Barley-infecting isolates of Wheat dwarf virus (WDV) were collected in the field in the vicinity of the cities Dunakiliti, Heves and Siófok, in Hungary. Viral genomic DNA was amplified by the rolling circle amplification technique, digested with HindIII, cloned into pBSK+ plasmid and sequenced. The clones were of the same size and showed above 99% identity to each other. Based on DNA sequences WDV-D01, WDV-H1 and WDV-H07 isolates showed high identity (94–99%) to isolates of WDV barley strain and Barley dwarf virus and lower identity to Oat dwarf virus (71% identity) and WDV wheat strains (85% identity).
Achon, M.A., Serrano, L., Ratti, L., Rubies-Autonell, C. 2006. First detection of Wheat dwarf virus in barley in Spain associated with an outbreak of barley yellow dwarf. Plant Disease 90: 970.
Bendahmane, M., Schalk, H-J., Gronenborn, B. 1995. Identification and characterization of wheat dwarf virus from France using a rapid method for geminivirus DNA preparation. Phytopathol. 85: 1449–1455.
Bisztray, Gy., Gáborjányi R., Vacke, J. 1989. Isolation and characterisation of wheat dwarf virus found for the first time in Hungary. J. Plant Dis. Protect. 96: 449–454.
Gutierrez, C. 1999. Geminivirus DNA replication. Cell Mol. Life Sci. 56: 313–329.
Haible, D., Kober, S., Jeske, H. 2006. Rolling circle amplification revolutionizes diagnosis and genomics of geminiviruses. J. Virological Methods 135: 9–16.
Heyraud, F., Matzeit, V., Kammann, M., Schaefer, S., Schell, J., Gronenborn, B. 1993. Identification of the initiation sequence for viral-strand DNA synthesis of wheat dwarf virus. EMBO J. 12: 4445–4452.
Huth, W. 2000. Viruses of Gramineae in Germany — a short overview. J. Plant Dis. Protect. 107: 406–414.
Jezewska, J. 2001. First report of Wheat dwarf virus occurring in Poland. Phytopathologica Polonica 21: 93–100.
Jilaveanu, A., Vacke, J. 1995. Isolation and identification of wheat dwarf virus (WDV) in Romania. Probleme de Protectia Plantelor 23: 51–62.
Kammann, M., Schalk H.J., Matzeit, V., Schaefer, S., Schell, J., Gronenborn, B. 1991. DNA replication of wheat dwarf virus, a geminivirus, requires two cis-acting signals. Virology 184: 786–790.
Kapooria, R.G., Ndunguru, J. 2004. Occurrence of viruses in irrigated wheat in Zambia. EPPO/OEPP Bulletin 34: 413–419.
Köklü, G., Ramsell, J.N.E., Kvarnheden, A. 2007. The complete genome sequence for a Turkish isolate of Wheat dwarf virus (WDV) from barley confirms the presence of two distinct WDV strains. Virus Genes 34: 359–366.
Kvarnheden, A., Lindblad, M., Lindsten., K., Valkonen, J.P.T. 2002. Genetic diversity of Wheat dwarf virus. Arch. Virol. 147: 205–216.
Lemmetty, A., Huusela-Veistola, E. 2005. First report of Wheat dwarf virus in winter wheat in Finland. Plant Disease 89: 912.
Lindsten, K., Lindsten, B. 1993. Occurrence and transmission of Wheat dwarf virus (WDV) in France. In: Proceedings of Third International Conference on Pest in Agriculture. Montpellier, France, 7–9 December 1993, pp. 41–48.
Lindsten, K., Vacke, J. 1991. A possible barley adapted strain of Wheat dwarf virus (WDV). Acta Phytopath. Entomol. Hung. 26: 175–180.
Lindsten, K., Vacke, J., Gerhardson, B. 1970. A preliminary report on three cereal virus diseases new to Sweden spread by Macrosteles and Psammotettix leafhoppers. Nat. Swedish Inst. Plant Protec. Cent. 14: 285–297.
Mesterházy, Á., Gáborjányi, R., Papp, M., Fónad, P. 2002. Multiple virus infection of wheat in South Hungary. Cereal Res. Commun. 30: 329–334.
Najar, A., Makkouk, K.M., Boudhir, H., Kumari, S.G., Zarouk, R., Bessai, R., Othman, F.B. 2000. Viral diseases of cultivated legume and cereal crops in Tunisia. Phytopathologia Mediterranea 39: 423–432.
Sambrook, J., Fritsch, E.F., Maniatis, T. 1989. Molecular Cloning. A Laboratory Manual. 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
Schubert, J., Habekuss, A., Kazmaier, K., Jeske, H. 2007. Surveying cereal-infecting geminiviruses in Germany — Diagnostics and direct sequencing using rolling circle amplification. Virus Research 127: 61–70.
Shepherd, D., Martin, P.D., Lefeuvre, P., Monjane, A., Owor, B., Rybicki, E.P., Varsani, A. 2008. A protocol for the rapid isolation of full geminivirus genomes from dried plant tissue. J. Virological Methods 149: 97–102.
Stephanov, J., Dimov, A. 1981. Bolestta vdjudjavanje po spenittsata Bolgaria (Dwarf disease on wheat in Bulgaria). Rasteniev Nauki 18: 124–128.
Szunics, L., Pocsai, E., Vida, G., Veisz, O., Láng, L., Bedő, Z. 2003. Kalászos gabonák vírusok okozta betegségei 2002-ben (Virus diseases on cereals in 2002). Növénytermelés 52: 33–39.
Tóbiás, I., Kiss, B., Palkovics, L. 2006. The nucleotide sequence of two Hungarian isolates of Wheat dwarf virus. Acta Phytopath. et Entomol. Hung. 41: 47–52.
Tóbiás, I., Kiss, B., Bakardjieva, N., Palkovics, L. 2009. The nucleotide sequence of barley strain of Wheat dwarf virus isolated in Bulgaria. Cereal Res. Commun. 37: 237–242.
Vacke, J. 1961. Wheat dwarf virus. Biol. Plant. 3: 228–233.
Xie, J., Wang, X., Liu, Y., Peng, Y., Zhou, G. 2007. First report of the occurrence of Wheat dwarf virus in wheat in China. Plant Dis. 91: 111.
About this article
Cite this article
Tóbiás, I., Kiss, B., Salánki, K. et al. The nucleotide sequence of barley strain of Wheat dwarf virus isolated in Hungary. CEREAL RESEARCH COMMUNICATIONS 38, 67–74 (2010). https://doi.org/10.1556/CRC.38.2010.1.7
- Wheat dwarf virus
- Barley dwarf virus
- Oat dwarf virus
- barley-infecting isolates
- sequence analysis