Abstract
In this study, chromosome number and ploidy levels of Ipheion uniflorum cv. “Wisley Blue” (spring starflower) were determined. In meristematic root tip cells, chromosome number was found as 2n=12 and 4n=24. The ratios of diploid and tetraploid cells were found as 80.74% and 19.26%, respectively. In differentiated root tissues and mature leaf tissues ploidy levels were analysed by flow cytometry and polysomaty were found in both organs. In differentiated root tissues, ploidy levels were found as 2C, 4C, 8C and 16C DNA. In root tissues percentages of 2C, 4C, 8C and 16C nuclear DNA content were observed as 57.2%, 33.1%, 2.47% and 7.23%, respectively. In mature leaf tissues, ploidy levels were determined 2C, 4C, 8C and 16C DNA. In this tissue the frequency of 4C DNA was found very higher (74.3%) and 2C DNA content was determined as 19.2%. In mature leaf tissue, 8C and 16C nuclear DNA contents were observed as 2.72% and 3.78%, respectively. When nuclear DNA contents in leaves and roots were compared, an apparent difference in 2C and 4C DNA contents was found.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Dane, F., Dalgic, G., Meric, C. (2002) Karyological studies on Ipheion uniflorum (R. C. Graham) Rafin (Liliaceae). 16th National Biology Congress, Abstracts, p. 149.
Darlington, C. D., Janaki Ammal, E. K. (1945) Chromosome Atlas of Cultivated Plants. George Allen & Unwin Ltd., London.
De Rocher, E. J., Harkins, K. R., Galbraith, D. W., Bohnert, H. J. (1990) Developmentally regulated systemic endopolyploidy in succulents with small genomes. Science 250, 99–101.
De Schepper, S., Leus, L., Mertens, M., Van Bockstaele, E., De Loose, M., Debergh, P., Heursel, J. (2001) Flow cytometric analysis of ploidy in Rhododendron (subgenus Tsutsusi). HortScience 36, 125–127.
Galbraith, D. W., Harkins, K. R., Maddox, J. M., Ayres, N. M., Sharma, D. P., Firoozabady, E. (1983) Rapid flow cytometric analysis of the cell cycle in intact plant tissues. Science 220, 1049–1051.
Gilissen, L. J. W., Van Staveren, M. J., Creemers-Molenaar, J., Verhoeven, H. A. (1993) Development of polysomaty in seedlings and plants Cucumis sativus L. Plant Sci. 91, 171–179.
Kudo, N., Kimura, Y. (2001) Flow cytometric evidence for endopolyploidy in seedlings of some Brassica species. Theor. Appl. Genet. 102, 104–110.
Melaragno, J. E., Mehrotra, B., Coleman, A. W. (1993) Relationship between endopolyploidy and cell size in epidermal tissues of Arabidopsis. Plant Cell 5, 1661–1668.
Mishiba, K., Mii, M. (2000) Polysomaty analysis in diploid and tetraploid Portulaca grandiflora. Plant Sci. 156, 213–219.
Phillips, R., Rix, M. (1989) Ipheion uniflorum (R. C. Graham) Rafin. In: Mathew, B. (ed.) Bulbs. Pan Books Ltd., London, p. 127.
Purvis, M. J., Collier, D. C., Walls, D. (1966) Laboratory Techniques in Botany. Second Edition. Butterworth Inc., Washington.
Sesek, P., Sustar-Vozlic, J., Bohanec, B. (2000) Determination of aneuploids in hop (Humulus lupulus L.) using flow cytometry. Eur. J. Physiol. 439, 16–18.
Smulders, M. J. M., Rus-Kortekaas, W., Gilissen, L. J. W. (1994) Development of polysomaty during differentiation in diploid and tetraploid tomato (Lycopersicon esculentum) plants. Plant Sci. 97, 53–60.
Tuna, M., Vogel, K. P., Arumuganathan, K., Gill, K. S. (2001) DNA content and ploidy determination of bromegrass germplasm accessions by flow cytometry. Crop Sci. 41, 1629–1634.
Yanpaisan, W., King, N. J. C., Doran, P. M. (1999) Flow cytometry of plant cells with applications in large-scale bioprocessing. Biotechnol. Adv. 17, 3–27.
Zonneveld, B. J. M., Van Iren, F. (2000) Flow cytometric analysis of DNA content in Hosta reveals ploidy chimeras. Euphytica 111, 105–110.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
About this article
Cite this article
Meric, C., Dane, F. Determination of Ploidy Levels in Ipheion uniflorum (R. C. Graham) Rafin (Liliaceae). BIOLOGIA FUTURA 56, 129–136 (2005). https://doi.org/10.1556/ABiol.56.2005.1-2.13
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1556/ABiol.56.2005.1-2.13