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High-Performance Thin-Layer Chromatography Profiling of Jarrah and Manuka Honeys

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Summary

This article presents the findings of an in-depth study on high-performance thin-layer chromatographic (HPTLC) profiling of Jarrah (Eucalyptus marginata) and Manuka (Leptospermum spp.) honeys following a simple one-step solvent extraction process. The study demonstrates that different HPTLC fingerprints are obtained from honeys from varying floral sources and that honeys of the same floral origin present a consistent reproducible HPTLC profile. In addition, the linearity and reproducibility of this technique as well as its ability to detect (accidental or deliberate) contaminations with honeys of different floral sources are demonstrated. The study thus illustrates the usefulness of HPTLC profiling as a potential quality control tool that might complement other analytical techniques used in the authentication of monofloral honeys.

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References

  1. S. Sulaiman, Honey in Traditional and Modern Medicine, CRC Press, Boca Raton, FL, 2013.

  2. E. Mavric, S. Wittmann, G. Barth, P. Henle, Mol. Nutr. Food Res. 52 (2008) 483–489.

    Article  CAS  Google Scholar 

  3. C. Adams, M. Manley-Harris, P.C. Molan, Carbohydr. Res. 344 (2009) 1050–1053.

    Article  CAS  Google Scholar 

  4. L.M. Bang, C. Buntting, P.C. Molan, J. Alternat. Compl. Med. 9 (2003) 267–273.

    Article  Google Scholar 

  5. J. Irish, S. Blair, D.A. Carter, PLoS ONE 6 (2011) e18229.

    Article  CAS  Google Scholar 

  6. Ministry for Primary Industries, Apiculture, Wellington, 2017.

  7. Ministry for Primary Industries, Manuka Honey, 2017; http://www.mpi.govt.nz/growing-and-producing/bees-and-other-insects/manuka-honey/.

  8. C. Locher, J. Neumann, T. Sostaric, J. Planar Chromatogr. 30 (2017) 57–62.

    Article  CAS  Google Scholar 

  9. S. Soares, J.S. Amaral, M.B.P.P. Oliveira, I. Mafra, Comp. Rev. Food Sci. F. (2017), https://doi.org/10.1111/1541-4337.12278.

  10. A.J. Siddiqui, S.G. Musharraf, M.I. Choudhary, Atta-ur-Rahman, Food Chem. 217 (2017) 687–698.

    Article  CAS  Google Scholar 

  11. Z. Jandrić, S.A. Haughey, R.D. Frew, K. McComb, P. Galvin-King, C.T. Elliott, A. Cannavan, Food Chem. 189 (2015) 52–59.

    Article  Google Scholar 

  12. V. Kaskoniene, P.R. Venskutonis, Compr. Rev. Food Sci. Food Saf. 9 (2010) 620–634.

    Article  CAS  Google Scholar 

  13. D. Kasote, A. Ahmad, W. Chen, S. Combrinck, A. Viljoen, Phytochem. Lett. 11 (2015) 326–331.

    Article  CAS  Google Scholar 

  14. P. Ristivojević, F.L. Andrić, J.D. Trifković, I. Vovk, L.Z. Stanisavljević, Z.L. Tešić, D.M. Milojković-Opsenica, J. Chemometrics 28 (2013) 301–310.

    Article  Google Scholar 

  15. A. Puscas, A. Hosu, C. Cimpoiu, J. Chromatogr. A 1272 (2013) 132–135.

    Article  CAS  Google Scholar 

  16. E.S. Chemetsova, I.A. Revelsky, G.E. Morlock, Anal. Bioanal. Chem. 401 (2011) 325–332.

    Article  Google Scholar 

  17. E. Anklam, Food Chem. 63 (1998) 549–562.

    Article  CAS  Google Scholar 

  18. N. Beitlich, T. Lübken, M. Kaiser, L. Ispiryan, K. Speer, J. Agric. Food Chem. 64 (2016) 8886–8891.

    Article  CAS  Google Scholar 

  19. B. Lin, K.M. Loomes, G. Prijic, R. Schlothauer, J.M. Stephens, Food Chem. 225 (2017) 175–180.

    Article  CAS  Google Scholar 

  20. J. Bong, G. Prijic, T.J. Braggins, R.C. Schlothauer, J.M. Stephens, K.M. Loomes, Food Chem. 214 (2017) 102–109.

    Article  CAS  Google Scholar 

  21. N. Beitlich, I. Koelling-Speer, S. Oelschlaegel, K. Speer, J. Agric. Food Chem. 62 (2014) 6435-6444.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. School of Allied Health and CRC for Honey Bee Products, University of Western Australia, Crawley, WA, Australia

    Cornelia Locher

  2. School of Allied Health, University of Western Australia, Crawley, WA, Australia

    Edith Tang & Jonas Neumann

  3. Chromatech Scientific Pty Ltd., Yangebup, WA, 6164, Australia

    Tomislav Sostaric

Authors
  1. Cornelia Locher
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  2. Edith Tang
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  3. Jonas Neumann
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  4. Tomislav Sostaric
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Correspondence to Cornelia Locher.

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Locher, C., Tang, E., Neumann, J. et al. High-Performance Thin-Layer Chromatography Profiling of Jarrah and Manuka Honeys. JPC-J Planar Chromat 31, 181–189 (2018). https://doi.org/10.1556/1006.2018.31.3.1

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  • DOI: https://doi.org/10.1556/1006.2018.31.3.1

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