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Cereal Research Communications

, Volume 38, Issue 1, pp 43–55 | Cite as

Changes of antioxidative enzymes in salinity tolerance among different wheat cultivars

  • A. A. Abdel LatefEmail author
Physiology

Abstract

The response of three wheat (Triticum aestivum L.) cultivars Banysoif 1 (C1), Sakha 68 (C2) and Seds 1 (C3) to salinity stress (−1.11 MPa NaCl) at germination and early seedling growth was investigated. According to the germination, dry weight production and tissue water content, C1 seemed to be more or less unaffected by salinity, whereas C3 was severely reduced and C2 was almost intermediated. Consequently, carbohydrate, protein and free amino acids contents were increased in C1 and C2, while the opposite occurred in C3 (except soluble proteins and free amino acids). On the other hand, while proline content decreased in C2 and C3, it markedly increased in C1 as a result of salinity stress. Na+/K+ ratio was higher in C3 than in C1. C2 was intermediate. Significant increase in SOD activity was observed in seedlings of C1 and C2. On the other hand, SOD activity was markedly decreased in C3 cultivar. Seedling extracts exhibited three SOD activity bands (SOD1, SOD2 and SOD3) in C1 and C2. While in C3 seedling, only two SOD activity bands (SOD1 and SOD3) were identified, whereas the SOD2 isozyme was not expressed under control or NaCl conditions in this cultivar. Salinity stress significantly increased POD activity in C1 and C3, but it markedly decreased the activity of POD in C2. Two isozymes of POD (POD1 and POD2) were observed in all groups of C1. The intensity and density of POD1 and POD2 markedly increased under salinity stress versus control group. In C2, salinity stress resulted in disappearance of POD1 as compared with control group. In C3, salinity stress induced the appearance of POD1 which disappear under control group. CAT activity in C1 and C2 was markedly increased under NaCl salinity. On the other hand, CAT activity was markedly decreased in C3. NaCl salinity did not affect APX activity in three wheat cultivars. In addition, lipid peroxidation level of salt-sensitive C3 markedly increased, indicating more damage to membrane lipids due to −1.11 MPa NaCl. Lipid peroxidation did not change in the salt-tolerant C1 at the same concentration of NaCl. C2 was intermediate. These results suggest that at seedling stage, C1 is appeared to be more tolerant than C2 and C3 under salinity stress.

Keywords

dry weight production germination ratio physiological changes isoenzymes patterns salinity tolerance wheat cultivars 

