Abstract
Mucor genevensis is a dimorphic and homothallic fungal species (Zygomycetes). Ten M. genevensis strains, each strain of the recently described new homothallic species (M. meguroense and M. hachijyoensis) and strains of M. hiemalis and M. piriformis (as outgroups for numerical analysis) were investigated. Five different enzyme systems (CAT, GDH, G6D, MDH and SOD) and five 10-bp random primers were used in isoenzyme and random amplified polymorphic DNA analyses, respectively. The data from these studies were subjected to numerical analyses. Substantial intraspecific variability was detected in M. genevensis with both of the methods applied. Though both the M. meguroense strain and the M. hachi-jyoensis strain revealed characteristic differences, they grouped closer to the homothallic M. genevensis than to the heterothallic M. piriformis and M. hiemalis strains.
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Anné, J., Peberdy, J. F. (1981) Characterisation of interspecific hybrids between Penicillium chryso-genum and P. roqueforti by iso-enzyme analysis. Trans. Br. Mycol. Soc. 77, 401–408.
Beauchamp, C., Fridovich, J. (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal. Biochem. 44, 276–287.
Brewer, G. J., Sing, C. F. (1970) An introduction to isozyme techniques. Academic Press, New York.
Havens, P. L. (1976) Comparative zone electrophoresis and mating experiments in the taxonomy of Mucor hiemalis. Mycotaxon 4, 218–232.
Hesseltine, C. W. (1954) The section Genevensis of the genus Mucor. Mycologia 46, 358–366.
Kanouse, B. B. (1924) The life history of a new homothallic Mucor. Pap. Mich. Acad. Sci. 3, 120–130.
Leach, J., Finkelstein, D. B., Rambosek, J. A. (1986) Rapid miniprep of DNA from filamentous fungi. Fungal Genet. Newslett. 33, 32–33.
Mulvey, M., Vrijenhoek, R. C. (1981) Genetic variation among laboratory strains of the planorbid snail Biomphalaria glabrata. Biochem. Genet. 19, 1169–1182.
Naumov, N. A. (1935) Opredelitel Mukorovych (Mucorales). 2nd revised ed. Bot. Inst. Acad. Sci. USSR, Moscow and Leningrad. 140 pp. (In Russian).
Papp, T., Vágvölgyi, Cs., Kerényi, Z., Nagy, Á., Michailides, T. J. (1997) DNA amplification polymorphisms of Mucor piriformis. Ant. Leeuwenhoek 72, 167–173.
Podani, J. (1993) SYN-TAX-pc, Computer programs for multivariate data analysis in ecology and systematics. Version 5.0. User’s Guide. Scientia Publishing, Budapest.
Schipper, M. A. A. (1973) A study on variability in Mucor hiemalis and related species. Stud. Mycol. 4, 1–40.
Schipper, M. A. A. (1978) On certain species of Mucor with a key to all accepted species. Stud. Mycol. 17, 1–52.
Schipper, M. A. A. (1986) Mating ability and the species concept of the Zygomycetes. In: Rayner, A. D. M., Brasier, C. M., Moore, D. (eds) Evolutionary Biology of Fungi. Cambridge Univ. Press, pp. 261–269.
Sneath, P. H. A., Sokal, R. R. (1973) Numerical taxonomy. W. H. Freeman and Co., San Francisco.
Vágvölgyi, Cs., Papp, T., Palágyi, Zs., Michailides, T. J. (1996) Isozyme variation among isolates of Mucor piriformis. Mycologia 88, 602–607.
Vágvölgyi, Cs., Vastag, M., Acs, K., Papp, T. (1999) Rhizomucor tauricus: a questionable species of the genus. Mycol. Res. 103, 612–618.
Vastag, M., Papp, T., Kasza, Zs., Vágvölgyi, Cs. (1998) Differentiation of Rhizomucor species by means of carbon source utilization and isoenzyme analysis. J. Clin. Microbiol. 36, 2153–2156.
Watanabe, T. (1994) Two new species of homothallic Mucor in Japan. Mycologia 86, 691–695.
Williams, J. G. K., Kubelik, A. R., Rafalski, J. A., Tingey, S. V (1991) Genetic analysis with RAPD markers. In: Bennett, J. W., Lasure, L. L. (eds) More genetic manipulations in fungi. Academic Press, San Diego. pp. 431–439.
Woodbury, W., Spencer, A. K., Stanmann, M. A. (1971) An improved procedure using ferricyanide for detecting catalase isozymes. Anal. Biochem. 44, 301–305.
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Dedicated to Professor Lajos Ferenczy on the occasion of his 70th birthday.
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Vágvölgyi, C., Kasza, Z., Nyilasi, I. et al. Variability of Isozyme and Rapd Markers Among Isolates of Mucor genevensis. BIOLOGIA FUTURA 52, 365–373 (2001). https://doi.org/10.1556/ABiol.52.2001.2-3.21
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DOI: https://doi.org/10.1556/ABiol.52.2001.2-3.21