. Am. J. Cardiol. 93, 1292–1294.
van den Bosch, H. (1982) On the mechanisms of biosynthesis of leukotrienes and related compounds. In: Hawthorne, J. N., Ansell, G. B. (eds) Phospholipids. Elsevier, Amsterdam, pp. 313–358.
Braun, P. E., Radin, N. S. (1969) Interactions of lipids with a membrane structural protein from myelin. Biochemistry 8, 4310–4318.
Carruthers, A., Melchior, D. L. (1988) Role of the bilayer lipids in governing membrane transport processes. In: Aloia, R. C., Curtain, C. C., Gordon, L. M. (eds) Lipid Domains and the Relationship to Membrane Function. Alan R. Liss Inc. New York, pp. 201–225.
Chap, H., Zwaal, R. F. A., van Deenern, L. L. M. (1977) Action of highly purified phospholipases on blood platelets: evidence for an asymetrical distribution of phospholipids in the surface membrane. Biochim. Biophys. Acta 467, 146–164.
Chiang, T. M., Khang, A. H., Fain, J. N. (1991) Stimulation of PLA2 activity in human platelets by trypsin and collagen. Arch. Biochem. Biophys. 284, 47–52.
Coulon L., Calzada, C., Moulin, P., Vericel, E., Lagarde, M. (2003) Activation of p38 mitogen-activated protein kinase/cytosolic phospholipase A2 cascade in hydroperoxide-stressed platelets. Free Radic. Biol. Med. 35, 616–625.
Davis, P. B., Silki, C. (1987) Ageing and α2 adrenergic system of the platelet. Clinical Science 73, 507–513.
Dawson, R. M. C. (1960) A hydrolytic procedure for the identification and estimation of individual phospholipids in biological samples. Biochem. J. 75, 45–53.
Dawson, A. A., Ogston, D. (1969) Exercise-induced thrombocytosis. Acta Haematologica 42, 241–246.
Demaison, L., Moreau, D. (2002) Dietary n-3 polyunsaturated fatty acids and coronary heart diseaserelated mortality: a possible mechanism of action. Cell. Mol. Life Sci. 59, 463–477.
Dewey, M., Hamm, B. (2004) Cost-effectiveness comparison of the most common diagnostic modalities in coronary artery disease. Dtsch. Med. Wochenschr. 129, 1415–1419.
Ersoz, G., Zergeroglu, A. M., Ficicilar, H., Ozcan, H., Oztekin, P., Aytac, S., Yavuzer, S. (2002) Effect of submaximal and incremental upper extremity exercise on platelet function and the role of blood shear stress. Thromb. Res. 108, 297–301.
Friedman, M., Rosenman, R. H. (1959) Association overt behavior pattern with blood and cardiovascular findings. J.A.M.A. 169, 1286–1292.
Gozlan-Devilliere, N., Baumann, N., Bourre, J. M. (1978) Incorporation of stearic acid into brain lipids in the developing brain: blood-brain barrier relationships during development. Dev. Neurosci. 1, 153–158.
Gjerlff-Schmidt, K., Waever-Rasmussen, J. (1984) Exercise-induced changes in the in vivo distribution of 111In-labelled platelets. Scand. J. Haematol. 32, 159–166.
Gross, P. L., Rand, M. L., Barrow, D. V., Packham, M. A. (1991) Platelet hypersensitivity in cholesterol-fed rabbits: enhancement of thromboxane A2-dependent and thrombin-induced, thromboxane A2-independent platelet responses. Atherosclerosis 88, 77–86.
Haft, J. J. (1979) Role of blood platelets in coronary heart disease. Am. J. Cardiology 43, 1197–1206.
Hammerschmidt, D. E. (1982) Platelets and the environment. J.A.M.A. 247, 345–350.
Heemskerk, J. W. M., Feijge, M. A. H., Kalafusz, R., Hornstra, G. (1989) Influence of dietary fatty acids on membrane fluidity and activation of rat platelets. Biochim. Biophys. Acta 1004, 252–260.
