Abstract
The total of 98 strains of moulds were isolated from soils collected in arctic tundra (Spitzbergen). Among these strains Penicillium cyclopium 1, the most effective for production of catalase, was selected by the method of test-tube microculture. The time course of growth and catalase production by this strain showed the intracellular activity of this enzyme to be about 3-fold higher than its extracellular level. Some properties of crude catalase preparation, isolated from postculture liquids by lyophilization, were also examined. Catalase activity showed its maximum at 15 °C, indicating adaptation of the enzyme to lower temperatures of the arctic environment.
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Akertek, D., Tarhan, L. (1995) Characteristic of immobilized catalases and their application in pasterization of milk with H2O2. Appl. Biochem. Biotechnol. 50, 291–303.
Bradner, J. R., Gillings, M., Nevalainen, K. M. H. (1999) Qualitative assessment of hydrolytic activities in antarctic microfungi grown at different temperatures on solid media. World J. Microbiol. Biotechnol. 15, 143–145.
Caridis, K. A., Christakopoulos, P., Macris, B. J. (1991) Simultaneous production of glucose oxidase and catalase by Alternaria alternata. Appl. Microbiol. Biotechnol. 34, 794–797.
Domsch, K. H., Gamsch, W., Anderson, T. H. (1980) Compendium of Soil Fungi. Vol. 1, Academic Press, London.
Feller, G., Thiry, M., Arpigny, J. L., Mergeay, M., Gerday, C. (1989) Lipases from psychrotrophic antarctic bacteria. FEMS Microbiol. Lett. 66, 239–244.
Fiedurek, J., Gromada, A. (1997) Selection of biochemical mutants of Aspergillus niger with enhanced catalase production. Appl. Microbiol. Biotechnol. 47, 313–316.
Fiedurek, J., Gromada, A. (2000) Production of catalase and glucose oxidase by Aspergillus niger using unconventional oxygenation of culture. J. Appl. Microbiol. 89, 85–89.
Fraaije, M. W., Roubroeks, H. P., Hagen, W. R., Vanberkel, W. J. H. (1996) Purification and characterization of an intracellular catalase-peroxidase from Penicillium simplicissimum. Europ. J. Biochem. 235, 192–198.
Frost, M. G., Moss, D. A. (1987) Production of enzymes by fermentations. In: Rehm, H. J., Reed, G. (eds), Biotechnology VCH Verlagsgesellschaft, Weinheim, Vol. 7a, pp. 108–110.
Horikoshi, K. (1995) Discovering novel bacteria, with an eye to biotechnological applications. Curr. Opin. Biotechnol. 6, 292–297.
Margesin, R., Schinner, F. (1994) Properties of cold-adapted microorganisms and their potential role in biotechnology. J. Biotechnol. 33, 1–14.
Nishikawa, Y., Kawata, Y., Nagai, J. (1993) Effect of triton on catalase production by Aspergillus terreus IFO6123. J. Ferment. Bioeng. 76, 235–236.
Norton, S., Vuillemard, X. (1994) Food bioconversion and metabolite production using immobilized cell technology. Crit. Rev. Microbiol. 14, 193–224.
Onions, A. H. S., Brady, B. L. (1987) Taxonomy of Penicillium and Acremonium. In: Atkinson, T., Sherwood, R. F. (eds) Biotechnology Handbooks. Plenum Press New York.
Onofri, S., Tosi, S., Persiani, A. M., Maggi, O., Riess, S., Zucconi, L. (1992) Mycological Researches in Victoria Land Terrestial Ecosystems. Proceedings of the 2nd Meeting on Antarctic Biology. Padova, Italy, pp. 19–32.
Petruccioli, M., Fenice, M., Piccioni, P. (1993) Distribution and topology of glucose oxidase activity in the genus Penicillium. Lett. Appl. Microbiol. 17, 285–288.
Potier, P., Drevet, P., Gounot, A. M., Hipkiss, A. (1990) Temperature-dependent changes in proteolytic activities and protein composition in the psychrotrophic bacterium Arthrobacter globiformis S 55. J. Gen. Microbiol. 136, 283–291.
Scandalios, J. G. (1992) Regulation of the antioxidant defence genes Cat and Sod of maize. In: Scandalios, J. G. (ed.), Molecular Biology of Free Radical Scavenging Systems. Cold Spring Harbor Laboratory Press, New York, pp. 117–152.
Schacterle, G. R., Pollack, R. L. (1973) A simplified method for the quantitative assay of small amounts of protein in biologic material. Anal. Biochem. 51, 654–655.
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This work was financially supported by the research program No.I/BiNoZ/statut/.
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Fiedurek, J., Gromada, A., Słomka, A. et al. Catalase Activity in Arctic Microfungi Grown at Different Temperatures. BIOLOGIA FUTURA 54, 105–112 (2003). https://doi.org/10.1556/ABiol.54.2003.1.11
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DOI: https://doi.org/10.1556/ABiol.54.2003.1.11