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Cereal Research Communications

, Volume 42, Issue 3, pp 514–524 | Cite as

Development of Endosperm Cells and Starch Granules in Common Wheat

  • Y. P. Jing
  • D. T. Liu
  • X. R. Yu
  • F. Xiong
  • D. L. Li
  • Y. K. Zheng
  • Y. F. Hao
  • Y. J. Gu
  • Z. WangEmail author
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Abstract

The objective of the present study was to understand the developmental regularity of wheat endosperm cells at different Days After Pollination (DAP) using microscopic and histochemical methods. Resin semi-thin sections of the endosperm and the enzymatically dissociated Starchy Endosperm Cells (SECs) were observed under a light microscope. The results showed that: (1) SECs were irregular-shaped and had two types of starch granules: large oval-shaped A-type starch granules and small spherical B-type starch granules. (2) The growth shape of SECs was referred to as S-curve and the fastest cell growth period was at 16–24 DAP. (3) The largest increase and growth of A-type starch granules were mainly at 4–16 DAP. B-type starch granules increased rapidly after 16 DAP and made up over 90% of the total starch granules in SEC during the late stage of endosperm development. (4) The nuclei of SEC deformed and degenerated during the middle and late stages of endosperm development and eventually disappeared. However, starch granules still increased and grew after the cell nuclei had degenerated. The investigations showed the development regularity of starch endosperm cells and starch granules, thereby improving the understanding of wheat endosperm development.

Keywords

starch endosperm cell starch granule wheat 

Abbreviations

DAP

Days After Pollination

PCD

Programmed Cell Death

SD

Standard Deviation

SEC

Starch Endosperm Cell

SEM

Scanning Electron Microscopy

TTC

2,3,5-Triphenyl-2H-Tetrazolium Chloride

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References

  1. Becraft, P.W. 2001. Cell fate specification in the cereal endosperm. Semin. Cell Dev. Biol. 12:387–394.CrossRefGoogle Scholar
  2. Becraft, P.W., Gutierrez, M.J. 2012. Endosperm development: dynamic processes and cellular innovations underlying sibling altruism. WIREs Dev. Biol. 1:579–593.CrossRefGoogle Scholar
  3. Chen, Y.F., Zhang, J., Xie, P.S., Zhou, W.D., Chen, J.M., Wei, C.X. 2012. Programmed cell death in wheat starchy endosperm during kernel development. Afr. J. Agr. Res. 7:6533–6540.CrossRefGoogle Scholar
  4. Hong, S.Y., Park, J.H., Cho, S.H., Yang, M.S., Park, C.M. 2011. Phenological growth stages of Brachypodium distachyon: codification and description. Weed Res. 51:612–620.CrossRefGoogle Scholar
  5. Liu, Z., Wang, L.L., Zhou, W.D., Chen, Y.F., Wang, Z. 2011. The surface of the geometric characteristics analysis for rice endosperm starch granules by using Image J. J. of Chinese Electron Microscopy Society 30:466–471. (in Chinese with English abstract)Google Scholar
  6. Olsen, O.A. 1998. Endosperm developments. Plant Cell 10:485–488.CrossRefGoogle Scholar
  7. Olsen, O.A. 2004. Nuclear endosperm development in cereals and Arabidopsis thaliana. Plant Cell 16: 215–227.CrossRefGoogle Scholar
  8. Sabelli, P.A., Larkins, B.A. 2009a. The development of endosperm in grasses. Plant Physiol. 149:14–26.CrossRefGoogle Scholar
  9. Sabelli, P.A., Larkins, B.A. 2009b. The contribution of cell cycle regulation to endosperm development. Sex. Plant Reprod. 22:207–219.CrossRefGoogle Scholar
  10. Sabelli, P.A., Liu, Y., Dante, R.A., Lizarraga, L.E., Nguyen, H.N., Brown, S.W., Klingler, J.P., Yu, J., Labrant, E., Layton, T.M., Feldman, M., Larkins, B.A. 2013. Control of cell proliferation, endore duplication, cell size, and cell death by the retinoblastoma-related pathway in maize endosperm. Proc. Natl Acad. Sci. USA 110:1827–1836.CrossRefGoogle Scholar
  11. Uhlmann, N.K., Beckles, D.M. 2010. Storage products and transcriptional analysis of the endosperm of cultivated wheat and two wild wheat species. J. Appl. Genet. 51:431–447.CrossRefGoogle Scholar
  12. Wang, W.H., Guo, W.S., Fang, M.K., Feng, C.N., Zhu, X.K., Peng, Y.X. 2003. Endosperm cell proliferating and grain filling dynamics in wheat. Acta Agronomica Sinica 29:779–784. (in Chinese with English abstract)Google Scholar
  13. Wang, Z., Gu, Y.J., Wang, H.H., Zheng, Y.K., Chen, Y.H., Zhang, W.H., Wu, X.M. 2012. Investigation of the wheat endosperm cell development. Highlights of Sciencepaper Online 5:1815–1832. (in Chinese with English abstract)Google Scholar
  14. Wei, C.X., Zhang, J., Chen, Y.F., Zhou, W.D., Xu, B., Wang, Y.P., Chen, J.M. 2010. Physicochemical properties and development of wheat large and small starch granules during endosperm development. Acta Physiol. Plant. 32:905–916.CrossRefGoogle Scholar
  15. Yang, J.C., Zhang, J.H., Huang, Z.L., Wang, Z.Q., Zhu, Q.S., Liu, L.J. 2002. Correlation of cytokinin levels in the endosperms and roots with cell number and cell division activity during endosperm development in rice. Annals of Botany 90:369–377.CrossRefGoogle Scholar
  16. Young, T.E., Gallie, D.R. 1999. Analysis of programmed cell death in wheat endosperm reveals differences in endosperm development between cereals. Plant Mol. Biol. 39:915–926.CrossRefGoogle Scholar
  17. Young, T.E., Gallie, D.R. 2000. Programmed cell death during endosperm development. Plant Mol. Biol. 44:283–301.CrossRefGoogle Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2014

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Y. P. Jing
    • 1
  • D. T. Liu
    • 1
  • X. R. Yu
    • 1
  • F. Xiong
    • 1
  • D. L. Li
    • 1
  • Y. K. Zheng
    • 1
  • Y. F. Hao
    • 1
  • Y. J. Gu
    • 1
  • Z. Wang
    • 1
    Email author
  1. 1.Key Laboratories of Crop Genetics and Physiology of the Jiangsu Province and Plant Functional Genomics of the Ministry of EducationYangzhou UniversityYangzhouChina

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