Abstract
The complete identification of coding sequences in a number of species has led to announce the beginning of the post-genomic era, new tools have become available to study complex phenomena in biological systems. Rapid advances in genomic sequencing and bioinformatics have established the field of genomics to investigate thousands of genes’ activity through mRNA display. However, recent studies have demonstrated a lack of correlation between the transcriptional profiles and the actual protein levels in cells, so investigation of the expressed part of the genome is also required to link genomic data to biological function. It is possible that evolutional development occurred by increasing complexity of regulation processes at the level of RNA and protein molecules instead of simple increase in gene number, so investigation of proteins and protein complexes became important fields of our post-genomic era. Highresolution two-dimensional gels combined with sensitive mass spectrometry can reveal virtually all proteins present in cells opening new insights into functions of cells, tissues and whole organisms.
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Abbreviations
- 2-DE:
-
two-dimensional gel-electrophoresis
- MS:
-
mass spectrometry
- IPG:
-
immobilized pH gradient
- SDS:
-
sodium dodecyl sulphate
- PAGE:
-
polyacrylamide gel-electrophoresis
- TCA:
-
trichlororacetic acid
- CBB:
-
Coomassie Brillant Blue
- CDD:
-
charge-coupled device
- MALDI-TOF:
-
matrix-assisted laser desorption ionization-time of flight
- ESI:
-
electrospray ionization
- PCD:
-
programmed cell death
- DSP:
-
disease-specific protein
- LCM:
-
laser capture microdissection
- SPR-BIA:
-
surface plasmon resonance-biomolecular interaction analysis
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This work was supported by OTKA F030338.
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Mádi, A., Pusztahelyi, T., Punyiczki, M. et al. The Biology of the Post-Genomic ERA: The Proteomics. BIOLOGIA FUTURA 54, 1–14 (2003). https://doi.org/10.1556/ABiol.54.2003.1.1
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DOI: https://doi.org/10.1556/ABiol.54.2003.1.1