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Cereal Research Communications

, Volume 39, Issue 1, pp 1–11 | Cite as

RT-PCR detection of the expression of COP1 and SINAT5 E3 ubiquitin ligase genes in different organs of Zea mays L.

  • A. GholizadehEmail author
  • B. Kohnehrouz
Open Access
Genetics

Abstract

Using bioinformatic data, we found that maize homologues of the Arabidopsis single subunit RING type ubiquitin ligase genes, COP1 (Constitutive Photomorphogenesis 1) and SINAT5 (Seven in Absentia in Arabidopsis thaliana 5) have been frequently predicted during large-scale cDNA sequencing project and released to databases since 2008. Despite this general information, no tissue specific expression profiles of these genes were published to our knowledge so far. In the present research, the expression and the relative levels of COP1 and SINAT5 transcripts were detected using reverse transcription and polymerase chain reaction (RT-PCR) with template materials collected separately from three different organs (root, leaf and seed) of maize plants grown under the normal growth conditions. The results not only confirmed the presence of COP1 transcript in all three test samples but also revealed its abundance in root tissues that may be consistent to its large number of targets for ubiquitination in darkness. Analysis of SINAT5 expression profile revealed a detectable band in leaf tissue sample that may be related to its specific roles in this organ.

Keywords

expression profile E3 ubiquitin ligase COP1 SINAT5 maize 

References

  1. Alexandrov, N.N., Brover, V.V., Freidin, S., Troukhan, M.E., Tatarinova, T.V., Zhang, H., Swaller, T.J., Lu, Y.P., Bouck, J., Flavell, R.B., Feldmann, K.A. 2008. Insights into corn genes derived from large-scale cDNA sequencing. Plant Mol. Biol. 69:179–194.CrossRefGoogle Scholar
  2. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seiolman, J.G., Smith, J.A., Struhi, R. 1991. Current Protocols in Molecular Biology. John Wiley and Sons, New York, USA.Google Scholar
  3. Bachmair, A., Novatchkova, M., Postuschak, T., Eisenhaber, F. 2001. Ubiquitylation in plants: a post genomic look at a post-translational modification. Trends Plant Sci. 6:463–470.CrossRefGoogle Scholar
  4. Bates, P.W., Vierstra, R.D. 1999. UPL1 and 2, Two 450 kDa ubiquitin protein ligases from Arabidopsis thaliana related to the HECT domain protein family. Plant J. 20:183–195.CrossRefGoogle Scholar
  5. Chiechanover, A. 1998. The ubiquitin-proteasome pathway: on protein death and cell life. EMBO J. 17:7151–7160.CrossRefGoogle Scholar
  6. Deng, X.W., Quail, P.H. 1992. Genetic and phenotypic characterization of COP1 mutants of Arabidopsis thaliana. Plant J. 2:83–95.CrossRefGoogle Scholar
  7. Downes, B.P., Stupar, R.M., Gingerich, D.J., Vierstra, R.D. 2003. The HECT ubiquitin protein ligase (UPL) family in Arabidopsis: UPL3 has a specific role in trichome development. Plant J. 3:729–742.CrossRefGoogle Scholar
  8. Freemont, P.S., Hanson, I.M., Trowsdale, J.A. 1991. A novel cysteine-rich sequence motif. Cell 64:483–484.CrossRefGoogle Scholar
  9. Hellmann, H., Estelle, M. 2002. Plant development: regulation by protein degradation. Science 297:793–797.CrossRefGoogle Scholar
  10. Hershko, A., Chiechanover, A. 1998. The ubiquitin system. Annu. Rev. Biochem. 67:425–479.CrossRefGoogle Scholar