Abbreviations

APX

Ascorbate peroxidase

C1

Banysoif 1

CAT

Catalase

cvs

Cultivars

DW

Dry weight

FW

Fresh weight

MDA

Malondialdehyde

POD

Peroxidase

PAGE

Polyacrylamide gel electrophoresis

ROS

Reactive oxygen species

C2

Sakha 68

C3

Seds 1

SOD

Superoxide dismutase

WC

Water content

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References

  1. Afzal, I., Basrai, S.M.A., Hamed, A., Farooqi, M. 2006. Physiological enhancement for alleviation of salt stress in wheat. Pak. J. Bot. 38: 1649–1659.Google Scholar
  2. Ardıc, M., Sekmen, H., Turkan, I., Tokur, S., Ozdemir, F. 2009. The effects of boron toxicity on root antioxidant systems of two chickpea (Cicer arietinum L.) cultivars. Plant Soil 314: 99–108.CrossRefGoogle Scholar
  3. Ashraf, M. 1994. Organic substances responsible for salt tolerance in Eruca sativa. Biol. Plant. 36: 255–259.CrossRefGoogle Scholar
  4. Ashraf, M. 1999. Breeding for salinity tolerance proteins in plants. Crit. Rev. Plant. Sci. 13: 17–42.CrossRefGoogle Scholar
  5. Azooz, M.M., Ismail, A.M., Abou Elhamd, M.F. 2009. Growth, lipid peroxidation and antioxidant enzyme activities as a selection criterion for the salt tolerance of maize cultivars grown under salinity stress. Int. J. Agri. Biol. 11: 21–26.Google Scholar
  6. Badour, S.S.A. 1959. Analytisch-chemische Untersuchung des Kaliummangels bei Chlorella im Vergleich mit anderen Mangelzuständen; Ph.D. Dissertation Göttingen. (In German)Google Scholar
  7. Bates, L.S., Wladren, R.P., Tear, L.D. 1973. Rapid determination of free proline for water-stress studies. Plant Soil 39: 205–207.CrossRefGoogle Scholar
  8. Bergmeyer, H. U. 1970. Methoden der enzymatischen Analyse, vol. 1. Verlag Chemie, Weinheim/Bergstr., Germany, pp. 636–647.Google Scholar
  9. Beuchamp, C., Fridovich, I. 1971. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal. Biochem. 44: 276–287.CrossRefGoogle Scholar
  10. Beuchamp, C., Fridovich, I. 1973. Isozymes of superoxide dismutase from wheat germ. Biochem. Biophys. Acta 317: 50–64.Google Scholar
  11. Boo, Y.C., Jung, J. 1999. Water deficit-induced oxidative stress and antioxidative defenses in rice plants. J. Plant Physiol. 155: 255–261.CrossRefGoogle Scholar
  12. Borsani, O., Valpuesta, V., Botella, M.A. 2003. Developing salt tolerant plants in a new century: A molecular biology approach. Plant Cell Tiss. Org. Cult. 73: 101–115.CrossRefGoogle Scholar
  13. Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein binding. Anal. Biochem. 72: 248–254.CrossRefGoogle Scholar
  14. Chatzissavvidis, C., Veneti, G., Papadakis, I., Therios, I. 2008. Effect of NaCl and CaCl2 on the antioxidant mechanism of leaves and stems of the rootstock CAB-6P (Prunus cerasus L.) under in vitro condition. Plant Cell Tiss. Organ. Cult. 95: 37–45.CrossRefGoogle Scholar
  15. Foolad, M.R. 1996. Genetic analysis of salt tolerance during vegetative growth in tomato, Lycopersicon esculentum Mill. Plant Breeding 115: 245–250.CrossRefGoogle Scholar
  16. Hare, P.D., Cress, W.A., van Staden, J. 1998. Dissecting the roles of osmolyte accumulation during stress. Plant Cell Environ. 21: 535–554.CrossRefGoogle Scholar
  17. Herzog, V., Fahimi, H. 1973. Determination of the activity of peroxidase. Anal. Biochem. 55: 554–562.CrossRefGoogle Scholar
  18. Jaleel, C.A., Gopi, R., Gomthinayagam, M., Panneerselvam, R. 2008. Effects of calcium chloride on metabolism of salt-stressed Dioscorea rotundata. Acta Biol. Cracovein. Series. Bota. 50: 63–67.Google Scholar
  19. Lee, Y.P., Takanashi, T. 1966. An improved coloremeteric determination of amino acids with the use of ninhydrin. Anal. Biochem. 14: 71–77.CrossRefGoogle Scholar
  20. Madhava Rao, K.V., Sresty, T.V.S. 2000. Antioxidative parameters in the seedlings of pigeonpea (Cajanus cajan L. Millspaugh) in response to Zn and Ni stresses. Plant Sci. 157: 113–128.CrossRefGoogle Scholar
  21. Nakano, Y., Asada, K. 1981. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol. 22: 867–880.Google Scholar
  22. Rahman, M., Soomoro, U.A., Hag, M.Z., Gul, S. 2008. Effects of NaCl salinity on wheat (Triticum aestivum L.) cultivars. World J. Agri. Sci. 4: 398–403.Google Scholar
  23. Seevers, F.M., Daly, J.M., Catedral, F.F. 1971. The role of peroxidase isozymes in resistance to wheat stem rust. Plant Physiol. 48: 353–360.CrossRefGoogle Scholar
  24. Sreenivasulu, N., Grimma, B., Wobusa, U., Weschke, W. 2000. Differential response of antioxidant compounds to salinity stress in salt-tolerant and salt-sensitive seedlings of foxtail millet (Setaria italica). Physiol. Plant. 109: 435–442.CrossRefGoogle Scholar
  25. Sreenivasulu, N., Ramanjulu, S., Ramachandra, K., Prakash, H.S., Shetty, H.S., Savithri, H.S., Sudhakar, C. 1999. Peroxidase activity and peroxidase isoforms as modified by salt stress in two cultivars of foxtail millet. Plant Sci. 141: 1–9.CrossRefGoogle Scholar
  26. Wimmer, M.A., Muhling, K.H., Lauchli, A., Brown, P.H., Goldbach, H.E. 2003. The interaction between salinity and boron toxicity affects the subcellular distribution of ions and proteins in wheat leaves. Plant Cell Environ. 26: 1267–1274.CrossRefGoogle Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2010

Authors and Affiliations

  1. 1.Botany Department, Faculty of ScienceSouth Valley UniversityQenaEgypt

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