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Cereal Research Communications

, Volume 43, Issue 2, pp 204–212 | Cite as

The Analysis of SKP1 Gene Expression in Physiological Male Sterility Induced by Chemical Hybridizing Agent SQ-1 in Wheat (Triticum aestivum L.)

  • M. Y. Wang
  • Y. L. Song
  • S. X. Zhang
  • X. L. Zhao
  • J. W. Wang
  • N. Niu
  • G. S. ZhangEmail author
Physiology

Abstract

Physiological male sterility induced by the chemical hybridizing agent (CHA) overcomes problems of maintenance of sterile lines and restorers. However, the mechanism of sterility is unclear. The process of tapetum of CHA-treated ‘Xi’nong 2611’ at uninucleate, binucleate and trinucleate were compared with control to determine if tapetum varying differently during developmental stages. Tapetal degradation in CHA-treated ‘Xi’nong 2611’ began at late uninucleate stage, somewhat earlier than control plants. Cytological observations indicated that the gradual degradation of the tapetum in CHA-treated ‘Xi’nong 2611’ was initiated and terminated earlier than in the control. These findings implied that CHA-induced male sterility was related to abnormally early tapetal degradation. In order to indicate the role of the SKP1 gene in fertility/sterility in wheat, its expression was assessed in anthers at uninucleate, binucleate and trinucleate stages. SKP1 expression was reduced in the later developmental stages, and there was an obvious decrease from the uninucleate to trinucleate stages. Higher expression of the SKP1 gene occurred in ‘Xi’nong 2611’ compared to CHA-treated ‘Xi’nong 2611’. This implied that SKP1 gene expression was inhibited during the fertility transformation process and was related to transformation from fertility to sterility. Moreover, the results from this study suggest that SKP1 plays an essential role of conducting fertility in physiological male sterility.

Keywords

wheat CHA tapetum RT-PCR 

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© Akadémiai Kiadó, Budapest 2015

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • M. Y. Wang
    • 1
  • Y. L. Song
    • 1
  • S. X. Zhang
    • 2
  • X. L. Zhao
    • 1
  • J. W. Wang
    • 1
  • N. Niu
    • 1
  • G. S. Zhang
    • 1
    Email author
  1. 1.Key Laboratory of Crop Heterosis of Shaanxi Province / National Yangling Agricultural Biotechnology and Breeding Center / Yangling Branch of State Wheat Improvement Centre / Wheat Breeding Engineering Research CenterMinistry of Education / Northwest A&F UniversityYangling, ShaanxiPR China
  2. 2.Institute of Crop ScienceNingxia Academy of Agricultural SciencesNingxiaPR China

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