Development of a molecular marker for simultaneous selection of Rph7 gene and effective Mla alleles in barley

Abstract

Barley (Hordeum vulgare L.) is one of the most economically important crops around the world. Diseases caused by fungi and viruses significantly reduce yield. The most important fungal diseases are leaf rust caused by Puccinia hordei, and powdery mildew caused by Blumeria graminis f. sp. hordei. The most economical and ecologically friendly way to avoid losses caused by these diseases is growing resistant varieties. Concerning practical breeding for powdery mildew and leaf rust resistance, simple, cheap and robust selection methods are required. Marker assisted selection (MAS) is of great potential to fulfil this demand. In this paper, we present development of a robust duplex marker for simultaneous selection of Rph7, and presence of one of the following Mla alleles: Mla16, Mla19, Mla20, Mla21, Mla27 or Mla28.

References

  1. Ayliffe, M.A., Lagudah, E.S. 2004. Molecular genetics of disease resistance in cereals. Ann. Bot. 94:765–773.

    CAS  Article  Google Scholar 

  2. Brunner, S., Keller, B., Feuilet, C. 2000. Molecular mapping of the Rph7.g leaf rust resistance gene in barley (Hordeum vulgare L.). Theor. Appl. Genet. 101:783–788.

    CAS  Article  Google Scholar 

  3. Buschges, R., Hollricher, K., Panstruga, R., Simons, G., Wolter, M., Frijters, A., van Daelen, R., van der Lee, T., Diergaarde, P., Groenendijk, J., Topsch, S., Vos, P., Salamini, F., Schulze-Lefert, P. 1997. The barley Mlo gene: a novel control element of plant pathogen resistance. Cell 88:695–705.

    CAS  Article  Google Scholar 

  4. Chełkowski, J., Tyrka, M., Sobkiewicz, M. 2003. Resistance genes in barley (Hordeum vulgare L.) and their identification with molecular markers. J. Appl. Genet. 44:291–309.

    PubMed  Google Scholar 

  5. Czembor, J.H. 1996. Presence and expression of resistance genes to powdery mildew of barley in selections from Tunisian barley landraces. Ph.D. thesis, Department of Plant Pathology, Montana State University, Bozeman, USA.

  6. Czembor, J.H., Talbert, L.E. 1997. Evaluation of STS-PCR and RFLPs as molecular markers for the Mla locus conferring powdery mildew resistance in barley. Plant Breed. Seed Sci. 41:1–14.

    Google Scholar 

  7. Czembor, H.J., Czembor, J.H. 2001. Resistance to powdery mildew in barley cultivars and breeding lines included in 1998–2000 Polish registration trials. Plant Breed. Seed Sci. 45:19–28.

    Google Scholar 

  8. Dreiseitl, A. 2003. Adaptation of Blumeria graminis f. sp. hordei to barley resistance genes in the Czech Republic in 1971–2000. Plant Soil Environ. 49:241–248.

    Article  Google Scholar 

  9. Franckowiak, J.D., Jin, Y., Steffenson, B.J. 1997. Recommended allele symbols for leaf rust resistance genes in barley. Barley Genet. Newsl. 27:36–44.

    Google Scholar 

  10. Golan, T., Anikster, Y., Moseman, J.G., Wahl, I. 1978. A new virulent strain of Puccinia hordei. Euphytica 27:185–189.

    Article  Google Scholar 

  11. Graner, A., Streng, S., Drescher, A., Jin, Y., Borovkova, I., Steffenson, B.J. 2000. Molecular mapping of the leaf rust resistance gene Rph7 in barley. Plant Breeding 119:389–392.

    CAS  Article  Google Scholar 

  12. Griffey, C.A., Das, M.K., Baldwin, R.E., Waldenmaier, C.M. 1994. Yield losses in winter barley resulting from new race of Puccinia hordei in North America. Plant Dis. 78:256–260.

    Article  Google Scholar 

  13. Gupta, P.K., Varshney, R.K., Sharma, P.C., Ramesh, B. 1999. Molecular markers and their applications in wheat breeding. Plant Breeding 118:369–390.

    CAS  Article  Google Scholar 

  14. Halterman, D.A., Wei, F., Wise, R.P. 2003. Powdery mildew-induced Mla mRNAs are alternatively spliced and contain multiple upstream open reading frames. Plant. Physiol. 131:558–567.

    CAS  Article  Google Scholar 

  15. Hovmöller, M.S., Caffier, V., Jalli, M., Andersen, O., Besenhofer, G., Czembor, J.H., Dreiseitl, A., Flath, K., Fleck, A., Heinrics, F., Jönsson, R., Limpert, E., Mercer, P., Plesnik, S., Rashal, I., Skinnes, H., Slater, S., Vronska, O. 2000. The European barley powdery mildew virulence survey and disease nursery 1993–1999. Agronomie 20:729–744.

    Article  Google Scholar 

  16. Jahoor, A., Fischbeck, G. 1993. Identification of new genes for mildew resistance of barley at the Mla locus in lines derived from Hordeum spontaneum. Plant Breeding 110:116–122

    Article  Google Scholar 

  17. Keb-Llanes, M., González, G., Chi-Manzareno, B., Infante, D. 2002. A rapid and simple method for small-scale DNA extraction in Agavaceae and other tropical plants. Plant Molec. Biol. Reporter 3:299a–299e.

