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Cereal Research Communications

, Volume 41, Issue 4, pp 573–582 | Cite as

Genetic Variation of Wheat Landraces from China as Revealed by Gliadin and Microsatellite Markers

  • X. J. Li
  • X. Xu
  • X. Q. Li
  • X. M. Yang
  • W. H. Liu
  • L. H. LiEmail author
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Abstract

A reborn interest has occurred during the last decade toward wheat landraces for broadening genetic basis of modern wheat cultivars. The investigation of molecular traits within and between existing landraces of wheat can help scientists to develop appropriate strategies for their efficient maintenance and exploitation. The present study dealt with the gliadin characterization of forty-seven wheat landraces collected from wheat mainly planted areas of China, each of which was represented by a sample of at least 43 individuals. Twelve accessions selected on the basis of gliadin analysis were investigated further using 21 SSR markers. The results proved that landraces of wheat are a mixture of variable individuals genetically distinguishable from each other. Twelve of the analyzed 47 accessions were observed to be homogeneous, while 35 (74.5%) of them were heterogeneous in their gliadin composition. In total, 122 gliadin pattern were observed. On average, 10.1% (Gst) of the total variation arose from differentiation among regions, and 89.9% was attributed to within-region variation. Furthermore, nineteen of the 21 SSR markers were polymorphic across all the populations. The total number of the amplified DNA products was 110, with a mean of 6.11 alleles per locus. The values of genetic diversity within each landrace population varied from 0.006 to 0.351. Implications for the management of this valuable genetic resource are discussed.

Keywords

genetic diversity gliadin microsatellite wheat landrace 

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© Akadémiai Kiadó, Budapest 2013

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • X. J. Li
    • 1
    • 2
  • X. Xu
    • 1
    • 3
  • X. Q. Li
    • 1
  • X. M. Yang
    • 1
  • W. H. Liu
    • 1
  • L. H. Li
    • 1
    Email author
  1. 1.The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI)Institute of Crop Sciences, Chinese Academy of Agricultural SciencesBeijingChina
  2. 2.School of Life Science and TechnologyHenan Institute of Science and TechnologyXinxiangChina
  3. 3.Department of Life Sciences and TechnologyXinxiang UniversityXinxiangChina

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