Allelic diversity of HMW and LMW glutenin subunits in Chinese Rare Dwarf-Male-Sterile wheat (Triticum aestivum L.)
The dwarf-male-sterile wheat is unique to China and has been improved by introducing good germplasm. In order to clear the subunits background of Dwarf-Male-Sterile wheat, sodium dodecyl sulphate polyacrylamide-gel electrophoresis (SDS-PAGE) was used to detect the high and low molecular weight glutenin subunits (HMW-GS and LMW-GS) compositions in BC1F1, F2 and F3 generations from Dwarf-Male-Sterile wheat. Twenty-five alleles and 49 HMW-GS compositions at the Glu-1 loci were detected in different generations. Null and subunit 1 were mainly existed at Glu-A1, and 7 + 8 and 7 + 9 were primarily detected at Glu-B1 in different generations. Subunit combination 5 + 10 mainly appeared in BC1F1, while 2 + 12 major presented in F2 and F3 generations. HMW-GS compositions null, 7 + 8, 5 + 10 and null, 7 + 9, 5 + 10 showed higher frequencies than other banding patterns, followed by null, 14 + 15, 5 + 10 and null, 7 + 9, 2 + 12 combinations. In addition, some rare subunit combinations such as 14 + 15, 13 + 16, 17 + 18, 4 + 12, 2 + 10 and 5 + 12 were found in different generations. Eighteen alleles and 51 LMW-GS compositions at Glu-3 loci were found in different generations. Glu-A3 a and Glu-B3 d showed higher frequencies than others among three generations. There were mainly a, b, c alleles at Glu-D3. Thirty, 31 and 14 different combinations were detected in BC1F1, F2 and F3 populations, respectively. There were some good combinations such as A3 d/ B3 h, A3 d/ B3 d/ D3 a, A3 b/ B3 b/ D3 a, A3 a/ B3 d/ D3 a for different quality characteristics. So some desirable subunit combinations could be selected from different generations and new cultivars with good quality under distinct subunits background should be bred from Dwarf-Male-Sterile wheat in future.
KeywordsChinese Dwarf-Male-Sterile wheat HMW-GS LMW-GS different generations
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