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Microfluidic Multiple Cell Chip Reactor Filled with Enzyme-Coated Magnetic Nanoparticles — An Efficient and Flexible Novel Tool for Enzyme Catalyzed Biotransformations

Abstract

Biotransformation of L-phenylalanine (L-1a) and five unnatural substrates (rac-1b–f) by phenylalanine ammonia-lyase (PAL) was investigated in a novel microfluidic device (Magne-Chip) that comprises microliter volume reaction cells filled with PAL-coated magnetic nanoparticles (MNPs). Experiments proved the excellent reproducibility of enzymecatalyzed biotransformation in the chip and the excellent reusability of the enzyme layer during 14 h continuous measurement (>98% over 7 repetitive measurements with L-1a). The platform also enabled fully automatic multiparameter measurements with a single biocatalyst loading of about 1 mg PAL-MNP. Computational fluid dynamics (CFD) calculations were used to study the flow field in the chambers and the effect of unintended bubble formation. Optimal flow rate for L-1a reaction and specific activities for rac-1b–f under these conditions were determined.

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Correspondence to Ferenc Ender or László Poppe.

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Ender, F., Weiser, D., Nagy, B. et al. Microfluidic Multiple Cell Chip Reactor Filled with Enzyme-Coated Magnetic Nanoparticles — An Efficient and Flexible Novel Tool for Enzyme Catalyzed Biotransformations. J Flow Chem 6, 43–52 (2016). https://doi.org/10.1556/1846.2015.00036

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  • DOI: https://doi.org/10.1556/1846.2015.00036

Keyword

  • magnetic nanoparticle
  • magnetic chip reactor
  • continuous-flow biotransformation
  • phenylalanine ammonia-lyase
  • unnatural amino acid