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Cereal Research Communications

, Volume 39, Issue 1, pp 88–99 | Cite as

Characterization of wheat yellow rust resistance gene Yr17 using EST-SSR and rice syntenic region

  • J. Q. Jia
  • G. R. Li
  • C. Liu
  • M. P. Lei
  • Z. J. YangEmail author
Open Access
Pathology

Abstract

Wheat yellow rust resistance gene Yr17 was originated from the wheat-Aegilops ventricosa introgression, and still effective on the adult plant in Southern China. The previous studies located the gene Yr17 on the translocation of 2NS-2AS using the molecular and cytological markers. In the present study, we screened new PCR-based markers to map the gene Yr17 region from the investigation of a segregating 120 F2 population. All markers including four EST-PCR markers, a SCAR (sequence characterized amplified region) and a PLUG (PCR based landmark unique gene) marker specific to Yr17 gene were mapped on the chromosome 2AS, and located on the chromosomal deletion bin 2AS5-0.8–1.00 region. Based on the wheat-rice collinearity, we found that the sequences of the Yr17 gene linked markers were comparatively matched at rice chromosome 4 and chromosome 7. However, the identified closely linked genomic sequence of Yr17 gene is most likely collinear with genomic region of rice chromosome 4. The newly produced PCR based markers closely linked to Yr17 gene will be useful for the marker-assisted selection in wheat breeding for rust resistance.

