Cereal Research Communications

, Volume 41, Issue 4, pp 573–582 | Cite as

Genetic Variation of Wheat Landraces from China as Revealed by Gliadin and Microsatellite Markers

  • X. J. Li
  • X. Xu
  • X. Q. Li
  • X. M. Yang
  • W. H. Liu
  • L. H. LiEmail author
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A reborn interest has occurred during the last decade toward wheat landraces for broadening genetic basis of modern wheat cultivars. The investigation of molecular traits within and between existing landraces of wheat can help scientists to develop appropriate strategies for their efficient maintenance and exploitation. The present study dealt with the gliadin characterization of forty-seven wheat landraces collected from wheat mainly planted areas of China, each of which was represented by a sample of at least 43 individuals. Twelve accessions selected on the basis of gliadin analysis were investigated further using 21 SSR markers. The results proved that landraces of wheat are a mixture of variable individuals genetically distinguishable from each other. Twelve of the analyzed 47 accessions were observed to be homogeneous, while 35 (74.5%) of them were heterogeneous in their gliadin composition. In total, 122 gliadin pattern were observed. On average, 10.1% (Gst) of the total variation arose from differentiation among regions, and 89.9% was attributed to within-region variation. Furthermore, nineteen of the 21 SSR markers were polymorphic across all the populations. The total number of the amplified DNA products was 110, with a mean of 6.11 alleles per locus. The values of genetic diversity within each landrace population varied from 0.006 to 0.351. Implications for the management of this valuable genetic resource are discussed.