Heemskerk, J. W. M., Feijge, M. A. H., Reitman, E., Hornstra, G. (1991) Rat platelets are deficient in internal Ca++ release and require influx of intracellular Ca++ for activation. FEBS Lett. 284, 223–226.
Heemskerk, J. W., Feijge, M. A., Simonis, M. A., Hornstra, G. (1995) Effects of dietary fatty acids on signal transduction and membrane cholesterol content in rat platelets. Biochim. Biophys. Acta 1255, 87–97.
Huang, Y. S., Mills, D. E., Ward, R. P., Simmons, V. A., Horrobin, D. F. (1990) Stress modulates cholesterol- induced changes in plasma and liver fatty acid deposition in rats fed n-6 fatty acid rich oils. Proc. soc. Exp. Biol. Med. 135, 136–141.
Hunter, K. A., Crosbie, L. C., Weir, A., Miller, G. J., Dutta- Roy, A. K. (2000) A residential study comparing the effects of diets rich in stearic acid, oleic acid, and linoleic acid on fasting blood lipids, hemostatic variables and platelets in young healthy men. J. Nutr. Biochem. 11, 408–416.
Jern, C., Wadenvik, H., Mark, H., Haligren, J., Jern, S. (1989) Haematological changes during acute mental stress. Br. J. Haematol. 71, 153–156.
den Kamp, D. P., Jos, A. F. (1979) Lipid asymmetry in membranes. Ann. Rev. Biochem. 48, 47–52.
Larsson, P. T., Hjemdahl, P., Olsson, G., Egberg, N., Hornstra, G. (1989) Altered platelet function during mental stress and adrenaline infusion in humans; evidence for an increased aggregability in vivo as measured by filtragometry. Clinical Sciences 76, 369–376.
Levine, S. P., Towell, B. L., Suarez, A. M., Knieriem, L. K., Harris, M. M., George, J. N. (1985) Platelet addiction and secretion associated with emotional stress. Circulation 71, 1129–1134.
Liesse, M., van Imscitout, K., Mertens, C., van der Abbeele, K. G., Lauwers, P. (1980) Evolution temporelle du fibrinogène et du nombre de plaquettes en réponse au stress. Acta Psychiatrica Belgica 80, 34–44.
Mahadevappa, V. G., Holub, B. J. (1982) The molecular species composition of individual diacylphospholipids in human platelets. Biochem. Biophys. Acta 713, 73–79.
Marcus, A. J. (1978) The role of lipids in platelet function with particular reference to arachidonic acid pathway. J. Lipid Res. 19, 793–826.
Mest, H. J., Zehl, V., Sziegoleit, W., Taube, Ch., Ferster, W. (1982) Influence of mental stress in plasma level of prostaglandin thromboxane B2 and circulating platelet aggregates in man. Prostaglandins, Leukotrienes and Medicine 8, 553–563.
Miettinen, I. T., Takki- Luukainen, I. T. (1959) Use of butyl acetate in determination of sialic acid. Acta Chem. Scand. 13, 856–858.
Morin, R. J. (1980) The role of phospholipids in platelet function. Am. Clin. Lab. Sci. 10, 463–468.
Morrison, W. M., Smith, L. R. (1964) Preparation of fatty acid methyl esters and dimethyl acetals from lipids with boron-fluoride methanol. J. Lipid Res. 5, 600–608.
Neskovic, M. L., Sarlieve, J. L., Nussbaum, D., Mandel, P. (1972) Quantitative thin-layer chromatography of glycolipids in animal tissues. Clin. Chim. Acta 38, 147–153.
Nouvelot, A., Bourre, J. M., Sezille, G., Dewailly, P., Jaillard, J. (1983) Changes in the fatty acid patterns of brain phospholipids during development of rats fed peanuts or rapeseed oil taking into account differences between milk and maternal food. Ann. Nutr. Metab. 27, 173–181.