Keywords

EST SCAR markers wheat yellow rust rice synteny 

References

  1. Altschul, S.F., Madden, T.L., Schäffer, A.A., Zhang, J., Zhang, Z., Miller, W., Lipman, D.J. 1997. Anew generation of protein database search programs. Nucleic Acids Res. 25:3389–3402.CrossRefGoogle Scholar
  2. Bariana, H.S., McIntosh, R.A. 1993. Cytogenetic studies in wheat. XV. Location of rust resistance genes in VPM1 and their genetic linkage with other disease resistance genes in chromosome 2A. Genome 36:476–482.CrossRefGoogle Scholar
  3. Bariana, H.S., McIntosh, R.A. 1994. Characterization and origin of rust and powdery mildew resistance genes in VPM1 wheat. Euphytica 76:53–61.CrossRefGoogle Scholar
  4. Bartos, P., Ovesna, J., Hanzalova, A., Chrpova, J., Dumalasova, V., Skorpik, M., Sip, V. 2004. Presence of a translocation from Aegilops ventricosa in wheat cultivars registered in the Czech Republic. Czech J. Genet. Plant Breed. 40:31–35.CrossRefGoogle Scholar
  5. Błaszczyk, L., Goyeau, H., Huang, X.Q., Röder, M., Stepien, L., Chelkowski, J. 2004. Identifying leaf rust resistance genes and mapping gene Lr37 on the microsatellite map of wheat. Cell. Mol. Biol. Lett. 9:869–878.PubMedGoogle Scholar
  6. Carollo, V., Matthews, D.E., Lazo, G.R., Blake, T.K., Hummel, D.D., Lui, N., Hane, D.L., Anderson, O.D. 2005. GrainGenes 2.0. an improved resource for the small-grains community. Plant Physiol. 139:643–651.CrossRefGoogle Scholar
  7. Chantret, N., Cenci, A., Sabot, F., Anderson, O., Dubcovsky, J. 2004. Sequencing of the Triticum monococcum hardness locus reveals good microcolinearity with rice. Mol. Genet. Genomics 271:377–386.CrossRefGoogle Scholar
  8. Conley, E.J., Nduati, V., Gonzalez-Hernandez, J.L., Mesfin, A., Trudeau-Spanjers, M., Chao, S., Lazo, G.R., Hummel, D.D., Anderson, O.D., Qi, L.L., Gill, B.S., Echalier, B., Linkiewicz, A.M., Dubcovsky, J., Akhunov, E.D., Dvorák, J., Peng, J.H., Lapitan, N.L., Pathan, M.S., Nguyen, H.T., Ma, X.F., Miftahudin, G.J.P., Greene, R.A., Sorrells, M.E., Hossain, K.G., Kalavacharla, V., Kianian, S.F., Sidhu, D., Dilbirligi, M., Gill, K.S., Choi, D.W., Fenton, R.D., Close, T.J., McGuire, P.E., Qualset, C.O., Anderson, J.A. 2004. A 2600-locus chromosome bin map of wheat homoeologous group 2 reveals interstitial gene-rich islands and colinearity with rice. Genetics 168:625–637.CrossRefGoogle Scholar
  9. Devos, K.M. 2005. Updating the ‘Crop Circle’. Curr. Opin. Plant Biol. 8:155–162.CrossRefGoogle Scholar
  10. Fedak, G. 1999. Molecular aids for integration of alien chromatin through wide crosses. Genome 42:584–591.Google Scholar
  11. Francki, M., Carter, M., Ryan, K., Hunter, A., Bellgard, M., Appels, R. 2004. Comparative organization of wheat homoeologous group 3S and 7L using wheat-rice synteny and identification of potential markers for genes controlling xanthophyll content in wheat. Funct. Integr. Genomics 4:118–130.CrossRefGoogle Scholar
  12. Friebe, B., Jiang, J.M., Raupp, W.J., McIntosh, R.A., Gill, B.S. 1996. Characterization of wheat-alien translocations conferring resistance to diseases and pests: current status. Euphytica 91:59–87.CrossRefGoogle Scholar
  13. Gale, M.D., Devos, K.M. 1998. Comparative genetics in the grasses. Proc. Natl. Acad. Sci. USA 95:1971–1974.CrossRefGoogle Scholar
  14. Helguera, M., Khan, I., Kolmer, J., Lijavetzky, D., Li, Z.Q., Dubcovsky, J. 2003. PCR assays for the Lr37-Yr17-Sr38 cluster of rust resistance genes and their use to develop isogenic hard red spring wheat line. Crop Sci. 43:1839–1847.CrossRefGoogle Scholar
  15. Ishikawa, G., Nakamura, T., Ashida, T., Saito, M., Nasuda, S., Endo, T.R., Wu, J., Matsumoto, T. 2009. Localization of anchor loci representing five hundred annotated rice genes to wheat chromosomes using PLUG markers. Theor. Appl. Genet. 118:499–514.CrossRefGoogle Scholar
  16. Jahier, J., Abelard, P., Tanguy, A.M., Dedryver, F., Rivoal, R., Khatkar, S., Bariana, H.S. 2001. The Aegilops ventricosa segment on chromosome 2AS of the wheat cultivar ‘VPM1’ carries the cereal cyst nematode resistance gene Cre5. Plant Breeding 120:125–128.CrossRefGoogle Scholar