genetic diversity gliadin microsatellite wheat landrace 


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  1. Amirouche, R., Misset, M.T. 2003. Hordein polymorphism in diploid and tetraploid mediterranean populations of the Hordeum murinum L. complex. Plant Syst. Evol. 242:83–99.CrossRefGoogle Scholar
  2. Caballero, L., Martýn, L.M., Alvarez, J.B. 2004. Variation and genetic diversity for gliadins in Spanish spelt wheat accessions. Genet. Resour. Crop Evol. 51:679–686.CrossRefGoogle Scholar
  3. Chwedorzewska, K.J., Bednarek, P.T., Puchalski, J. 2002. Studies on changes in specific rye genome regions due to seed aging and regeneration. Cell Mol. Biol. Lett. 7:569–576.PubMedGoogle Scholar
  4. Gregová, E., Hermuth, J., Kraic, J., Dotlačil, L. 1999. Protein heterogeneity in European wheat landraces and obsolete cultivars. Genet. Resour. Crop Evol. 46:521–528.CrossRefGoogle Scholar
  5. He, D.X., Li, H.J., Xu, S.C., Duan, X.Y., Zhou, Y.L., Li, L.H. 2007. Reaction to powdery mildew and stripe rust in related species and landraces of wheat. Genet. Resour. Crop Evol. 54:213–219.CrossRefGoogle Scholar
  6. Hoisington, D., Khairallah, M., Reeves, T., Ribaut, J.M., Skovmand, B., Taba, S., Warburton, M. 1999. Plant genetic resources: What can they contribute toward increased crop productivity? Proc. Natl. Acad. Sci. USA 96:5937–5943.CrossRefGoogle Scholar
  7. Huang, X.Q., Börner, A., Röder, M.S., Ganal, M.W. 2002. Assessing genetic diversity of wheat (Triticum aestivum L.) germplasm using microsatellite markers. Theor. Appl. Genet. 105:699–707.CrossRefGoogle Scholar
  8. Li, X.J., Xu, X., Yang, X.M., Li, X.Q., Liu, W.H., Gao, A.N., Li, L.H. 2012. Genetic diversity of the wheat landrace Youzimai from different geographic regions investigated with morphological traits, seedling resistance to powdery mildew, gliadin and microsatellite markers. Cereal Res. Commun. 40:95–106.CrossRefGoogle Scholar
  9. Liu, Y., Xiong, Z.Y., He, Y.G., Shewry, P.R., He, G.Y. 2007. Genetic diversity of HMW glutenin subunit in Chinese common wheat (Triticum aestivum L.) landraces from Hubei province. Genet. Resour. Crop Evol. 54:865–874.CrossRefGoogle Scholar
  10. Metakovsky, E.V., Branlard, G. 1998. Genetic diversity of French common wheat germplasm based on gliadin alleles. Theor. Appl. Genet. 96:209–218.CrossRefGoogle Scholar
  11. Metakovsky, E.V., Novoselskaya, A.Yu. 1991. Gliadin allele identification in common wheat. I. Methodological aspects of the analysis of gliadin patterns by one-dimensional polyacrylamide gel electrophoresis. J. Genet. Breed. 5:317–324.Google Scholar
  12. Nevo, E., Pagnotta, M.A., Beiles, A., Poreeddu, E. 1995. Wheat storage proteins: glutenin DNA diversity in wild emmer wheat, Triticum dicoccoides, in Israel and Turkey. 3. Environmental correlates and allozymic associations. Theor. Appl. Genet. 91:415–420.CrossRefGoogle Scholar
  13. Obert, D.E., Fritz, A.K., Moran, J.L., Singh, S., Rudd, J.C., Menz, M.A. 2005. Identification and molecular tagging of a gene from PI 289824 conferring resistance to leaf rust (Puccinia triticina) in wheat. Theor. Appl. Genet. 110:1439–1444.CrossRefGoogle Scholar
  14. Pagnotta, M.A., Impiglia, A., Tanzarella, O.A., Nachit, M.M., Porceddu, E. 2004. Genetic variation of the durum wheat landrace Haurani from different agro-ecological regions. Genet. Resour. Crop Evol. 51:863–869.CrossRefGoogle Scholar
  15. Pestsova, E., Röder, M. 2002. Microsatellite analysis of wheat chromosome 2D allows the reconstruction of chromosomal inheritance in pedigrees of breeding programmes. Theor. Appl. Genet. 106:84–91.CrossRefGoogle Scholar
  16. Powell, W., Morgante, M., Andre, C., Hanafey, M., Vogel, J., Tingey, S., Rafalski, A. 1996. The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Mol. Breeding 2:225–238.CrossRefGoogle Scholar
  17. Routray, P., Basha, O., Garg, M., Singh, N.K., Dhaliwal, H.S. 2007. Genetic diversity of landraces of wheat (Triticum aestivum L.) from hilly areas of Uttaranchal, India. Genet. Resour. Crop Evol. 54:1315–1326.CrossRefGoogle Scholar
  18. Saghai Maroof, M.A., Soliman, K.M., Jorgensen, R.A., Allard, R.W. 1984. Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proc. Natl. Acad. Sci. USA 81:8014–8018.CrossRefGoogle Scholar
  19. Wu, J., Yang, X., Wang, H., Li, H., Li, L., Li, X., Liu, W. 2006. The introgression of chromosome 6P specifying for increased numbers of florets and kernels from Agropyron cristatum into wheat. Theor. Appl. Genet. 114:13–20.CrossRefGoogle Scholar
  20. Zhang, L., Li, H., Wang, H., Li, L. 2007. Genetic diversification of the Chinese wheat landrace Mazhamai as revealed by morphological characteristics, seed storage proteins, and microsatellite markers. Can. J. Plant Sci. 87:763–777.CrossRefGoogle Scholar
  21. Zillman, R.R., Bushuk, W. 1979a. Wheat cultivar identification by gliadin electrophoregrams. II. Effects of environmental and experimental factors on the gliadin electrophoregrams. Can. J. Plant Sci. 59:281–286.CrossRefGoogle Scholar
  22. Zillman, R.R., Bushuk, W. 1979b. Wheat cultivar identification by gliadin electrophoregrams. III. Catalogue of electrophoregram formulas of Canadian wheat cultivars. Can. J. Plant Sci. 59:287–298.CrossRefGoogle Scholar

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© Akadémiai Kiadó, Budapest 2013

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • X. J. Li
    • 1
    • 2
  • X. Xu
    • 1
    • 3
  • X. Q. Li
    • 1
  • X. M. Yang
    • 1
  • W. H. Liu
    • 1
  • L. H. Li
    • 1
    Email author
  1. 1.The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI)Institute of Crop Sciences, Chinese Academy of Agricultural SciencesBeijingChina
  2. 2.School of Life Science and TechnologyHenan Institute of Science and TechnologyXinxiangChina
  3. 3.Department of Life Sciences and TechnologyXinxiang UniversityXinxiangChina

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