Abstract
Maydis leaf blight (MLB), a serious foliar fungal disease of maize, may cause up to 40% losses in yield. The present studies were undertaken to identify the stable sources of MLB resistance, its inheritance study, and testing of MLB resistance linked markers from diverse background in the Indian adapted tropical maize genotypes. A set of 112 inbred lines were screened under artificially created epiphytotics conditions at three hotspot locations. Analysis across multi-locations revealed significant effects of genotypes and environments, and non-significant effects due to genotypes × environment interaction on disease incidence. A total of 25 inbred lines with stable resistance were identified across multi-locations. Inheritance of resistance was studied in six F1s and two F2s of resistant and susceptible parents. The null hypothesis of segregation of resistance and susceptible for mono and digenic ratios in two F2 populations was rejected by Chi-square test. The non-significant differences among the reciprocal crosses depicted the complete control of nuclear genome for MLB resistance. Partial dominance in F1s and normal distribution pattern in F2s of resistant and susceptible parents suggested polygenic nature of MLB resistance. Correlation studies in F2 populations exhibited significant negative correlation between disease score and days to flowering. Five simple sequence repeats (SSRs) markers, found associated to MLB resistance in different studies were unable to differentiate amongst MLB resistance and susceptible parents in our study. This emphasizes the need of fine mapping for MLB resistance in Indian germplasm. The identified stable sources of resistance and information on inheritance study can be used further in strengthening of resistance breeding against MLB.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Balint-Kurti, P.J., Carson, M.L. 2006a. Analysis of quantitative trait loci for resistance to southern leaf blight in juvenile maize. Phytopathol. 96:221–225.
Balint-Kurti, P.J., Krakowsky, M.D., Jines, M.P., Robertson, L.A., Molnár, T.L., Goodman, M.M., Holland, J.B. 2006b. Identification of quantitative trait loci for resistance to southern leaf blight and days to anthesis in a maize recombinant inbred line population. Phytopathol. 96:1067–1071.
Balint-Kurti, P.J., Zwonitzer, J.C., Wisser, R.J., Carson, M.L., Oropeza-Rosas, M., Holland, J.B., Szalma, S.J. 2007. Precise mapping of quantitative trait loci for resistance to southern leaf blight, caused by Cochliobolus heterostrophus race O, and flowering time using advanced intercross maize lines. Genetics 176:645–657.
Balint-Kurti, P.J., Zwonitzer, J.C., Pe, E., Pea, G., Lee, M., Cardinal, A. 2008. Identification of quantitative trait loci for resistance to southern leaf blight and days to anthesis in two maize recombinant inbred line populations. Phytopathol. 98:315–320.
Belcher, A.R., Zwonitzer, J.C., Cruz, J.S., Krakowsky, M.D., Chung, C.L., Nelson, R., Arellano, C. Balint-Kurti, P.J. 2011. Analysis of quantitative disease resistance to southern leaf blight and of multiple disease resistance in maize, using near-isogenic lines. Theor. Appl. Genet. 124:433–445.
Burnette, D.C., White, D.G. 1985. Inheritance of resistance to Bipolari maydis race O in crosses derived from nine resistant inbred lines of maize. Phytopathol. 75:1195–1200.
Bubeck, D.M., Goodman, M.M., Beavis, W.D., Grant, D. 1993. Quantitative trait loci controlling resistance to gray leaf spot in maize. Crop Sci. 33:838–847.
Byrnes, K.J., Pataky, J.K., White, D.G. 1989. Relationships between yield of three maize hybrids and severity of southern leaf blight caused by race O of Bipolaris maydis. Plant Dis. 73:834–840.
Carson, M.L., Stuber, C.W., Senior, M.L. 2004. Identification and mapping of quantitative trait loci conditioning resistance to southern leaf blight of maize caused by Cochliobolus heterotrophus race O. Phytopathol. 94:862–867.