  17. Kolmer, J.A. 1996. Genetics of resistance to wheat leaf rust. Annu. Rev. Phytopathol. 34:435–455.CrossRefGoogle Scholar
  18. Lagudah, E., McFadden, H., Singh, R., Huerta-Espino, J., Bariana, H., Spielmeyer, W. 2006. Molecular genetic characterization of the Lr34/Yr18 slow rusting resistance gene region in wheat. Theor. Appl. Genet. 114:21–30.CrossRefGoogle Scholar
  19. Lander, E.S., Green, P., Abrahamsom, J., Barlow, A., Daly, M.J., Linsoln, S.E., Newbrug, L.A. 1987. MAPMAKER: An interactive computer package for construction of primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181.CrossRefGoogle Scholar
  20. Li, G.R., Yang, Z.J., Liu, M.J. 2000. Utilization of CIMMYT germplasm to Sichuan wheat breeding for quality. J. Sichuan Agricultural University 18:210–214.Google Scholar
  21. Maia, N. 1967. Obtention des bles tendres resistants au pietin-verse par croisements interspecifiques bles × Aegilops. C. R. Acad. Agric. Fr. 53:149–154 (in French).Google Scholar
  22. McIntosh, R.A., Wellings, C.R., Park, R.F. 1995. Wheat Rust — An Atlas of Resistance Genes. CSIRO Publications, Melbourne, 200 pp.CrossRefGoogle Scholar
  23. Michelmore, R.M., Paran, I., Kesseli, R.V. 1991. Identification of markers linked to disease-resistance genes by bulked segregant analysis: A rapid method to detect markers in specific genomic regions by using segregating populations. Proc. Natl. Acad. Sci. USA 88:9828–9832.CrossRefGoogle Scholar
  24. Moore, G., Devos, K.M., Wang, Z., Gale, M.D. 1995. Grasses, line up and form a circle. Curr. Biol. 5:737–739.CrossRefGoogle Scholar
  25. Plaschke, J.B., Börner, A., Wendehake, K., Ganal, M.W., Röder, M.S. 1996. The use of wheat aneuploids for the chromosomal assignment of microsatellite loci. Euphytica 89:33–40.CrossRefGoogle Scholar
  26. Ramakrishna, W., Dubcovsky, J., Park, Y.J., Busso, C., Emberton, J., SanMiguel, P., Bennetzen, J.L. 2002. Different types and rates of genome evolution de tected by comparative sequence analysis of orthologous segments from four cereal genomes. Genetics 162:1389–1400.PubMedPubMedCentralGoogle Scholar
  27. Rhoné, B., Raquin, A.L., Goldringer, I. 2007. Strong linkage disequilibrium near the selected Yr17 resistance gene in a wheat experimental population. Theor. Appl. Genet. 114:787–802.CrossRefGoogle Scholar
  28. Robert, O., Abelard, C., Dedryver, F. 1999. Identification of molecular markers for the detection of the yellow rust resistance gene Yr17 in wheat. Mol. Breeding 5:167–175.CrossRefGoogle Scholar
  29. Röder, M.S., Korzun, V., Wendehake, K., Plaschke, J., Tixier, M.H., Leroy, P., Ganal, M.W. 1998. A microsatellite map of wheat. Genetics 149:2007–2023.PubMedPubMedCentralGoogle Scholar
  30. Seah, S., Bariana, H., Jahier, J., Sivasithamparam, K., Lagudah, E.S. 2001. The introgressed segment carrying rust resistance genes Yr17, Lr37 and Sr38 in wheat can be assayed by a cloned disease resistance gene-like sequence. Theor. Appl. Genet. 102:600–605.CrossRefGoogle Scholar
  31. Xue, S., Zhang, Z., Lin, F., Kong, Z., Cao, Y., Li, C., Yi, H., Mei, M., Zhu, H., Wu, J., Xu, H., Zhao, D., Tian, D., Zhang, C., Ma, Z. 2008. A high-density intervarietal map of the wheat genome enriched with markers derived from expressed sequence tags. Theor. Appl. Genet. 117:181–189.CrossRefGoogle Scholar
  32. Yan, L., Loukoianov, A., Tranquilli, G., Helguera, M., Fahima, T., Dubcovsky, J. 2003. Positional cloning of the wheat vernalization gene VRN1. Proc. Natl. Acad. Sci. USA 100:6263–6268.CrossRefGoogle Scholar
  33. Yang, Z.J., Li, G.R., Feng, J., Jiang, H.R., Ren, Z.L. 2005. Molecular cytogenetic characterization and disease resistance observation of wheat-Dasypyrum breviaristatum partial amphiploid and its derivatives. Hereditas 142:80–85.CrossRefGoogle Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2011

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • J. Q. Jia
    • 1
  • G. R. Li
    • 1
  • C. Liu
    • 1
  • M. P. Lei
    • 1
  • Z. J. Yang
    • 1
    Email author
  1. 1.School of Life Science and TechnologyUniversity of Electronic Science and Technology of ChinaChengduP. R. China

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