    Article  Google Scholar 

  18. Kintzios, S., Fischbeck, G. 1996. Identification of new sources for resistance to powdery mildew in H. spontaneum -derived winter barley lines. Genet. Resour. and Crop Evol. 43:25–31

    Article  Google Scholar 

  19. Mammadov, J.A., Brooks, W.S., Griffey, C.A., Saghai Maroof, M.A. 2007. Validating molecular markers for barley leaf rust resistance genes Rph5 and Rph7. Plant Breeding 126:458–463.

    CAS  Article  Google Scholar 

  20. Mejlhede, N., Kyjovska, Z., Backes, G., Burhenne, K., Rasmussen, S. K., Jahoor, A. 2006. Ecotilling for the identification of allelic variation in the powdery mildew resistance genes mlo and Mla of barley. Plant Breeding 125:461–467.

    CAS  Article  Google Scholar 

  21. Ordon, F., Wenzel, W., Friedt, W. 1998. II Recombination: molecular markers for resistance genes in major grain crops. Prog. Bot. 59:49–79.

    CAS  Article  Google Scholar 

  22. Panstruga, R., Molina-Cano, J., Reinstädler, A., Müller, J. 2005. Molecular characterization of mlo mutants in North American two- and six-rowed maling barley cultivars. Mol. Plant Pathol. 6:315–320.

    CAS  Article  Google Scholar 

  23. Park, R.F., Karakousis, A. 2002. Characterization and mapping of the gene Rph19 conferring resistance to Puccinia hordei in the cultivar “Reka I” and several Australian barleys. Plant Breeding 121:232–236.

    CAS  Article  Google Scholar 

  24. Parlevliet, J.E. 1976. The genetics of seedling resistance to leaf rust, Puccinia hordei Otth in some spring barley cultivars. Euphytica 25:249–254.

    Article  Google Scholar 

  25. Perovic, D., Welz, G., Förster, J., Kopahnke, D., Lein, V., Löschenberger, F., Buerstmayr, H., Ordon, F. 2009. Breeding strategies for wheat improvement: creating semi-dwarf phenotypes with superior fusarium head blight resistance. In: Feldmann, F., Alford, D.V., Furk, C. (eds), Crop Plant Resistance to Biotic and Abiotic Factors (2009), ISBN 978-3-941261-05-1; Deutsche Phytomedizinische Gesellschaft, Braunschweig, Germany. pp. 456–461.

    Google Scholar 

  26. Roane, C.W., Starling, T.M. 1970. Inheritance of reaction to puccinia hordei in barley. III Genes in the cultivars “Cebada Capa” and “Franger”. Phytopathol. 60:788–790.

    Article  Google Scholar 

  27. Steffenson, B.J., Jin, Y., Griffey, C.A. 1993. Pathotypes of Puccinia hordei with virulence for the barley leaf rust resistance gene Rph7 in the United States. Plant Dis. 77:867–869.

    Article  Google Scholar 

  28. Thomas, W.T.B. 2002. Molecular marker-assisted versus conventional selection in barley breeding. In: Slafer, G.A., Molina-Cano, J.L., Savin, R., Araus, J.L., Romagosa, I. (eds), Barley Science: Recent Advances from Molecular Biology to Agronomy of Yield and Quality. Food Product Press, New York, USA. An Imprint of Haworth Press, Inc., New York, USA. pp. 177–205.

    Google Scholar 

  29. Wei, F., Gobelman-Werner, K., Morroll, S.M., Kurth, J., Mao, L., Wing, R., Leister, D., Schulze-Lefert, P., Wise, R.P. 1999. The Mla (powdery mildew) resistance cluster is associated with three NBS-LRR gene families and suppressed recombination with in a 240-kb DNA interval on chromosome 5S (1HS) of barley. Genetics 153:1929–1948.

    CAS  PubMed  PubMed Central  Google Scholar 

  30. Wei, F., Wing, R.A., Wise, R.P. 2002. Genome dynamics and evolution of the Mla (Powdery Mildew) resistance locus in barley. Plant Cell 14:1903–1917.

    CAS  Article  Google Scholar 

  31. Williams, J.K. 2003. The molecular genetics of disease resistance in barley. Aust. J. Agric. Res. 54:1065–1079.

    CAS  Article  Google Scholar 

  32. Zeven, A.C. 1996. Results of activities to maintain landraces and other material in some European countries in situ before 1945 and what we may learn from them. Genet. Resour. Crop Evol. 43:337–341.

    Article  Google Scholar 

  33. Zhou, F., Kurth, J., Wei, F., Elliott, C., Vale, G., Yahiaoui, N., Keller, B., Somerville, S., Schulze-Lefert, P. 2001. Cell-autonomous expression of barley Mla-1 confers race-specific resistance to the powdery mildew fungus via a Rar1-independent signalling pathway. Plant Cell 13:337–350.

    CAS  Article  Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to T. Sedláček.

Additional information

Communicated by H. Bürstmayr

Rights and permissions

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Reprints and Permissions

About this article

Cite this article

Sedláček, T., Stemberková, L. Development of a molecular marker for simultaneous selection of Rph7 gene and effective Mla alleles in barley. CEREAL RESEARCH COMMUNICATIONS 38, 175–183 (2010). https://doi.org/10.1556/CRC.38.2010.2.3

Download citation

Keywords

  • barley
  • resistance
  • powdery mildew
  • leaf rust
  • molecular marker
  • CAPS