Craig, J., Fajemisin, J.M. 1969. Inheritance of chlorotic lesion resistance to Helminthosporium maydis in maize. Plant Disease Reporter 53:742–743.
Holley, R.N., Goodman, M.M. 1989. New sources of resistance to southern corn leaf blight from tropical hybrid maize derivatives. Plant Dis. 73:562–564.
Kump, K.L., Holland, J.B., Jung, M.T., Wolters, P., Balint-Kurti, P.J. 2010. Joint analysis of near isogenic and recombinant inbred line populations yields precise positional estimates for QTL. Plant Genome 3:142–153.
Kump, K.L., Bradbury, P.J., Wisser, R.J., Buckler, E.S., Belcher, A.R., Oropeza-Rosas, M.A., Zwonitzer, J.C., Kresovich, S., McMullen, M.D., Ware, D., Balint-Kurti, P.J., Holland, J.B. 2011. Genome-wide association study of quantitative resistance to southern leaf blight in the maize nested association mapping population. Nature Genetics 43:163–168.
Pate, J.B., Harvey, P.H. 1954. Studies on the inheritance of resistance in corn to Helminthosporium maydis leaf spot. Agron. J. 46:442–445.
Payak, M.M., Sharma, R.C. 1983. Disease rating scales in maize in India. In: Techniques of scoring for resistance to important diseases of maize. All India Coordinated Maize Improvement Project. Indian Agriculture Research Institute. New Delhi, India. pp. 1–4.
Saghai-Maroof, M.A., Soliman, K.M., Jorgensen, R.A., Allard, R.W. 1984. Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location and population dymnamics. Proc. Natl Acad. Sci. USA 81:8014–8018.
Smith, D.R., Hooker, A.L. 1973. Monogenic chlorotic-lesion resistance in corn to Helminthosporium maydis. Crop Sci. 13:330–331.
Van Eijnatten, C.L.M. 1961. Susceptibility to leaf blight caused by Cochliobolus heterostrophus in Nigerian varieties of maize. Nature London 191:515–516.
Van Inghelandt, D., Melchinger, A.E., Pier Martinant, J., Stich, B. 2012. Genome-wide association mapping of flowering time and northern corn leaf blight (Setosphaeria turcica) resistance in a vast commercial maize germplasm set. BMC Plant Biol. 12:56.
White, D.G. 1999. Compendium of Corn Diseases, 3rd ed. Amer. Phytopathol. Soc. St. Paul, MN, USA.
Wisser, R.J., Balint-Kurti, P.J., Nelson, R.J. 2006. The genetic architecture of disease resistance in maize: a synthesis of published studies. Phytopathol. 96:120–129.
Wisser, R.J., Kolkman, J.M., Patzoldt, M.E., Holland, J.B., Yu, J., Krakowsky, M., Nelson, R.J., Balint-Kurti, P.J. 2011. Multivariate analysis of maize disease resistance suggests a pleiotropic genetic basis and implicates a GST gene. Proc. Natl Acad. Sci. USA 108:7339–7344.
Zaitlin, D., Demars, S., Ma, Y. 1993. Linkage of rhm, a recessive gene for resistance to southern corn leaf blight, to RFLP marke loci in maize (Zea mays) seedlings. Genome 36:555–564.
Zwonitzer, J.C., Coles, N.D., Krakowsky, M.D., Arellano, C., Holland, J.B., McMullen, M.D., Pratt, R.C., Balint-Kurti, P.J. 2010. Mapping resistance quantitative trait loci for three foliar diseases in a maize recombinant inbred line population-evidence for multiple disease resistance? Phytopathol. 100:72–79.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
About this article
Cite this article
Kumar, B., Hooda, K.S., Gogoi, R. et al. Inheritance Study and Stable Sources of Maydis Leaf Blight (Cochliobolus heterostrophus) Resistance in Tropical Maize Germplasm. CEREAL RESEARCH COMMUNICATIONS 44, 424–434 (2016). https://doi.org/10.1556/0806.44.2016.004
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1556/0806.44.2016.004