Relative Nitrogen Translocation and Accumulation in Grain of Winter Wheat Cultivars


Field experiments with 12 winter wheat cultivars differing in N grain concentration were carried out during the vegetative years 2004/05 and 2005/06 using three variants of N fertilization (N1 = 0 kg N ha−1, N2 = 100 kg N ha−1, N3 = 200 kg N ha−1). The aim was to determine the effect of cultivar, N fertilization and weather conditions of the respective years on the relative N translocation and grain quality. Biomass N concentration at the stages of heading (Nbh) and maturity (Nbm), grain N concentration (Ng) and straw N concentration (Nv) were determined. The decrease of N concentration in vegetative biomass during grain filling Nv / Nbh was denoted as a relative N translocation — RNT and ratio Ng / Nv as a relative N accumulation — RNA. Low differences in Nbh and Nbm were observed between years and significant difference in Ng and Nv was due to different RNT and RNA efficiency. In the year with low RNT efficiency, Nv was high and Ng and RNA low. N fertilization increased biomass N concentration, more in the vegetative organs than generative ones. Higher N rates decreased efficiency of fertilization which was expressed in RNT and RNA decrease. In the year with low RNT efficiency, the decrease of fertilizer efficiency had a multiplier effect. Cultivars have differed in Nv, Ng, RNT and RNA but their response to N fertilization was similar. Only some cultivars (Zlatka, Renan and Tamaro) possessed N grain concentration answering food end-use without N fertilization but only in the year favourable for N translocation. Certain cultivars (Sonata and Malyska) did not have food quality at all, not even at rate 200 kg N ha−1. Straw N concentration depended strongly on the cultivar efficiency of decreasing N concentration in vegetative biomass during grain filling (RNT). On the contrary, a negative relationship was observed between straw N concentration and Nbh. Cultivars with high RNT efficiency had low Nv, high Ng and high RNA. Possibilities of utilization RNT and RNA as selection criterions on high Ng were recommended.


  1. Arduini, I., Masoni, A., Ercole, L., Mariotti, M. 2006. Grain yield, and dry matter and nitrogen accumulation and remobilization in durum wheat as affected by variety and seeding rate. Eur. J. Agron. 25:309–318.

    Article  Google Scholar 

  2. Aynehband, A., Moezi, A. A., Sabet, M. 2010. Agronomic assessment of grain yield and nitrogen loss and gain of old and modern wheat cultivars under warm climate. African J. of Agricult. Res. 5:222–229.

    Google Scholar 

  3. Barbottin, A., Lecompte, C., Bouchard, C., Jeuffroy, M.H. 2005. Nitrogen remobilization during grain filling in wheat: Genotypic and environmental effects. Crop Sci. 45:1141–1150.

    Article  Google Scholar 

  4. Calderini, D.F., Torres-León, S., Slafer, G.A. 1995. Consequences of wheat breeding on nitrogen and phosphorus yield, grain nitrogen and phosphorus concentration and associated traits. Ann. Bot. 76:315–322.

    Article  Google Scholar 

  5. Cartelle, J., Pedro, A., Savin, R., Slafer, G.A. 2006. Grain weight responses to postanthesis spikelet-trimming in an old and modern wheat under Mediterranean conditions. Eur. J. Agron. 25:365–371.

    Article  Google Scholar 

  6. Dordas, Ch. 2009. Dry matter, nitrogen and phosphorus accumulation, partitioning as affected by N and P fertilization and source-sink relations. Eur. J. Agronomy 30:129–139.

    CAS  Article  Google Scholar 

  7. Ercoli, L., Arduini, I., Mariotti, A., Masoni, A. 2010. Post-anthesis dry matter and nitrogen dynamics in durum wheat as affected by nitrogen and temperature during grain filling. Cereal Res. Commun. 38:294–303.

    Article  Google Scholar 

  8. Ercoli, L., Lulli, L., Mariotti, A., Masoni, A., Arduini, I. 2008. Post-anthesis dry matter and nitrogen dynamics in durum wheat as affected by nitrogen supply and soil water availability. Eur. J. Agron. 28:138–147.

    CAS  Article  Google Scholar 

  9. Kade, M., Barneix, A.J., Olmos, S. 2005. Nitrogen uptake and remobilization in tetraploid “Langdon” durum wheat and recombinant substitution line with the high grain protein gene Gpc-B1. Plant Breed. 124:343–349.

    CAS  Article  Google Scholar 

  10. Moll, R.H., Kamprath, E.J., Jackson, W.A. 1982. Analysis and interpretation of factors which contribute to efficiency of nitrogen utilization. Agron. J. 74:562–564.

    Article  Google Scholar 

  11. Przulj, N., Momcilovic, V. 2001. Genetic variation for dry matter and nitrogen accumulation and translocation in two rowed spring barley. I. Dry matter translocation. Eur. J. Agron. 15:129–139.

    Article  Google Scholar 

  12. Tashir, I.S.A., Nakata, N. 2005. Remobilization of nitrogen and carbohydrate from stems of bread wheat in response to heat stress during grain filling. J. Agron. Crop Sci. 191:106–115.

    Article  Google Scholar 

  13. Uzik, M., Zofajova, A. 2000. Chlorophyll and nitrogen content in leaves of winter wheat at different genotypes and fertilization. Rostlinná výroba 46:237–244.

    Google Scholar 

  14. Uzik, M., Zofajova, A. 2006. Translocation and accumulation of dry matter in winter wheat genotypes. Cereal Res. Commun. 34:1013–1020.

    Article  Google Scholar 

  15. Uzik, M., Zofajova, A. 2009. Response of winter wheat cultivars to N fertilization in grain yield and its components. Pol’nohospodárstvo (Agriculture) 55:171–182.

    CAS  Google Scholar 

  16. Uzik, M., Zofajova, A. 2011. Progress in accumulation and translocation of nitrogen in winter wheat cultivars released in the years 1921–2003. Cereal Res. Commun. (in press)

  17. Xu, Z.Z., Yu, Z.W., Wang, D. 2006. Nitrogen translocation in wheat plants under soil water deficit. Plant and Soil 280:291–303.

    CAS  Article  Google Scholar 

  18. Xu, Z.Z., Yu, Z.W., Wang, D., Zhang, Y.L. 2005. Nitrogen accumulation and translocation for winter wheat under different irrigation regimes. J.Agron. Crop Sci. 191:439–449.

    CAS  Article  Google Scholar 

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Communicated by J. Kubat

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Uzik, M., Zofajova, A. & Bolvanský, M. Relative Nitrogen Translocation and Accumulation in Grain of Winter Wheat Cultivars. CEREAL RESEARCH COMMUNICATIONS 40, 285–295 (2012).

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  • N fertilization
  • winter wheat
  • cultivars
  • relative N translocation
  • relative N accumulation