Abstract
The differentiation and metabolism in the soil-borne saprophytic deuteromycete Trichoderma viride are subject to control by light. We have investigated the effect of illumination of mycelia on the activities of cell-wall synthesizing enzymes β-1,3-glucan synthase and chitin synthase and on the composition of cell walls. After 10 min illumination of dark-grown mycelia with white fluorescent light at 600 lx, a gradual rise in specific activity of membrane-bound enzymes β-1,3-glucan synthase by about 130% and a decrease in specific activity of chitin synthase by about 50% in relation to the dark control were observed. The changes in enzyme activities were caused by de novo synthesis of corresponding poly-saccharide synthases(s) and/or their regulatory components since they were not observed when protein synthesis was blocked with 50 μg/ml cycloheximide. The content of β-1,3-glucan in the cell walls of illuminated mycelia has increased by 35–50% in comparison to the dark control while the content of chitin remained practically unchanged.
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Abbreviations
- PMSF:
-
phenylmethylsulfonyl fluoride
- BSA:
-
bovine serum albumin
- EGTA:
-
ethylene glycol-bis(ß-aminoethyl ether)
- N,N,N′:
-
N′-tetraacetic acid
- DTT:
-
dithiothreitol
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Dedicated to Professor Lajos Ferenczy on the occasion of his 70th birthday.
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Nemčovič, M., Farkaš, V. Cell-Wall Composition and Polysaccharide Synthase Activity Changes Following Photoinduction in Trichoderma viride. BIOLOGIA FUTURA 52, 281–288 (2001). https://doi.org/10.1556/ABiol.52.2001.2-3.12
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DOI: https://doi.org/10.1556/ABiol.52.2001.2-3.12