  11. Huibregtse, J.M., Scheffiner, M., Beaudenon, S., Howley, P.M. 1995. A family of proteins structurally and functionally related to the E6-AP ubiquitin protein ligase. Proc. Natl. Acad. Sci. USA 92:2563–2567.CrossRefGoogle Scholar
  12. Koegl, M., Hoppe, T., Schlenker, S., Ulrich, H.D., Mayer, T.U., Jentsch, S.A. 1999. A novel ubiquitination factor, E4, involved in multiubiquitin chain assembly. Cell 96:635–644.CrossRefGoogle Scholar
  13. Kosarev, P., Mayer, K.F.X., Hardtke, C.S. 2002. Evaluation and classification of RING finger domains encoded by the Arabidopsis genome. Genome Biol. 3:0016.1–0016.12.CrossRefGoogle Scholar
  14. Lorick, K.L., Jenson, J.P., Fang, S., Ong, A.M., Hatakeyama, S., Weissman, A.M. 1999. RING fingers mediate ubiquitin-conjugating enzyme (E2)-dependent ubiquitination. Proc. Natl. Acad. Sci. USA 96:11364–11369.CrossRefGoogle Scholar
  15. Ma, L., Gao, Y., Qu, L., Chen, Z., Li, J., Zhao, H., Wang, X. 2002. Genomic evidence for COP1 as repressor of light regulated gene expression and development in Arabidopsis. Plant Cell 14:2383–2398.CrossRefGoogle Scholar
  16. Mazzucotelli, E., Belloni, S., Marone, D., De Leonardis, A.M., Guerra, D., Di Fonzo, N., Cattivelli, L., Mastrangelo, A.M. 2006. The E3 ubiquitin ligase gene family in plants: regulation by degradation. Curr. Genomics 7:509–522.CrossRefGoogle Scholar
  17. Moon, J., Parry, G., Estelle, M. 2004. The ubiquitin-proteasome pathway and plant development. Plant Cell 16:3181–3195.CrossRefGoogle Scholar
  18. Pickart, C.M. 2001. Mechanisms underlying ubiquitination. Annu. Rev. Biochem. 70:503–533.CrossRefGoogle Scholar
  19. Schwechheimer, C., Schwager, K. 2004. Regulated proteolysis and plant development. Plant Cell Rep. 23:353–364.CrossRefGoogle Scholar
  20. Seo, H.S., Watanabe, E., Tokutomi, S., Nagatani, A., Chua, N.H. 2004. Photoreceptor ubiquitination by COP1 E3 ligase desensitizes phytochrome A signaling. Genes Dev. 18:617–622.CrossRefGoogle Scholar
  21. Smalle, J., Vierstra, R.D. 2004. The ubiquitin 26s proteasome proteolytic pathway. Annu. Rev. Plant Physiol. Plant Mol. Biol. 55:555–590.CrossRefGoogle Scholar
  22. Stone, S.L., Hauksdottir, H., Tory, A., Herschleb, J., Kraft, E., Callis, J. 2005. Functional analysis of the RING-type ubiquitin ligase family of Arabidopsis. Plant Physiol. 137:13–30.CrossRefGoogle Scholar
  23. Su, L., Lineberry, N., Huh, Y., Soares, L., Fathman, C.G. 2006. Anovel E3 ubiquitin ligase substrate screen identities Rho guanine dissociation inhibitor as a substrate of gene related to anergy in lymphocytes. J. Immunol. 177:7559–7566.CrossRefGoogle Scholar
  24. Xie, Q., Gue, H.S., Dallman, G., Fang, S., Weissman, A.M., Chua, N.H. 2002. SINAT5 promotes ubiquitin related degradation of NAC1 to attenuate auxin signals. Nature 419:167–170.CrossRefGoogle Scholar
  25. Yi, C., Deng, X.W. 2005. COP1: from plant photomorphogenesis to mammalian tumorigenesis. Trends Cell Biol. 15:618–625.CrossRefGoogle Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2011

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  1. 1.Institute for Fundamental Sciences (RIFS)University of Tabriz ResearchTabrizIran
  2. 2.Department of Plant Breeding and BiotechnologyUniversity of TabrizTabrizIran

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