Picke, L. A., Mahadevappa, V. G. (1990) Effect of dietary lipids in collagen-induced and ADPinduced platelet aggregation and thromboxane A2 synthesis in the rat. J. Nutritional Biochemistry 1–4, 206–212.
Pollet, S., Ermidou, S., Le Saux, F., Monge, M., Baumann, N. (1978) Microanalysis of brain lipids: multiple two-dimensional thin-layer chromatography. J. Lipid. Res. 19, 916–921.
Sarajas, M. S. S., Kontinen, A., Frick, M. H. (1961) Thrombocytosis evoked by exercise. Nature 192, 721–722.
Searcy, R. L., Bergquist, J. L. (1960) A new color reaction for the quantification of serum cholesterol. Clin. Chim. Acta 5, 192–199.
Schick, P. K., Korica, K. B., Chacko, C. K. (1976) Location of phosphatidyl-ethanolamine and Phosphatidyl-serine in the human platelet plasma membrane. J. Clin. Invest. 57, 1221–1226.
Shinitzky, M. (1984) Membrane fluidity in malignancy. In: Shinitzky, M. (ed.) Physiology of Membrane Fluidity. Vol. 1. CRC. Press, Boca Raton, pp. 1–51.
Sprecher, H. (1987) Oxygenated metabolism of minor fatty acids of the (n-6) and (n-3) families. In: Lagarde, M. (ed.) Biology of the Icosanoids. Vol. 152. Colloque INSERM, pp. 77–86.
Srivastava, K., Dash, D. (2001) Altered membrane fluidity and signal transduction in the platelets from patients of thrombotic stroke. Mol. Cell Biochem. 224, 143–149.
Stuart, M. J., Gerrard, J. M., White, J. G. (1980) Effect of cholesterol on production of thromboxane b2 by platelets in vitro. New Engl. J. Med. 302, 6–10, 62–67.
Stubbs, C. D., Smith, A. D. (1984) The modification of mammalian membrane poly-unsaturated fatty acid composition in relation to membrane fluidity and function. Biochim. Biophys. Acta 779, 89–137.
Svennerholm, L. (1956) The quantitative estimation of cerebrosides in nerve tissue. J. Neurochem. 1, 42–53.
Tesserommatis, C., Tsopanakis, C., Symeonoglou, G., Loukissa, M., Kouvarou, E., Varonos, D. D. (1996) How harmless is FFA enhancement? Eur. J. Drug. Metab. Pharmacokinet. 21, 213–215.
Tozzi-Ciancarelli, M. G., Penco, M., Di Massimo, C. (2002) Influence of acute exercise on human platelet responsiveness: possible involvement of exercise-induced oxidative stress. Eur. J. Appl. Physiol. 86, 266–272.
Vilen, J., Freden, K., Kutti, J. (1980) Presence of a non splenic platelet pool in man. Scand. J. Haematol. 24, 137–141.
Vlasic, N., Medow, M. S., Medow, S. M., Schwartz, S. M., Pritchard, Jr., K. A., Stemerman, M. B. (1993) Lipid fluidity modulates platelet aggregation and agglutination in vitro. Life Sci. 53, 1053–1069.
Weber, P. C. (1987) The dietary modification of the arachidonic cascade. In: Lagarde, M. (ed.) Biology of the Icosanoids. Vol. 152. Colloque INSERM, pp. 119–126.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
About this article
Cite this article
Ermidou-Pollet, S., Nounopoulos, H., Sdougas, N. et al. Influence of Subcutaneous Injection of Essential Fatty Acids on the Stress-Induced Modifications of Rat Platelet Aggregation and Membrane Lipid Composition. BIOLOGIA FUTURA 56, 247–259 (2005). https://doi.org/10.1556/ABiol.56.2005.3-4.8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1556/ABiol.56.2005.3-4.8