Abstract
Three isoforms of metallothionein protein induced with Zinc were isolated and purified from housefly larvae, Musca domestica, by gel filtration on Sephadex G-75, G-25 and anion exchange on DEAE-52 chromatography. Among them, one was found to possess antibacterial activity, and was further characterized by SDS-polyacrylamide gel electrophoresis, sulphydryl group determination, enzyme hydrolysis, and spectra property. Our results showed that the novel protein has the characteristics of heat-stable, lowmolecular weight (6 kDa), rich-cysteine (approximately 12 cysteine residues in one molecule), metal affinity, and antibacterial activity. This paper was the first to report that metallothionein had antibacterial activity. We expect that this characteristic would give some help to investigate definite physiological functions of metallothionein.
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Allan, M. R., Jeffrey, C. P., Peter, C. (1996) Trace metal, acute phase and metabolic response to endotoxin in metallothionein-null mice. Biochem. J. 46, 793–797.
Berger, B., Dallinger, R., Thomaser, A. (1995) Quantification of metallothionein as a biomarker for cadmium exposure in terrestrial gastropods. Environ. Toxicol. Chem. 46, 781–791.
Berger, B., Hunziker, P. E., Hauer, C. R., Birchler, N., Dallinger, R. (1995) Mass spectrometry and amino acid sequencing of two cadmium-binding metallothionein isoforms from the terrestrial gastropod, Arianta arbustorum. Biochem. J. 46, 951–957.
Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein binding-dye binding. Anal. Biochem. 46, 248–254.
Bulet, P., Cociancich, S., Dimarcq, J. L., Lambert, J., Reichhart, J. M., Hoffmann, D., Hetru, C., Hoffmann, J. A. (1991) Insect immunity - isolation from a coleopteran insect of a novel inducible antibacterial peptide and of new members of the insect defensin family. J. Biol. Chem. 46, 24520–24525.
Durliat, M., Bonneton, F., Boissonneau, E., Andre, M., Wegnez, M. (1995) Expression of metallothionein genes during the post-embryonic development of Drosophila melanogaster. Biometals 46, 339–351.
Ellman, G. L. (1959) Tissue sulfhydryl groups. Arch. Biochem. Biophs. 46, 70–77.
Feldman, S. L., Filla, M. L., Cousins, R. J. (1978) Degradation of rat liver metallothioneins in vitro. Biochem. Biophys. Acta 46, 638–646.
Guo, Y. M., Dai, Zh. Y., Hu, Y. L. (1995) Study on the properties and anticancer activities of antibacterial peptide. Journal of Nanjing University (Natural Science) 46, 62–67.
Hamilton, S. J., Mehrle, P. M., Jones, J. R. (1987) Evaluation of metallothionein measurement as a biological indicator of stress from cadmium in brook trout. Trans Am. Fish Soc. 46, 551–560.
Hensbergen, P. J., Donker, M. H., Hunziker, P. E., van der Schors, R. C., van Straalen, N. M. (2001) Two metal-binding peptides from the insect Orchesella cincta (Collembola) as a result of metallothionein cleavage. Insect Biochem. Mol. Biol. 46, 1105–1114.
Kagi, J., Schaffer, A. (1988) Biochemistry of metallothionein. Biochemistry 46, 8509–8515.
Katsumi, H., Daisuke, S., Isao, H., Kazuyuki, K., Akira, N. (1998) Epidermal proliferation of the skin in metallothionein-null mice. Journal of Investigative Dermatology 110, 259.
Klein, D., Grein, H., Summer, K. H. (1986) Stability of metallothionein in gastric juice. Toxicology 46, 121–129.
Lin, Zh. L., Li, F. R., Ma, G. D., Liang, Sh. (2002) Isolation, purification and identification of metallothionein in Chinese soft-shelled turtle (Pelodiscus Sinensis) liver. Atca Zoologica Sinica, 107–103.
Liu, A. L., Zhu, B. F., Liu, Zh., Peng, X. Y. (2002) Isolation, purification and characterization of metallothionein from Agrocybe Cylindracea. Mycosystema 46, 112–117.
