Monitoring of Transcriptional Responses in Roots of Six Wheat Cultivars During Mild Drought Stress
In order to study drought stress responses in roots during moderate water shortage, our group has designed an oligonucleotide microarray containing probes for genes annotated as either stress-related or general ones to examine six wheat cultivars. These include two drought-tolerant and four drought-sensitive genotypes, having different root morphology either during optimal or water-deficit stress conditions. After a two-week-long water withdrawal, no significant changes were detected in root weights but various trends were observed in the cultivars. These tendencies showed relevant correlations with expression kinetics of genes encoding proteins related to root growth and development. On the other hand, probes indicating variation in distinct genotypes mainly belonged to the group of stress-related genes which encode proteins that contribute to carbohydrate remodeling, osmoprotectant accumulation, amino acid metabolism, defense against oxidative stress, and regulation of transcription. Hence, these data allow us to focus on major metabolic processes involved in drought adaptation as well as provide a wide selection of candidate genes for further detailed studies.
Keywordsoligonucleotide-microarray wheat root drought transcriptome changes
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- Benjamini, Y., Hochberg, Y. 1995. Controlling the false discovery rate: A practical and powerful approach to multiple testing. J. of the Royal Statistical Society: Series B 57:289–300.Google Scholar
- Coram, T.E., Brown-Guedira, G., Chen, X.M. 2008. Using transcriptomics to understand the wheat genome. CAB Reviews: Perspectives in Agriculture, Veterinary Science, Nutrition and Natural Resources 3. No. 083.Google Scholar
- Gallé, Á., Csiszár, J., Secenji, M., Guóth, A., Cseuz, L., Tari, I., Györgyey, J., Erdei, L. 2009. Glutathione transferase activity and expression patterns during grain filling in flag leaves of wheat genotypes differing in drought tolerance: Response to water deficit. J. Plant Physiol. 166:1878–1891.CrossRefGoogle Scholar
- Guóth, A., Tari, I., Gallé, Á., Csiszár, J., Pécsváradi, A., Cseuz, L., Erdei, L. 2009. Comparison of the drought stress responses of tolerant and sensitive wheat cultivars during grain filling: Changes in flag leaf photosynthetic activity, ABA levels, and grain yield. J. of Plant Growth Regulation 28:167–176.CrossRefGoogle Scholar
- Sečenji, M., Lendvai, Á., Miskolczi, P., Kocsy, G., Gallé, Á., Szűcs, A., Hoffmann, B., Sárvári, É., Schweizer, P., Stein, N., Dudits, D., Györgyey, J. 2010a. Differences in root functions during long-term drought adaptation: Comparison of active gene sets of two wheat genotypes. Plant Biol. 12:871–882.CrossRefGoogle Scholar
- Szűcs, A., Jäger, K., Jurca, M.E., Fábián, A., Bottka, S., Zvara, Á., Barnabás, B., Fehér, A. 2010. Histological and microarray analysis of the direct effect of water shortage alone or combined with heat on early grain development in wheat (Triticum aestivum). Physiologia Plantarum 140:174–188.CrossRefGoogle Scholar
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