Abstract
Plant secondary metabolites anthocyanins are considered to play a protective role. In bread wheat (Triticum aestivum L.), anthocyanins can be observed in both adult plants and seedlings. The aim of the current study was to investigate the putative role of anthocyanins present in grains and shoots with respect to the protection of seedlings against drought. For this purpose a set of near isogenic lines (NILs) differing in pericarp and coleoptile colour was used. Water stress was created by artificial shortage of moisture under laboratory conditions. Differences among the lines were observed in a way that the lines with dark-purple grains and coleoptiles (genotype Pp-D1Pp-D1Pp3Pp3Rc-A1Rc-A1Rc-D1Rc-D1) demonstrated a higher seedling drought tolerance than plants with uncoloured pericarp and light-purple coleoptiles (pp-D1pp-D1pp3pp3Rc-A1Rc-A1rc-D1rc-D1). Furthermore, protection of the root system and the shoot was related with the presence of anthocyanins in grains and coleoptiles, respectively.
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References
Arbuzova, V.S., Maystrenko, O.I., Popova, O.M. 1998. Development of near-isogenic lines of the common wheat cultivar ‘Saratovskaya 29’. Cereal Res. Commun. 26:39–46.
Blum, A., Ramaiah, S., Kanemasu, E.T., Paulsen, G.M. 1990. Wheat recovery from drought stress at the tillering stage of development. Field Crop. Res. 24:67–85.
Borghesi, E., González-Miret, M.L., Escudero-Gilete, M.L., Malorgio, F., Heredia, F.J., Meléndez-Martínez, A.J. 2011. Effects of salinity stress on carotenoids, anthocyanins, and colour of diverse tomato genotypes. J. Agric. Food Chem. 59:11676–11682.
Bors, W., Mochel, C., Saran, M. 1994. Flavonoid antioxidants: Rate constants for reactions with oxygen radicals. Methods Enzymol. 234:420–429.
Castellarin, S.D., Pfeiffer, A., Sivilotti, P., Degan, M., Peterlunger, E., Gaspero, G. 2007. Transcriptional regulation of anthocyanin biosynthesis in ripening fruits of grapevine under seasonal water deficit. Plant Cell Environ. 30:1381–1399.
Chalker-Scott, L. 1999. Environmental significance of anthocyanins in plant stress responses. Photochem. Photobiol. 70:1–9.
Daneshmand, F., Arvin, M.J., Kalantari, K.M. 2010. Physiological responses to NaCl stress in three wild species of potato in vitro. Acta Physiol. Plant. 32:91–101.
Fedenko, V.S. 2008. Tsianidyn yak endohennyy khelator ioniv metaliv u korenyakh prorostkiv kukurudzy [Cyanidin as endogenous chelator of metal ions in maize seedling roots.] Ukr. Biokhim. Zh. (Ukr. Biochem. J.) 80:102–106. [in Ukrainian]
Gordeeva, E.I., Shoeva, O.Y., Khlestkina, E.K. 2013. Cold stress response of wheat genotypes having different Rc alleles. Cereal Res. Commun. 41:519–526.
Gordeeva, E.I., Shoeva, O.Y., Khlestkina, E.K. 2015. Marker-assisted development of bread wheat near-isogenic lines carrying various combinations of Pp (purple pericarp) alleles. Euphytica 203:469–476.
Hale, K.L., McGrath, S.P., Lombi, E., Stack, S.M., Terry, N., Pickering, I.J., George, G.N., Pilon-Smits, E.A. 2001. Molybdenum sequestration in Brassica species. A role for anthocyanins? Plant Physiol. 126:1391–1402.
Hale, K.L., Tufan, H.A., Pickering, I.J., George, G.N., Terry, N., Pilon, M., Pilon-Smits, E.A.H. 2002. Anthocyanins facilitate tungsten accumulation in Brassica. Physiol. Plant. 116:351–358.