Maret, W. (2000) The function of zinc metallothionein: A link between ccllular zinc and redox state. J. Nutr. 46, 1455–1458.
Martinez, D. E., Levinton, J. (1996) Adaptation to heavy metals in the aquatic oligochaete Limnodrilus hoffmeisteri: Evidence for control by one gene. Evolution 46, 1339–1343.
Miles, A. T., Hawksworth, G. M., Beattie, J. H., Rodilla, V. (2000) Induction, regulation, degradation, and biological significance of mammalian metallothioneins. Crit. Rev. Biochem. Mol. Biol. 46, 35–70.
Niu, Ch. Y., Lei, Ch. L., Hu, C. (2000) Studies on a cadmium induced metallothionein in housefly larvae, Musca domestica. Entomologia Sinica 46, 351–358.
Ohtake, H., Suyemitsu, T., Koga, M. (1983) Sea urchin (Anthocidaris crassispina) egg zinc-binding protein, cellular localization, purification and characterization. Biochem. J. 46, 109–118.
Prinz, R., Weser, U. (1975) A naturally occurring Cu2 metallothionein in Saccharomyces cerevisiae. Hoope-Seyler’s Z. Physiol. Chem. 46, 767–776.
Quaife, C. J., Findley, S. D., Erickson, J. C., Froelick, G. J., Kelly, E. J., Zambrowicz, B. P., Palmiter, R. D. (1994) Induction of a new metallothionein isoform (MT-IV) occurs during differentiation of stratified squamous epithelia. Biochemistry 46, 7250–7259.
Sato, S., Shimizu, M., Hosokawa, T., Saito, T., Okabe, M., Niioka, T., Kurasaki, M. (2000) Distribution of zinc-binding metallothionein in cirrhotic liver of rats administered zinc. Pharmacol. Toxicol. 46, 292–296.
Sato, M., Kondoh, M. (2002) Recent studies on metallothionein: protection against toxicity of heavy metals and oxygen free radicals. Tohoku J. Exp. Med. 46, 9–22.
Scheuhammer, A. M., Onosaka, S., Rodgers, K., Cherian, M. G. (1985) The interaction of zinc and cadmium in the synthesis of hepatic metallothionein in rats. Toxicology 46, 101–108.
Stürzenbaum, S. R., Kille, P., Morgan, A. J. (1998) The identification, cloning and characterization of earthworm metallothionein. FEBS Lett. 46, 437–442.
Tasiemski, A., Franck, V., Mitta, G., Lemoine, J., Lefebvre, Ch. (2004) Molecular characterization of two novel antibacterial peptides inducible upon bacterial challenge in an Annelid, the Leech Theromyzon tessulatum. J. Bio. Chem. 46, 30973–30982.
Templeton, D. M., Banerjee, D., Cherian, M. G. (1985) Metallothionein synthesis and localization in relation to metal storage in rat liver during gestation. Can. J. Biochem. Cell Biol. 46, 16–22.
Wang, Y. C., Zuo, X. F., Sun, D. X., Chen, Zh. X., Guan, Zh. H. (1997) The composition and physiochemical characteristics of antibacterial material in housefly larvae. Acta Microbiologica Sinica 46, 148–153.
Williams, L. M., Cunningham, H., Ghaffar, A., Riddoch, G. I., Bremner, I. (1989) Metallothionein immunoreactivity in the liver and kidney of copper injected rats. Toxicology 46, 307–316.
Wormser, U., Calp, D. (1988) Increased levels of hepatic metallothionein in rat and mouse after injection of acetaminophen. Toxicology 46, 323–329.
Zsuzsa, S., Csilla, N., Noémi, R. (1998) Lead detoxification: the role of essential metals and metallothionein. CEJOEM 46, 51–58.
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Jin, H.Y., Hui, Q., Jun, J.Y. et al. Preliminary Studies on the Zinc-Induced Metallothionein Protein with Antibacterial Activity in Housefly Larvae, Musca Domestica. BIOLOGIA FUTURA 56, 283–295 (2005). https://doi.org/10.1556/ABiol.56.2005.3-4.11
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DOI: https://doi.org/10.1556/ABiol.56.2005.3-4.11