Characterization of wheat (Triticum aestivum L.) cultivars grown in Lesotho by storage proteins
Thirty bread wheat cultivars grown in Lesotho were analysed with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). High and low molecular weight glutenin subunits (HMW-GS and LMW-GS) and gliadins were used to determine genetic variability and relationships between the cultivars. The HMW-GS could not distinguish the cultivars, while both LMW-GS and gliadins could. The Glu-1 score showed that all the quality classes from excellent to poor were represented in the material, but 11 cultivars had the highest score of 10, indicating excellent quality. Cluster analysis performed using gliadins in combination with LMW-GS generated dendrograms which segregated cultivars according to genetic distance. However, the genetic distance between the cultivars were so close that it could be concluded that they are from the same gene-pool and have been used several times in regional breeding programmes.
Keywordsbread wheat gliadin HMW-GS LMW-GS SDS-PAGE
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