Ivanova, G.F., Levitskaya, N.G., Shatalova, O.V. 2011. Vliyanie ekstremalnyih proyavleniy klimaticheskih izmeneniy na produktivnost selskohozyaystvennyih kultur [Influence of extreme displays of climatic changes on efficiency of agricultural crops.] Izvestiya of Saratov University. New series. Series: Earth Sciences 11:41–47. [in Russian]
Khlestkina, E.K. 2013. Genes determining the coloration of different organs in wheat. Russ. J. Genet. Appl. Res. 3:54–65.
Khlestkina, E.K., Gordeeva, E.I., Arbuzova, V.S. 2014. Molecular and functional characterization of wheat near-isogenic line ‘i:S29Ra’ having intensive anthocyanin pigmentation of the coleoptile, culm, leaves and auricles. Plant Breed. 133:454–458.
Lo Piero, A.R., Puglisi, I., Rapisarda, P., Petrone, G. 2005. Anthocyanins accumulation and related gene expression in red orange fruit induced by low temperature storage. J. Agric. Food Chem. 53:9083–9088.
Merzlyak, M.N., Melø, T.B., Naqvi, K.R. 2008. Effect of anthocyanins, carotenoids and flavonols on chlorophyll fuorescence excitation spectra in apple fruit: signature analysis, assessment, modelling, and relevance to photoprotection. J. Exp. Bot. 59:349–359.
Nakabayashi, R., Mori, T., Saito, K. 2014. Alternation of flavonoid accumulation under drought stress in Arabidopsis thaliana. Plant Signalling Behav. 9:e29518.
Pandey, P., Ramegowda, V., Senthil-Kumar, M. 2015. Shared and unique responses of plants to multiple individual stresses and stress combinations: physiological and molecular mechanisms. Front. Plant Sci. 6:723
Ploenlap, P., Pattanagul, W. 2015. Effects of exogenous abscisic acid on foliar anthocyanin accumulation and drought tolerance in purple rice. Biologia 70:915–921.
Sanchita, Singh, R., Mishra, A., Dhawan, S.S., Shirke, P.A., Gupta, M.M., Sharma, A. 2015. Physiological performance, secondary metabolite and expression profiling of genes associated with drought tolerance in Withania somnifera. Protoplasma 252:1439–1450.
Solovchenko, A.E., Merzlyak, M.N. 2008. Screening of visible and UV radiation as a photoprotective mechanism in plants. Russ. J. Plant Physiol. 55:719–737.
Steyn, W.J., Wand, S.J.E., Holcroft, D.M., Jacobs, G. 2002. Anthocyanins in vegetative tissues: A proposed unified function in photoprotection. New Phytol. 155:349–361.
Tereshchenko, O.Y., Gordeeva, E.I., Arbuzova, V.S., Börner, A., Khlestkina, E.K. 2012. The D genome carries a gene determining purple grain colour in wheat. Cereal Res. Commun. 40:334–341.
Wang, H., Cao, G., Prior, R.L. 1997. Oxygen radical absorbing capacity of anthocyanins. J. Agric. Food Chem. 45:304–309.
Yudina, R.S., Leonova, I.N., Salina, E.A., Khlestkina, E.K. 2015. Effect of alien genomic introgressions on the osmotic stress resistance of wheat. Russ. J. Genet. Appl. Res. 5:168–173.
Acknowledgements
We thank Ms Galina Generalova (ICG SB RAS) for technical assistant. Olesya Shoeva thanks RFBR (grant No 16-34-60052) for partial support of this study. Elena Gordeeva, Valentina Arbuzova and Elena Khlestkina thank ICG project (No 0324-2015-0005) for financial support.
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Shoeva, O.Y.U., Gordeeva, E.I., Arbuzova, V.S. et al. Anthocyanins Participate in Protection of Wheat Seedlings from Osmotic Stress. CEREAL RESEARCH COMMUNICATIONS 45, 47–56 (2017). https://doi.org/10.1556/0806.44.2016.044
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DOI: https://doi.org/10.1556/0806.44.2016.044